... the activity of the enzyme was measured by the dinitrosalicylicacid method.Inhibition of the enzyme activity by glucose, maltose andcyclohexaamyloseFor the study of the enzyme inhibition by glucose, ... enzymewas determined using the Betamyl b-amylase test reagent(Megazyme, Wicklow, Ireland).To determine the pH stability of the b-amylase, aliquots of a solution of the purified protein (4.06 mg·mL21 in ... of the b-amylase from hedge bindweed and the model b-amylase from soybean. HCA plots were generated using the programHCA-Plot2 (Doriane, Paris, France).Molecular modeling of the b-amylase from...