Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf

... 2002 Functional integration of AACs in E. coli (Eur. J. Biochem. 269) 3181 Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different ... significantly higher affinities for both, ADP and ATP. The corresponding K m values range between 10 and 22 l M . They are only slightly in ue...

Ngày tải lên: 18/03/2014, 01:20

... liver. Furthermore, we investigated the speci®city of the human CBG with r espect to the glycone and aglycone moieties, and in particular characterized the ef®ciency of the enzyme in hydrolysing a ... (1997) The crystal structures of Sinapis alba myro- sinase and a covalent glycosyl±enzyme intermediate provide insights into the substrate recogn ition and active...

Ngày tải lên: 08/03/2014, 16:20

... in the N-terminal region of the Aa-chain by the splitting off of fibrinopeptide A [6]. Site ‘a’ is formed by amino acid residues cGln329, cAsp330, cHis340 and cAsp364, situated in the cC-domain ... Bb14. Turbidity analysis showed an increase in lag-time and a decrease in maximum polymerization rate of this fibrinogen in the desAA fibrin form. The final turbidity o...

Ngày tải lên: 30/03/2014, 03:20

... H abolished the upper band of the anti-VRL-1 immunoreac- tivity doublet band at > 80/84 kDa. The lower band of the doublet at 80 kDa increased proportionally in intensity, but the diffuse ... shown by an deglycosyla- tion assay. Immunofluorescence analysis of the endo- genous VRL-1 in F-11 cells gives only weak signals in the cytoplasm whereas the overexpressed r...

Ngày tải lên: 31/03/2014, 07:20

... found in DM43 first three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using ... a 1 B-glycoprotein, indicating the presence of five immunoglobulin-like domains. DM64 neutralized both the in vivo myotoxicity and the in vitro cytotoxicity of myo...

Ngày tải lên: 21/02/2014, 01:21

... termed the a-crystallin domain, distinguishes a ll small heat shock/a-crystallin proteins, and a two or three domain structure is proposed for these proteins [7,8]. The a-crystallin domain, located toward ... °Cand37°C, although testing at higher temperatures may be informative. Additionally, the C-terminal extension of p26 is required for full induction of thermotolerance in...

Ngày tải lên: 22/02/2014, 04:20

... Groningen, the Netherlands) in order to obtain a recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus. A plasmid containing the wild type version of CCR5 ... membrane proteins allows the study of their behaviour in a membrane in the absence of other proteins, and the manipulation of their concentration and environmen...

Ngày tải lên: 08/03/2014, 09:20

... are involved in the b inding of the UDP-galactose donor, t he residue W315 in the binding of the N-acetylglucosamine-b-p- nitrophenol acceptor, and the domain including E264 appears t o participate in the ... b1,3-N-acetylglucosaminyltransferases, thereby suggesting that they are more likely involved in mediating the b1,3-linkage speci®city r ather than in t he d...

Ngày tải lên: 08/03/2014, 16:20

... located in the EF1, BC2, and FG2 loops and the interdomain segment of c c .The functional b inding epitope of c c includes r esidues I100, L102, Y1 03, and L208 as major binding dete rminants and five ... c c defined in our study also participate in IL-2 and IL-7 binding, as, in the aforementioned s tudy, only one re sidue (Y1 03) was shown to be directly invol...

Ngày tải lên: 08/03/2014, 16:20

... ratio of phycocyanin to phycoerythrin in rods of certain phycobilisomes to improve light harvesting [8]. Furthermore, modulation of the energy levels of the four chemically different bilins by a ... masses of the proteins in each HPLC peak. The peaks eluting within 25 min represented linker p roteins, while the four main peaks between 28 and 33 min were the phyco...

Ngày tải lên: 08/03/2014, 16:20