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Báo cáo khoa học: "The GENIA project: corpus-based knowledge acquisition and information extraction from genome research papers" docx

Báo cáo khoa học:

Báo cáo khoa học: "The GENIA project: corpus-based knowledge acquisition and information extraction from genome research papers" docx

... Proceedings of EACL '99 The GENIA project: corpus-based knowledge acquisition and information extraction from genome research papers Nigel Collier, Hyun Seok Park, Norihiro ... biochemical information from journal papers and ab- stracts. GENIA will be available over the Internet and is designed to aid in information extraction, retrieval and vi- sualisation and to help ... global research effort to map the human genome, the Genome Informatics Ex- traction project, GENIA (GENIA, 1999), aims to support such research by automatically extract- ing information from...
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Báo cáo khoa học:

Báo cáo khoa học: "Flow Network Models for Word Alignment and Terminology Extraction from Bilingual Corpora" docx

... tween bandwidth and largeur de bande can be obtained through the edge linking these two units (type 1), or through two edges, one from bandwidth to largeur de bande., and one from bandwidth ... edges from bandwidth to largeur and bande (type 3), 2. secondly, there may be conflicts between connections: in figure 1 both largeur de bande and tdldcommunications are linked to bandwidth ... multilingual terminology extraction. 3 Multilingual terminology extraction Several works describe methods to extract terms, or candidate terms, in English and/ or French (Justeson and Katz, 1995;...
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Tài liệu Báo cáo khóa học: The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life pptx

Tài liệu Báo cáo khóa học: The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life pptx

... lCiÆmL)1,160mCiÆmmol)1)wasfromPerkinElmerLife Sciences Inc. Restriction enzymes were from NewEngland Biolabs. Expand High Fidelity polymerase and inorganic pyrophosphatase were from Roche. Nucleotri-phosphates ... SerRS forms complexes with homologous and heterologous archaeal tRNAsSer and tRNAsSec(lanes 3 and 4, 5 and 6, respectively) as well as with tRNASersubstrates from other domains of life (E. coli,lane7;yeast,lane ... chromatography; lanes 2 and 3, peak I fractions; lanes 4 and 5,peak II fractions; lanes 6 and 7, peak III fractions. Molecular masses of159 kDa, 66 kDa and 32 kDa, corresponding to peaks 1, II and III,respectively,...
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Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot

Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot

... Primers 1 and 3, primers 2 and 3, and primers 1 and 4 were used to amplify the genes encoding Tma-RNaseHI, Tma-CD and Tma-ND, respectively. The resultantDNA fragments were digested with NdeI and ... analysis.AcknowledgementsThis work was supported in part by a Grant(21380065) from the Ministry of Education, Culture,Sports, Science, and Technology of Japan, and by anIndustrial Technology Research ... athree-stranded anti-parallel b-sheet (b1–b3) and twohelices (aA and aB) [22]. It binds to the minor grooveof the substrate, such that a loop between aA and b3interacts with the RNA backbone and...
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Báo cáo khoa học: The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba docx

Báo cáo khoa học: The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba docx

... distribution of theenzyme and substrates.Deduced from the crystal structure of pyridoxal kin-ase from sheep [17], the hydrophobic and hydrophilicregions of the substrate and cofactor binding domainof ... solution and a 0.1 m ATP stock solu-tion. The general incubation temperature was 37 °C. Todetermine the temperature and pH optima, reactions wereperformed in a range from 20 to 60 °C and from ... Formation of pyridoxal 5¢-phosphate and ginkgotoxin phosphate (MPNP) during incubation of pyridoxal kinase separately (A) and simul-taneously (B,C) with pyridoxal and ginkgotoxin. (A,B) PL ¼ 0.025...
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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

... related toPAL2, and PAL3 always clusters together with PAL4[61]. In A. thaliana, from the molecular phenotype,common and specific functions of PAL1 and PAL2were delineated, and PAL1 was qualified ... 266–295 (chain B) and 385–399 (chain C) inSmPALout ⁄ in and 33–128 and 333–351 (chain A), 272–303(chain B) and 293–306 (chain C) in PlPALout ⁄ in. The preop-timizations and molecular dynamics ... prepa-rations from plants, e.g. parsley [66], potato tubers[12], maize shoots [67], gherkin [68] and wheat seed-lings [69], showed significant deviations from Michae-lis-Menten kinetics. On the other hand,...
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Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx

Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx

... toMBP were from New England Biolabs. Polyclonal anti-bodies to the N-terminal region of RasGRF1 have beenpreviously described [28]. Chemicals were from Sigmaunless otherwise indicated.AcknowledgementsWe ... recep-tors [20,21] and requires both calcium and calmodulinto exert its activity on Ras [18,21]. The protein is phos-phorylated on serine, threonine and tyrosine residuesin vivo and is a substrate ... 6). Supernatants and pellets were isolated by centrifugation, and equal volumes were used for SDS ⁄ PAGE and analyzed by immuno-blotting using monoclonal antibodies to a-tubulin and RasGRF1.Lane...
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Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot

Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot

... RNase H activity are ubiquitous and havebeen isolated from a variety of organisms, ranging from viruses to prokaryotes and eukaryotes [3]. The best char-acterized and f unctionally understood ... lLRNasin(20UálL)1;Promega) and 5 lL cell extract, and incubated at 37 °Cfor5 m in. RNA was subsequently precipitated in the presenceof glycogen, after removal of proteins by phenol extraction, and dissolved ... response to ODNtreatment varied considerably. 15PC3 and M iaPacaIIshowed a good response, while HEK293 and HTB82 hardlyresponded at all, and HeLa and T24 showed an intermediateresponse. To investigate...
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Báo cáo khoa học: The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx

Báo cáo khoa học: The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx

... ranging from 2.0 to 3.0 and from 1.4 to 1.8 with NADH and succinate, respectively,used as substrates.In a set of experiments, Rf (0.2–30 lm) was addedto purified intact TBY-2 mitochondria, and ... mitochondria from mammals and yeasts [29–32]. Moreover, the data here reported implythe existence of intramitochondrial enzymes that allowfor FMN and FAD synthesis starting from exogenousRf and endogenous ... (0.65 m mannitol and 25 mmTris ⁄ Mes, pH 5.5) and treated with Caylase (Cayla, Tou-lose, France) and pectinase (Sigma-Aldrich), as described in[13]. Intact purified mitochondria and plastids wereobtained...
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Báo cáo khoa học: The viral SV40 T antigen cooperates with dj2 to enhance hsc70 chaperone function docx

Báo cáo khoa học: The viral SV40 T antigen cooperates with dj2 to enhance hsc70 chaperone function docx

... subcloned from pSG5-T-H42Q (a gener-ous gift from J. A. DeCaprio) to pGEM and subsequentlyinserted to the EcoR1 and Sal1 sites of pGEX-4T-1. Theresulting plasmid was digested with Not1 and HindIII,blunt ... DnaJs lack the cysteinerepeats and type III DnaJs lack both the G ⁄ F region and the cysteine repeats. Type I and Type II proteinsseem to have similar functions and they bind non-native substrates, ... homologue ofthe bacterial DnaJ and the yeast Ydj1 [10–12]. It ismainly cytosolic and acts as a cochaperone to hsc70assisting the folding of denatured proteins and facilitat-ing the protein...
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