Báo cáo Y học: Identification of mammalian-type transglutaminase in Physarum polycephalum Evidence from the cDNA sequence and involvement of GTP in the regulation of transamidating activity potx
Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... monomer, as was the case also with the purified porcine enzyme. The nucleotide sequence of the cloned human cDNA and the corresponding amino acid sequence are shown in Fig. 1. The identity of the expressed protein ... peptides obtained by digestion of the purified porcine enzyme with trypsin or LysC. The resulting peptide sequences are shown in Table 2. Using...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... structures found in hygromycin A from Streptomyces hygroscopicus [3,4]. These similarities suggest that certain enzymes, and therefore the corresponding genes of the A201A biosyn- thetic pathway, may be ... be related to their counterparts of the puromycin and hygromycin A biosynthetic pathways, respectively. The puromycin biosynthetic gene cluster (pur )from S. alboniger...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... s erine/threonine protein k inases widely expressed in a range of eukaryotes including yeast, mammals and plants. They have been shown to play a role in diverse physiological events including ... CKI may play a role in synaptic vesicle exocytosis. Keywords: CKI; CKII; syntaxin-1A; trafficking. Casein kinase I (CKI) belongs to a family of serine/ threonine protein kinases with seven...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... UspA domains in tandem [17]. The small members of this family are proteins of 142–144 amino-acids long; they are acidic and presumably located in the cytoplasm like UspA. Members of this family share ... extracts using anti-GroEL Ig. It has been previously shown that at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases con...
Ngày tải lên: 22/02/2014, 07:20
Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... Rpn8 and Rpn11), and described the relationship between N-acetylation and the chymotrypsin-like activity of 20S proteasome. In the present s tudy, we detec ted the N-acetylation of OsRpt6 in the ... 1). On the o ther hand, in our study, the full length of homologs of ScRpt4 and ScRpt6 were cloned by 5¢ RACE using C 50126 and R1494 as primers from the...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... possibly a metal- dependent enzyme. Substrate specificity analysis To study the substrate specificity of the N-D-AAase protein, the activity of the enzyme against N-acyl- D -or L -amino acids and other D -amino ... of hydrolysis for gelatin and starch. Therefore, according to all above analysis results, the strain Iso1 was clearly a strain of V. paradoxus. Cloning and...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
... where the basal activity increased dramatically, and the mouse Q404H mutant, where the basal activity decreased by more than 50% (Fig. 4). The basal activity of H407Q and Q404A on the other hand ... of the human and mouse PXR ligand binding domains. By modeling the human PXR ligand binding domain we have identified and mutated two polar residues in the puta-...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx
... C a of Glu and its carboxylate oxygen, C a of Asp a nd its carboxylate oxygen, C a of Arg and its side-chain amines or guanidinium, and C a of Lys and its side-chain amine was determined by measuring ... the active site by the interaction of t he N-terminal amine of t he P1 residue with a c arboxylate oxygen of Asp273 (Asp366), the carbonyl oxygen of the P1 res...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Identification of novel membrane proteins by searching for patterns in hydropathy profiles potx
... pattern of peaks in their hydropathy profiles. (A) The hydropathy profile of a rat dopamine symporter reveals a pair of peaks followed by a deep valley, then a cluster of nine peaks. The peak, base and ... designed for the acetylcholine (ACh) and glycine receptors of the cys-loop receptor superfamily. The key feature was a closely spaced triplet of hydropathy peaks b...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo Y học: Identification of mammalian-type transglutaminase in Physarum polycephalum Evidence from the cDNA sequence and involvement of GTP in the regulation of transamidating activity potx
... suggest that the mechanism by which nucleotides inhibit the enzymatic activity of PpTGase might be similar to that by which they inhibit the enzymatic activity of TGase 4. Hydrolysing activity of GTP ... of mammalian-type transglutaminase in Physarum polycephalum Evidence from the cDNA sequence and involvement of GTP in the regulation o...
Ngày tải lên: 17/03/2014, 23:20