... quantified by scintillation counting. Preparative aminoacylation reactions,scaled to 100 lL, were allowed to reach a plateau, rapidlyextracted with phenol, and the aminoacylated tRNA wascollected by ... link under the acidic conditions of nuclease treat-ment was confirmed by separate experiments. Radioactivitywas detected by phosphorimager analysis (PharosFXPlus; Bio-Rad, Munich, Germany), ... Fersht AR & Kaethner MM (1976) Enzyme hyperspeci-ficity. Rejection of threonine by the valyl-tRNA synthe-tase by misacylation and hydrolytic editing.Biochemistry 15, 3342–3346.7 von der Haar...