Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
... serine in the enzyme from the yeast Rhodo-
torula gracilis. The mutated proteins are catalytically
competent thus eliminating Tyr238 as an active- site acid/
base catalyst. Y2 38F and Y2 38S mutants ... 421506,
E-mail: loredano.pollegioni@uninsubria.it
Abbreviations:DAAO,
D
-amino acid oxidase; RgDAAO, Rhodotorula
gracilis
D
-amino acid oxidase; pkDAAO, pig kidney
D...
Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1 ppt
... 5¢-GAAGTTAAAGATACAATGATTCAGCTC 5¢-GAGCTGAATCATTGTAACTTTAACTTC-3¢ 48
N302D 5¢-CATTGTTGAAGACATCAATGTTG-3¢ 5¢-CAACATTGATGTCTTCAACAATG-3¢ 43
N302F 5¢-CTTTACATTGTTGAATTCATCAATGTTGCAGC-3¢ 5¢-GCTGCAACATTGATGAATTCAACAATGTAAAG-3¢ ... 5¢-CAACATTCCTTGTCATCGAACCAAACTC-3¢ 58
R103M 5¢-GTTCGAGAACAATGAATGTTGTGTTC-3¢ 5¢-GAACACAACATTCATTGTTCTCGAAC-3¢ 55
R103E 5¢-GAACACAACATTCTCTGTTCTCGAACC-3¢ 5¢-GGTTCGAGAACAGA...
Báo cáo khoa học: Post-translational modifications in the active site region of methyl-coenzyme M reductase from methanogenic and methanotrophic archaea potx
... M, and the partial hydrolysate was analyzed by MALDI-TOF
MS. From the peptide ladder, the N-terminal amino acid sequence was found to be LGFY-thioglycine-YALQD. The mass peaks labeled with
an asterisk ... primarily with unreactive aromatic
and aliphatic amino acid residues. The methyl groups
of S-methylcysteine, 5-methylarginine and 2-methylglu-
tamine are oriented towards th...
Báo cáo y học: "Complications after spacer implantation in the treatment of hip joint infections"
... Isiklar et al.
found one case of ARF out of 10 patients after im-
plantation of a vancomycin-loaded hip spacer (2-3 g
vancomycin / 40 g PMMA) and intravenous admini-
stration of the same antibiotic ...
the antibiogram of the causative bacterium and does
not endanger the infection sanitation. Careful and
frequent monitoring of the laboratory parameters are
indicate...
Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... PKC A5 89G_G59 0A_ G591C
F:5'-aaaacaacctcttacccaacaccagacccctatccaaaacctgca-3'
R:5'-tgcaggttttggataggggtctggtgttgggtaagaggttgtttt-3'
Figure 2. Site- directed mutagenesis ...
94
aggressive melanomas have an abundance of
claudin-1 in the cytoplasm. We have shown that in-
creasing claudin-1 expression in less metastatic
melanoma cells caused an inc...
Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... the
glutamate that acts as the catalytic acid. The active site
grooves of these chitinases are lined with aromatic amino
acids that contribute to substrate binding [6,11].
Catalysis in retaining glycoside ... Family 18 chitinases are retaining glycoside
hydrolases that have been found in many organisms varying
from bacteria to humans [1,2]. The catalytic domains of
family...
Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt
... Kinetic and biochemical analyses on the reaction mechanism
of a bacterial ATP-citrate lyase
Tadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi and Tadayuki Imanaka
Department of Synthetic ... cells, as it is
presumed to play a vital role in providing acetyl-CoA and
oxaloacetate in the cytosol as starting materials for a variety
of biosynthetic pathways. Rat and human ACLs f...
Báo cáo Y học: Thermolysin-linearized microcin J25 retains the structured core of the native macrocyclic peptide and displays antimicrobial activity doc
... be maintained in the thermolysin-linearized
form. In the cyclic MccJ25, the stability is likely to be
reinforced by the constraining strength of the circular
backbone.
Interestingly, the stable ... MccJ25-L
1)21
was ascertained by MALDI-TOF MS on
an Applied Biosystem Applera (USA) Voyager De-Pro
system used in a positive linear mode, with sinapinic acid as
a matrix....
Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx
... biocompatibility of nanomaterials.
Abbreviations
DIV 8, day (in vitro) 8; FAS, fatty acid synthase; FFA, free fatty acid; HIF- 1a, hypoxia-inducible factor- 1a; HIFs, hypoxia-inducible transcription
factors; ... Shvedova AA, Castranova V, Kisin ER, Schwegler-
Berry D, Murray AR, Gandelsman VZ, Maynard A &
Baron P (2003) Exposure to carbon nanotube material:
assessment of nanotub...
Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx
... Indeed, the change
in the array of the hydrogen bonds at thymine of
SRE
GGfos
is probably a sign of perturbation in the
hydration scheme along the minor groove of the (A ⁄ T)
domain. The G–C base pair ... temperature-induced struc-
tural changes in the Raman spectra during premelting
are mainly characterized, in SVD analysis, by variation
in the V
2
cont...
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