... and R I data by using theASTRAsoftware(version 4.75 , WTC; e xtrapolation by D ebye, first order) .Maltooligosaccharide patternsBacterial cells (100 mL suspension) were p ellete d by centrifugation ... chromatography(RP-HPLC)The peptides generated by either in-ge l trypsination or by cyanogen bromide cleavage and subsequent a-amylasetreatment were separated by RP-HPLC (SMART system,Pharmacia, Uppsala, ... Subse-quently, the nucleases were inactivated by heating (5 min at100 °C) and the denatured p rotein was removed by centrif-ugation. The supernatant was concentrated by filtrationusing an Amicon filter...