... opaenke@uos.deAbbreviations:FO, ion-driven rotary motor of F-ATP synthase; F1,rotary chemical reactor of F-ATP synthase; AMP-PNP, 5¢-adenylyl-imidodiphosphate; DTNB, 5,5¢-dithiobis(2-nitrobenzoic acid);TMR-ITC, tetramethyl ... thea-helix in the C-terminal portion of subunit c [20].MM10 served as source for nonfluorescent ac hetero-dimers. For the incorporation of fluorescent a subunits intothe two nonfluorescent a positions ... residues from the N-end of csubunit (cA1–cK24) and 43 residues from its C-end (cT230–cL272). The chosen portion included a major part of thecoiled-coil region of c and the complete a-helical C-terminusas...