... NC, USA4 Biomedical Research Centre, School of Biological Sciences, University of East Anglia, Norwich, UK5 Department of Neurobiology, University of Pittsburgh, PA, USABasement membranes (BMs) ... biophysical properties of BMs.The lack of data is probably due to the difficulty of obtaining BM preparations that are free of adjacentinterstitial connective tissue and the lack of a suitablemeasuring ... ILM(Fig. 4B), suggesting modification of the BM duringdevelopment.AFM measurement of ILM thicknessThe thickness of ILM was obtained from AFM images of the sharp edges of ILM where the underlining glasssubstrate...