... 4).Similarly, Shaanan [31] reported the stereochemistry of the iron-dioxygen bond in human HbO2by single-crystalX-ray analysis. In the a chain, the distance between Ne of His (E7) and the terminal ... valencyhybrids; a1b1 contact; a cid-catalysis. The reversible and stable binding of molecular oxygen to the heme iron(II) is the b asis of hemoglobin function. However, the oxygenated form of hemoglobin, ... experimentalconditions, the valency hybrid hemoglobins were suf®cientlystable for the rate measurements over a long period of time.In separated a and b chains, on the other hand, the addition of 20% (v/v) glycerol...