Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx

... Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants Lars Norderhaug 1 , Finn-Eirik Johansen 2 and Inger Sandlie 3 1 Antibody ... for production of total SC, by a dot blot approach. Triplets of Fig. 1. A schematic diagram of the fusion of cells producing individual protein units...

Ngày tải lên: 18/03/2014, 01:20

... 4.9, after a routine hema- tological investigation and after the assessment of radiographic exams, such as X-Ray Dental Panoramic Tomogram and Denta-Sca n (Fig. 6) of the inferior maxillary bone. ... “Fatebenefratelli”, Rome, Italy 4. Department of Maxillofacial Surgery, Calabrodental, Crotone, Italy 5. Department of Dental Sciences and Surgery, University of Milano, Milano,...

Ngày tải lên: 25/10/2012, 11:40

... transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD finger- DNA-binding proteins, cysteinyl, lysyl-tRNA syn- thetase), important RNA-stabilising factor - poly (A) binding protein, ... automatically stained with streptavidin-phycoerythrin by using a fluidic station (Affymetrix, USA). Microarrays were scanned by GeneChip Scanner (Affymetrix, USA). The...

Ngày tải lên: 03/11/2012, 10:58

... staining of cystatin F and ER. (A) Cystatin F was stained with a human cystatin F specific polyclonal antiserum and a FITC labelled secondary antibody (green sig- nal). (B) Following the cystatin ... to examine a possible costaining of intracellular cystatin F (Fig. 6A) with Texas Red labelled concanavalin A as an ER-specific marker (Fig. 6B). A colocalization of cystatin F wit...

Ngày tải lên: 21/02/2014, 01:21

... a- L -arabinopyranoside, b- L -arabinopyranoside, a- L -arabino-furanoside, a- D -man- nopyranoside, a- D -mannopyranoside, a- D -fucopyranoside, a- D -xylopyranoside, a- L -rhamnopyranoside) w as deter- mined u sing the ... ietary xenobiotics than f or other aryl-glycosides. These data indicate that human CBG hydrolyses a broad range of dietary glucosides a nd may play a...

Ngày tải lên: 08/03/2014, 16:20

... expression in branchial epithelial cells of Japanese eel, Anguilla japonica Kentaro Miyamoto 1 , Nobuhiro Nakamura 1 , Masahide Kashiwagi 1 , Shinji Honda 1 , Akira Kato 1 , Sanae Hasegawa 2 , Yoshio Takei 2 and ... specimens, anti-eRBCC immunostaining was observed mainly in epithelial cells of the secondary lamella (Fig. 8, panels a and e). The staining pattern was reminis- cento...

Ngày tải lên: 17/03/2014, 10:20

... Biochemistry, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata City, Yamagata 990-9585, Japan. Fax: + 81 23 628 5230, Tel.: + 81 23 628 5227, E-mail: jfujii@med.id.yamagata-u.ac.jp Abbreviations: ... using an NADPH as the electron source. The function of GR in the male genital tract of the rat was examined by meas- uring its enzymatic activity and examining the gen...

Ngày tải lên: 17/03/2014, 17:20

... cpn60; protozoa. Tubulins are highly conserved proteins of eukaryotic cells involved in many essential cellular processes including intracellular transport, cell division and motility. Together with actin ... [8] whereas GroEL has a sevenfold symmetry and is made of a single polypeptide chain. In addition, and in contrast to GroEL, CCT binds and folds a small range of mi...

Ngày tải lên: 23/03/2014, 21:20

... (5¢-GGAGGGTGGCAGCATGTCGTTCCTTGG GG-3¢,forward),GSP 5a( 5¢-GTGGCTTCTTGAGTCAT AGAATCGTGGATGAC-3¢, reverse) and GSP5b (5¢-GT GCATACAACGAAGGCAATCGAGGTCC-3¢, reverse) using Marathon TM cDNA Amplification Kit and Advant- ageÒ cDNA polymerase from ... is also conserved in family 1 of glycosyl hydrolases, although it was shown that only aspartic acid plays an important, but not critical, role in...

Ngày tải lên: 24/03/2014, 03:21

... 1-nitropropane, 2-nitropropane and nitro- ethane catalyzed by NaoA, indicating that NaoA is a type of nitroalkane-oxidizing enzyme. Furthermore, the deduced amino-acid sequence of NaoA has higher ... sulfate fraction; lane 4, recombinant NaoA after Sephadex G75 chromatography; lane 5, purified recombinant NaoA after DEAE-Sepharose Fast Flow chromatography; lane 6, standard molecular ma...

Ngày tải lên: 31/03/2014, 08:20