Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp ) ppt

... Purification and characterization of three galactose specific lectins from Mulberry seeds ( Morus sp. ) Tanzima Yeasmin, Md Abul Kashem Tang, Abdur Razzaque and Nurul Absar Department of Biochemistry, ... characterization of three galactose- specific lectins from the seeds of mulberry. MATERIALS AND METHODS Mulberry seeds were collected fr...

Ngày tải lên: 18/03/2014, 01:20

... triantennary species, respect- ively (Fig. 4B). Sulfated species are observed also for the monosialylated biantennary and disialylated triantennary species, at m/z 2012.2 and m/z 2667.6, respectively. To ... Kalkkinen 1 1 Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland, 2 Norwegian College of Fishery Science, University of Tromsø, Norway,...

Ngày tải lên: 08/03/2014, 23:20

... specific- ally hydrolyse D -Ala- D -Ala with minimal activity against D -Ala- D -Ser, a very different type of specificity. VanXY C , VanX-type and VanY-type enzymes contain most of the active site ... SDS/PAGE analysis. (A) Purification of VanXY C .Lane1, molecular mass standards (in kDa); Lane 2, partially pure VanXY C after gel filtration chromatography. (B) Purification of MBP-...

Ngày tải lên: 18/03/2014, 01:20

... to identify the physiological electron acceptor of the enzyme. The enzyme was not oxidized by the heterodisulfide of coenzyme M and coenzyme B, or by the homodisulfides of these two coenzymes. Also ... ¢ ¼ )1 45 mV), anthra- quinone-1,4-disulfonate (E ¢ ¼ )1 70 mV), phenosafranin (E ¢ ¼ )2 52 mV), anthraquinone-2-sulfonate ( E°¢¼ )2 55 mV), safranin O (E ¢ ¼ )2 89 mV), a...

Ngày tải lên: 21/02/2014, 03:20

... completely conserved (Cys14–Cys43, Cys26– Cys71, Cys62–Cys128, Cys63–Cys67, Cys100–Cys132 and Cys108–Cys119 in human mature cathepsin B). Residues in the S1, S2 and S1¢ sites (S1, Gln23 and Gly74; ... kDa), phosphorylase b (97 kDa), BSA (67 kDa), ovalbumin (45 kDa), glyc- eraldehyde-3-phosphate dehydrogenase (36 kDa), trypsin inhibitor (20 kDa) and egg cystatin (12.8 kDa). (C) Similar...

Ngày tải lên: 16/03/2014, 04:20

... sequencing and MALDI-TOF MS analy- sis. This work was supported partly by a Creative Scientific Research from the Japan Society for the Pro- motion of Science (JSPS-13GS002 3) and partly by an Intellectual ... SDS ⁄ PAGE of GAT. The peak fraction obtained after hydroxylapatite chromatography was analysed by PAGE (12% w ⁄ v, polyaclylamide) and detected by silver staining. (B) Mo...

Ngày tải lên: 20/02/2014, 03:20

... at molecular mass of 34 kDa (Fig. 2). Erythrocyte-binding specificity of P. jacquemontii lectin The Paratelphusa lectin agglutinated only a limited range of erythrocytes. Out of 12 erythrocyte types tested ... lectin from Scylla serrata was NeuGc specific [24] and that from Cancer antennarius was 9-O-NeuAc and 4-O-NeuAc specific [12,28]. Lectins with defined specificity fo...

Ngày tải lên: 21/02/2014, 00:20

... obtained from Promega, and LysC of Achromobacter lytii from Wako. The O-phosphorylated phosphopeptides EQTRsLDGR-NH 2 , DHTGFLtEY- VATRWC-NH 2 , DADEyL-NH 2 , EGDNDyIIPL-NH 2 , KKPRyEIRW-NH 2 , and ... APDyTTPEMY-NH 2 were obtained from Dr A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsa...

Ngày tải lên: 21/02/2014, 01:21

... homologies with UspA. Two of them, encoded by ybdQ and ynaF, were previously identified as unknown proteins (UP12 and UP03, respectively) by 2D-PAGE [16]. Three E. coli paralogues of UspA have been ... and expression of UP12 (j). (B) Monitoring of cell growth (s )and amount of UP12 (d) before and after addition of 0.1 m M CCCP. (C) Effect of temperature on cell growth (...

Ngày tải lên: 22/02/2014, 07:20

... molecular phylogenetic analysis of fish hatching enzyme genes, hatching enzyme originally consisted of a single type of enzyme, and HCE and LCE were produced by duplication and diversification of the ... (Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo) for reading the manuscript and to Dr K. Yamagami (former Profess...

Ngày tải lên: 07/03/2014, 04:20