Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
... liver. Furthermore, we investigated the
speci®city of the human CBG with r espect to the glycone
and aglycone moieties, and in particular characterized the
ef®ciency of the enzyme in hydrolysing a ... reduced by 98% in the
presence of 4NP-b-
D
-mannopyranoside (10 m
M
)].
Hydrolysis of xenobiotic glycosides by reCBG
The a bility of CBG to hydrolyse a variety of...
... allowing the interaction of
Antarctic b-tubulin isotypes with CCT in the adverse
energetic conditions of the Antarctic habitat. The role of
these amino acid substitutions in the folding process of
E. ... analysis
of the interaction between CCT and b-tubulin by cryoelec-
tron microscopy allowed the identification of three regions
from the N-terminal, and five from...
... expression profiling of human
erythrocyte could be an important key for under-
standing the machinery of anucleate protein synthesis
and its meaning in the pathophysiology of diseases.
Acknowledgements ... mechanism of globin m-RNA
stability in human RBCs.
Further experiments are needed to understand
the mechanisms and the biological meaning of these
findings. Bu...
... of
cystatin F is hypothetical. Although most cystatins inhi-
biting cathepsin L also inhibit cathepsin S, an inhibitory
activity of cystatin F against cathepsin S has not yet been
reported.
The ... 88–90 (cystatin C numbering).
Recombinant cystatin F expressed in Sf9 insect cells is
indeed glycosylated [5].
Compared to other cystatins, cystatin F is an unusually
specific inhibitor. Amo...
... a single
cell line expressing defined ratios of multiple protein units,
excessive screening is necessary, as each gene introduced
into the cell multiplies the complexity of the screening.
Others ... ratios. The result presented here
show that the fusion event typically does not alter the
expression level of the recombinant protein (Fig. 2B).
Furthermore, in the study...
... for the
C. roseus enzyme preparation. The reaction gives rise to the
end product cathenamine and involves 4,21-dehydrocory-
nantheine aldehyde as an intermediate. The enzymatic
hydrolysis of dolichantoside ... understanding the biosyn-
thetic mechanisms. The next steps are to search for the
participating genes and to clarify the regulation of product
synthesis, with the...
... Cu
2+
strongly
inhibited the activity of NaoA, and Mn
2+
slightly increased
the activity. This implies that there may be a Mn
2+
or Cu
2+
binding site in the enzyme; metal ions may also act in other
ways.
We ... (nitroalkane-oxidizing enzyme gene).
Expression of
naoA
in
E. coli
To study the function of the naoA gene, it is necessary to
obtain an adequate amount of...
... above, using these aromatic com-
pounds instead of 4-amino-3-hydroxybenzoic acid as sub-
strate.
Inhibition of the 4-amino-3-hydroxybenzoate-cleaving
activity by the substrate analogues (2-aminophenols,
catechols, ... 2,3-dioxygenase.
Dioxygenases catalyzing the fission of benzene rings are key
enzymes in the microbial metabolic pathways of aromatic
compounds. Most of these...
... The expression of glutathione reductase in the male reproductive
system of rats supports the enzymatic basis of glutathione function
in spermatogenesis
Tomoko Kaneko
1,2
, Yoshihito ... cysteine, and
glycine, via two ATP-con suming s teps involving c-g lut-
amylcysteine synthetase ( cGCS) and glutathione synthetase.
The other constitutes a recycli...
... and 5 independently showed the pro-
tein expression of Cx43 and Cx40 in the iliac arteries
of different groups.
Figure 1. Hematoxylin/eosin staining of the iliac arteries in the normal ... down-regulate the
elevated protein expression of Cx43, suggesting the rennin-angiotensin system (RAS) plays an
important role in the remodeling of gap junction...