Báo cáo Y học: Regulation of stress-activated protein kinase signaling pathways by protein phosphatases pot

... at least in part, by G -protein coupled receptors (GPCRs), stimulation of membrane phospholipid hydrolysis, and activation of the diacylglycerol sensitive isoforms of protein kinase C (PKC) [28]. ... the activation of ERK by GPCRs closely parallels that employed by RTK [33]. On the whole, these observations suggest that the inhibitory effect elicited by NAMI-A on ERK sig...

Ngày tải lên: 21/02/2014, 01:21

... in the regulation of yeast SAPK pathways, then fo cus on the properties of the protein phosphatases implicated in the mammalian SAPK systems. REGULATION OF SAPK SIGNAL PATHWAYS BY PROTEIN PHOSPHATASES ... MINIREVIEW Regulation of stress-activated protein kinase signaling pathways by protein phosphatases Shinri Tamura, Masahito Hanada, Motoko Ohnishi,...

Ngày tải lên: 17/03/2014, 23:20

... exclusively but, rather, by a set of different proteins that are structurally related [1]. The promoter regions of many eukaryotic genes contain GC-rich sequences [2] and some of the most widely distributed ... identification by Bigey et al.ofmany Correspondence to K. K. Yokoyama, Gene Engineering Division, Department of Biological Systems, BioResource Center, Tsukuba Institute, RI...

Ngày tải lên: 22/02/2014, 07:20

... Double-positive clones were purified by secondary and tertiary r ounds of screening and genomic DNA inserts were purified using poly(ethylene glycol) precipitation [26]. The phage inserts were mapped by restriction ... s described by Koike et al. [21] are controlled by still another regulatory sequence, as they initiate several hundreds of bp upstream of the start of transcript...

Ngày tải lên: 08/03/2014, 16:20

... GPVI, glycoprotein VI; PtdIns3K, phosphatidylinositol 3 -kinase; PVDF, poly(vinylidene difluoride); PKC, protein kinase C; PP1, (4-amino-4-(4-methylphe- nyl)-7-(t-butyl)pyrazola[3,4-d]pyrimidine); ... ere lysed in Laemmli sa mple buffer a nd were resolved by 12% SDS/ PAGE. Phosphorylated pleckstrin w as detected by autoradiography. (B) Platelets were lysed by the addition of denatur...

Ngày tải lên: 08/03/2014, 16:20

... glycosylphophatidylinositol (GPI) anchors [1,2]. Although the primary structure of the glypi- cans is only marginally conserved (about 30% identity), there is a strict conservation of 14 cysteine ... patterns [6–10]. Modifications in glypican and syndecan expression may be induced by activation of signaling processes. It was shown that glypican-1 is downregulated by the presence o...

Ngày tải lên: 17/03/2014, 23:20

... Denmark Synthesis of dTMP by thymidylate synthase proceeds by the reductive methylation of dUMP, which is obtained via one of two parallel pathways. One path- way, considered to be a minor supplier of ... donkey deoxycytidylate aminohydrolase (EC 3.5.4.12). Arch Biochem Biophys 289, 19–25. 5 Ellims PH, Kao AY & Chabner BA (1983) Kinetic behaviour and allosteric regulation...

Ngày tải lên: 18/02/2014, 16:20

... ith a specific enz yme activity. Keywords: Bombyx mori; detoxication; insect; UDP- glycosyltransferase. The UDP-glycosyltransferases ( UGTs) are a superfamily of enzymes that p lay a central role ... UDP-glucosyltransferase gene, Bmugt1,and analysis of the activity of the enzyme towards a range of Correspondence to D. R. O’Reilly, Department of Biology, Imperial College of S cience...

Ngày tải lên: 22/02/2014, 04:20

... Inhibition of the NF-jB transcriptional activity by protein kinase A Naoko Takahashi, Toshifumi Tetsuka, Hiroaki Uranishi and Takashi Okamoto Department of Molecular Genetics, Nagoya City University Medical ... transfection assays are corrected by the internal control activity of Renilla luciferase by pRL-TK. Electrophoretic mobility shift assay (EMSA) Jurkat T-lymphocytes wer...

Ngày tải lên: 08/03/2014, 10:20

... double bonds by so-called (1,4)-acyl-lipid-desaturases (FADX) this class of enzymes catalyzes the formation of hydroxy, epoxy, and acetylenic groups, respectively, within a fatty acid backbone ... Saccharomyces cerevisiae, and formation of newly formed fatty acids was analyzed by gas chromatography-free induction decay (GC-FID) and GC/MS. The expression of the heterologous enzymes...

Ngày tải lên: 08/03/2014, 16:20