Báo cáo Y học: The role of zinc in the methylation of the coenzyme M thiol group in methanol:coenzyme M methyltransferase from Methanosarcina barkeri New insights from X-ray absorption spectroscopy doc
Báo cáo y học: "Why are some children with early onset of asthma getting better over the years" -aneuploid-prostate-cancer-cells-after-tmz-tmz-bioshuttle-treatment.html#post143974
... University Hospital, including PHC data of the statements made by the mid-wife in her records of the check-up of the mothers during the pregnancy (17). In the follow-up at the age of seven, ... asthma remain asymptomatic without needing ther- apy for the rest of their lives, but asthma remission also does exist (12). The more severe the asthma is in c...
Ngày tải lên: 26/10/2012, 09:48
Báo cáo y học: "Grb2-associated binder 1 polymorphism was associated with the risk of Helicobactor pylori infection and gastric atrophy"
... The Gab1 polymorphism may affect the interaction with SHP-2 through the mechanism such as BTAM after H pylori infection, resulting in influencing the abnormal proliferation and movement of ... domains or by specific phosphopeptides that bind to the SH2 domains. The tandem SH2 domains bind to Tyr-627 and Tyr-659 simultaneously in a specific orientation, in which Tyr-62...
Ngày tải lên: 31/10/2012, 15:37
Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... the activity of the enzyme was measured by the dinitrosalicylic acid method. Inhibition of the enzyme activity by glucose, maltose and cyclohexaamylose For the study of the enzyme inhibition by glucose, ... enzyme was determined using the Betamyl b-amylase test reagent (Megazyme, Wicklow, Ireland). To determine the pH stability of the b-amylase, aliquots of a solut...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... between the buffer and the enzyme by preincubating the enzyme–metal mixture at 30 8C for 30 min. Remaining activity was measured with glucose as the substrate in the presence of 4 m M CoCl 2 . The apoenzyme ... metal-containing enzymes, the apoenzyme was incubated for 2 h at room temperature with 5 m M metal chloride. The enzyme solution was then dialyzed once against...
Ngày tải lên: 22/02/2014, 07:20
Báo cáo khoa học: A new rice zinc-finger protein binds to the O2S box of the a-amylase gene promoter pptx
... data indicate that RAMY protein binds specifically to the O2S elemen t. We have also determined the importance of the amino acids within the binding domain of RAMY protein and analyzed the time ... GST-mutant RAMY fusion protein (lanes 3–6), showing RAMY binding to DNA a nd the effects of mutations within the RAMY DNA-binding domain on the ability of the purified fusion...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo Y học: Cellular stresses profoundly inhibit protein synthesis and modulate the states of phosphorylation of multiple translation factors potx
... translation in mammalian cells involves the regulation of a range of components of the translational machinery, principally by changes in their phosphorylation, leading to modulation of their activities ... SDS/polyacrylamide gels containing 15% acryla- mide/0.4% methylene bis-acrylamide. For analysis of eEF2, gels contained 10% (w/v) acrylamide plus 0.1% methylene bis-acryl...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo Y học: Extra terminal residues have a profound effect on the folding and solubility of a Plasmodium falciparum sexual stage-specific protein over-expressed in Escherichia coli pptx
... proper folding or of increased solubility of the protein of interest. We propose that the success of fusion protein systems should be ascertained only once the protein of interest has been cleaved off ... that the fusion proteins were labile and only partially soluble in five of the constructs resulting in very poor yields. Intriguingly, in the sixth con- struct,...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot
... a 2 b 2 c 2 composition. The K m for the three main substrates were 1.6 m M for 1,2-propanediol, 5.5 m M for 1,2-ethanediol and 8.3 m M for glycerol. The enzyme required the adenosylcobalamin coenzyme for catalytic activity ... 5735 metabolism is catalysed by a dehydratase that converts glycerol to 3-hydroxypropionaldehyde, a precursor of acrolein [12]. The dehydratase e...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo Y học: Amphibian peptides that inhibit neuronal nitric oxide synthase The isolation of lesueurin from the skin secretion of the Australian Stony Creek Frog Litoria lesueuri docx
... tructure of the calmodulin binding sites of any isoform being reported. Thus, we cannot make meaningful comparisons of common structural motifs that might underlie calmodulin binding to the e nzymes. Most ... further examine t he mode of action by which they were inhibiting nNOS. Lesueurin and citro- pin 1.1 (both from inhibitor group 1), f renatin 3 (inhibitor group 2) and...
Ngày tải lên: 31/03/2014, 15:20
Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
... [49,50]. Interaction of the YopD peptide with LcrH In the yeast two hybrid assay, the C-terminal amphi- pathic domain of YopD was required for LcrH binding [13]. Because the YopD–LcrH complex is important ... domain of YopD. The peptide structures were examined using CD spectroscopy and 2D homonuclear/heteronuclear NMR spectroscopy. Using these peptides, the interactio...
Ngày tải lên: 31/03/2014, 21:21