... of dietary curcumin, capsaicin
and their combination on iron-induced LDL
oxidation in vivo and copper-induced LDL
oxidation in vitro
Oxidation of LDL observed in iron(II) sulfate-injected
rats ... Dietary
curcumin and dietary capsaicin significantly inhibited
the oxidation of LDL, as indicated by TBARS values
which were 71 and 62% of control rats. Extent of
iron-induced o...
... interacts with N10 and E97 of the second
subunit. The backbone NH group of R98 forms a
hydrogen bond with the backbone CO of F102. Fur-
thermore, the orientation of the side chain of the two
residues ... nm.
Excitation and emission band passes were set at 5 nm.
Denaturation studies were performed by incubating 10 lm
protein with various concentrations of urea and guanidinium...
... [52]. The details of cDNAs, restriction
sites used and plasmid designations are listed in Table 1.
Subcellular fractionation and isolation of
mitochondria and mitoplasts
Mitochondria were isolated ... mitochondria
with SEM buffer containing phenylmethanesulfonyl fluo-
ride.
Digitonin fractionation
Isolated mitochondria (100 lg) were resuspended in SEM
buffer containing indicated conc...
... post-transfection, clones were picked and expanded. After
induction with doxycycline (1 lgÆmL
)1
), the individual
clones were tested for their expression of the protein of
interest.
Flag ... R, Thomson
CT, Sacchettini JC & DePinho RA (1994) Evolutionary
relationships and functional conservation among verte-
brate Max-associated proteins: the zebra fish homolog
of Mxi1. Oncogen...
... Rochat
H, Harvey AL, Montenay-Garestier T & Menez A
(1990) Delineation of the functional site of a snake
venom cardiotoxin: preparation, structure, and function
of monoacetylated derivatives. ... the
membrane of cells.
Inhibitory effects of VVA1 on the hemolytic and
cytotoxic activity of VVA2
The effects of VVA1 on the hemolytic activity of
VVA2 were studied by inc...
... range of structural diversity produced by a
sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-
tial origin of structural diversity of skeletons in their biosynthesis, and ... soyasapogenol B, and
the presence of additional hydroxylases must be
considered. Fortunately, the aglycone of the major
soyasaponins is soyasapogenol B, and glycosides of...
... allows study of the effects of
replacement of muscle exons 1a and 2a by nonmuscle
exon 1b, and comparison of Tm5a with Tm5b allows
the effects of exchange of exon 6 to be studied.
Studies of thermal ... cooperative
thermal transitions measured. On the other hand, both DSC and CD stud-
ies show that replacement of muscle exons 1a and 2a by nonmuscle exon
1b not only i...
... addition
of 100 lLof2· Kirby buffer [25] and 80 lLofH
2
O, and
rRNA was obtained by precipitation after two phenol–
chloroform extractions. Four micrograms of rRNA were
treatedwith20lL of acetic-aniline ... Electron density of RTA N122A in the vicinity of residue 122. The backbone and sidechains of the N122A substitution are shown as stereo
images in thick ball and stick...
... inhibitor concentration, K
m
is the Michaelis–
Menten constant for the enzyme-substrate pair, S
o
is the
substrate concentration and k
ass
and k
diss
are the associ-
ation and dissociation rate constants, ... well
as their intracellular localization in promonocyte
U937 cells, using specific antibodies and confocal
microscopy.
Results
Activation of cystatin F
Recombinant cystatin F...