... lCiÆmL)1,160mCiÆmmol)1)wasfromPerkinElmerLife Sciences Inc. Restriction enzymes were from NewEngland Biolabs. Expand High Fidelity polymerase and inorganic pyrophosphatase were from Roche. Nucleotri-phosphates ... SerRS forms complexes with homologous and heterologous archaeal tRNAsSer and tRNAsSec(lanes 3 and 4, 5 and 6, respectively) as well as with tRNASersubstrates from other domains of life (E. coli,lane7;yeast,lane ... chromatography; lanes 2 and 3, peak I fractions; lanes 4 and 5,peak II fractions; lanes 6 and 7, peak III fractions. Molecular masses of159 kDa, 66 kDa and 32 kDa, corresponding to peaks 1, II and III,respectively,...