Báo cáo Y học: Characterization of the self-splicing products of two complex Naegleria LSU rDNA group I introns containing homing endonuclease genes pdf

... 2002 some intron systems that include the ORF -containing group I introns (Table 2). In vivo analyses of I- PpoI, I- DirI and I- NgrI expression in their original hosts and/or in yeast indicate an essential ... motif is directly involved in the two zinc ion coordination sites [5]. Whereas I- NaeI contains a His-Cys box typical of the two zinc binding motifs, the I-...

Ngày tải lên: 18/03/2014, 01:20

... abnormalities. 3. Complex (multifactorial) origin caused by gene- environmental interaction when the so-called polygenic liability (predisposition) is triggered by environmental ‘risk’ factors. ... proportion of genetic origin is estimated about 25 % of total congenital abnormalities. Mainly two conditions may contribute to a higher total prevalence of congenital abnormalit...

Ngày tải lên: 02/11/2012, 11:12

... visible. Similarly, the pronuclei in ovine zygotes are very difficult to visualise, due to sharing a very similar refractive index with the cytoplasm; this necessitates the use of top-quality optics, ... become the preferred therapeutic route. The possibility of human germline genetic modification raises several important and vexing bioethical issues, including questions of...

Ngày tải lên: 03/11/2012, 10:01

... all these enzymes catalyse an hydrolytic reaction with production of urea. The question arises therefore as to whether a similar or identical mechanism is involved in catalysis by these enzymes, ... bearing the speB gene of E. coli agmatinase, was kindly supplied by S. Boyle (Vir- ginina Polytechnic Institute and State University). The pQE60 E. coli expression vector and the Ni-ni...

Ngày tải lên: 21/02/2014, 01:21

... concen- trations of this compound, the incorporation of 14 C-label from the cyclic diphosphate into the lipid-soluble fraction was significantly diminished. In a similar experiment, we studied the formation of ... range coupling involving vicinal isoprenoid moieties. Due to the line broadening, the precision of signal integration is substan- tially reduced. However, within...

Ngày tải lên: 22/02/2014, 07:20

... the skewed continuous size distributions especially for the larger particles (Fig. 3). Interaction of ApoE(263–286) with lipid emulsions We initially examined the binding of a synthetic peptide comprising ... open conformation; lipid binding; analytical ultracentrifugation. Traditional methods for monitoring protein–lipid interac- tions have employed a variety of lipid systems, in...

Ngày tải lên: 08/03/2014, 09:20

... abnormality in high-risk groups may reflect an underlying state of CNS dis-inhibition involved in the pathophysiology of the condition [93]. Genetic linkage studies involving families with a history ... utilizing the auditory oddball paradigm. In one such study examining frequency and intensity deviation, changes in inter-stimulus interval (ISI) and intensity in auditory oddb...

Ngày tải lên: 02/11/2012, 11:08

... employed in the food industry as viscosifying, stabilizing, emulsifying and gelling agents [1]. The texturizing properties of EPSs in fermented dairy products [2] in combination with the GRAS ... bacterium. Due to the novel composition of the EPS produced by S. thermophilus 8S, the genetics and biochemistry of the EPS biosynthesis as well as the physical properties of...

Ngày tải lên: 08/03/2014, 09:20

... In plants, they may mainly play a role in lignification [4] whereas in fungi they probably play the opposite role, i. e. delignifica- tion [5,6], among many others [1]. C30 is a white-rot basidiomycete ... 5.7 instead of pH 5.0, the optimum pH for LAC1. Azide inhibition Sodium azide inhibition of either syringaldazine or ABTS oxidation was measured for LAC1 and LAC2. In both cases, thi...

Ngày tải lên: 08/03/2014, 09:20

... Internal Medicine, 5 Department of Gynecology, and 6 Department of Neurosurgery, Akita University School of Medicine, Akita City, Japan; 7 President’s Frontier Laboratory. Mitsubishi Kasei Institute of Life Sciences, Tokyo, ... studies using an anti-(rat 105-kDa protein) IgG. The localization of the 105-kDa protein in the rat brain is presented in Fig. 9. The protein is loca...

Ngày tải lên: 17/03/2014, 10:20