Báo cáo Y học: Role of histidine 42 in ascorbate peroxidase Kinetic analysis of the H42A and H42E variants pptx

... 5B). Recovery of H42A activity Steady-state and pre-steady-state analyses of H42A indi- cated that the enzyme was essentially inactive. Rate constants for Compound I formation for H42A in the presence of ... clearly active against both L -ascorbate and guaiacol) and has been noted previously for the H42E [49,50] and H42A and H42V [51] variants of HRP. T...

Ngày tải lên: 18/03/2014, 01:20

... fatty acid synthesis in the liver. ACC, the rate-limiting en- zyme that catalyzes the carboxylation of acetyl-Co A to form malonyl-CoA, is the pivotal enzyme in the biosynthesis of long-chain ... ones being lysophosphatidylcholines and lysophosphatidylethanolamines [30]. Soyasapon- ins are one of the major classes of phytochemicals present in soy. The primar...

Ngày tải lên: 31/10/2012, 14:59

... knowledge, the work described here and in the related publications [1,7] is the first kinetic analysis of the in vitro interactions Cyt f–plastocyanin and plastocyanin– Fig. 4. Comparison of ionic ... N-terminal lumenal helix of PsaF has been shown to be involved in binding of plastocyanin [9,10]. In cyanobacteria, deletion of PsaF did not change the kinetic...

Ngày tải lên: 21/02/2014, 01:21

... activity as compared with that of the wild-type [16,17]. We will report here the genetic analysis of hf-2 in detail and the attempt to compensate the deficiency of SQDG in hf-2 by the culture in the ... a conformational change of the PSII complex may cause the decrease of PSII activity in hf-2bythelackofspecific binding of SQDG to the PSII complex. Alte...

Ngày tải lên: 22/02/2014, 04:20

... acid and BPB, the inhibitors of type IB and type V, and BEL, an inhibitor of type VI, inhibited significantly the TG-induced [ 3 H]AA release. The type IV-PLA 2 does not seem to play a role in the ... T-cells constitutively express the mRNA of four PLA 2 isotypes (type IB, type V, type IV and type VI). Interestingly, addition of TG stimulated the induction of...

Ngày tải lên: 08/03/2014, 09:20

... stabilizing the transient semiquinone, destabilizing the flavin hydroquinone complex and therefore in uencing the FAD reduction potential, support the original hypothesis of its role in proton ... water cavity, at the bottom of which are the pyrophosphate and the ribose from the FAD. Therefore, K75 cannot be directly modulating the potential of the flavin ring...

Ngày tải lên: 08/03/2014, 23:20

... from the hydroxyl side chain of tyrosine 229. This displacement may be facilitated involving by R389 through lowering the pK of the tyrosine hydroxyl group. The proton is delivered to the carboxybiotin ... values (Table 1 and Fig. 3). The pH optimum of the enzyme was between pH 6.3 and 6.9. Interestingly, the affinity of the enzyme for Na + increased approximately...

Ngày tải lên: 08/03/2014, 23:20

... similar to those of the wild-type enzyme but the degree of stabilization of the flavin semiquinone and the redox properties in the free form of Y2 38S are different. The binding of the carboxylic acid competitive ... significant increase in the intensity of the Ôcharge transferÕ absorbance band at % 600 nm following the binding of anthranilate (Fig. 3, in...

Ngày tải lên: 17/03/2014, 17:20

... pathogenesis of AC. The findings in our case indicate that early neuroimaging is mandatory in the counterpart of the sympto- matic patient with AC, irrespective of the absence of symptoms. Key words: ... pregnancy of a healthy 24-year-old mother. They have no family history of genetic diseases. Both had Apgar scores of 9/9 at 1 minute and 9/9 at 5 minutes. Th...

Ngày tải lên: 25/10/2012, 11:00

... beads analysis Sample pretreatments and proteomic analysis in the proteomic profiling analysis, the serum samples from the diseased and control groups were rando- mized, and blinded to investigators. ... protein profile of the serum samples from the 98 patients with CRC and the 80 healthy controls were extracted by magnetic beads and examined by MALDI-TOF-MS...

Ngày tải lên: 25/10/2012, 11:18