Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx

... bi[tris(hydroxymethyl)-meth- ylamino] propane and 10 lL10m MD -Ala- D -Ala, incuba- ted at 37 °C for 30 min, and assayed for D , D -dipeptidase activity using the D -amino acid oxidase assay. Kinetic analysis of VanXY C His 6 –VanXY C (2.25 ... proteins eliminate D -Ala-terminating peptidoglycan precursors and replace the terminal D -Ala- D -Ala with D -Ala- D -Ser. In VanA and Va...

Ngày tải lên: 18/03/2014, 01:20

... composed of an N-terminal heavy chain, the bradykinin moiety and a C-terminal light chain. The heavy chain and light chain are interlinked by disulfide bridges. Kininogens are further classified into ... drop- dialysed against water and analysed by MALDI-TOF MS. Phosphatase treatment Dry N-glycan samples were dissolved in 50 m M ammonium bicarbonate, and 1 U of calf intestinal a...

Ngày tải lên: 08/03/2014, 23:20

... nonimmunoglobulin-type carbohydrate recognition molecules that are involved in hemagglutination, lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certain polysaccharides ... buffer containing 0.2 M galactose. Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as th...

Ngày tải lên: 18/03/2014, 01:20

... directly available for plant uptake. A correlation exists between intracellular and/ or extracellular AP activity and cellular P i status [2,7]. An increase in secreted AP and P i -uptake activity ... standard proteins: myosin (200 kDa), b-galactosidase (116 kDa), phosphory- lase B (97.5 kDa), BSA (66 kDa), ovalbumin (45 kDa), and carbonic anhydrase (29 kDa). AP was also visualized...

Ngày tải lên: 23/03/2014, 21:20

... Biosystems). Protein assay and amino-acid analysis Protein concentrations were determined by the Amido black assay [24] using BSA as a standard. For the puri®ed receptor, amino-acid analysis was performed on a ... Biochrom 20 amino-acid analyser (Amersham Pharmacia) af ter hydrolysis in 6 M HCl for 18 h at 110 °C. Comparing results f rom amino-acid ana lysis and A mido black a s...

Ngày tải lên: 24/03/2014, 00:21

... (I in pig and human; L in rat), 680 (L in pig and human; I in rat), 760 (K in pig; R in rat and human), 991 (A in pig and human; S in rat), 1009 (M in pig and human; R in r at) and 1023 (L in ... using a polyclonal antibody against porcine albumin. Lactate dehydrogenase activity in the culture medium was determined by a modified method of Bergmeyer &a...

Ngày tải lên: 31/03/2014, 21:21

... the purification and characterization of chondroitin ABC and AC lyases, particulary chondroitin AC lyase, from an anaerobic bacterium (B. stercoris) in human intestine. The substrate specificity and ... of chondroitin ABC and AC lyase show significantly greater homology of 59 and 80% to Flavobacterial chondroitin AC lyase, and 57 and 33% to B. thetaiotaomicron chondroit...

Ngày tải lên: 31/03/2014, 23:20

... showed that they hold the characteristic features of all known papain-class cysteine proteinases, and a phylogenetic analysis revealed the existence of several papain and chymopapain homologues in ... proteinases it can be deduced that the common ancestor of the genera Carica and Vasconcellea already contained at least two different cysteine pro- teinases in its latex (papai...

Ngày tải lên: 07/03/2014, 11:20

... citrulline and arginine biosynthesis. AGS, N-acetylglutamate synthase; AGK, N-acetylglutamate kinase; AGPR, N-acetylglutamate 5-phosphate reductase; AOAT, N-acetylornithine transaminase; GAT, glutamate ... glutamate N-acetyltransferase; AOD, N-acetyl- ornithine deacetylase; OCT, ornithine carbamoyltransferase; ASS, argininosuccinate synthase and ASL, argininosuccinate lyase. Wild watermel...

Ngày tải lên: 20/02/2014, 03:20

... Bovine submaxillary mucin, which contains mainly 9-O-acetyl- and 8,9 di-O-acety-N- acetyl neuraminic acid was the most potent inhibitor of the lectin. Sialidase treatment and de-O-acetylation of ... glycosidic linkages [5] on cell-surface glycocon- jugates, namely glycoproteins and glycolipids, or in bacter- ial polysaccharides. Sialic acids are a family of sugars, N-acetylneu...

Ngày tải lên: 21/02/2014, 00:20