Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc

Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

... liver. Furthermore, we investigated the speci®city of the human CBG with r espect to the glycone and aglycone moieties, and in particular characterized the ef®ciency of the enzyme in hydrolysing a ... reduced by 98% in the presence of 4NP-b- D -mannopyranoside (10 m M )]. Hydrolysis of xenobiotic glycosides by reCBG The a bility of CBG to hydrolyse a variety of...
Ngày tải lên : 08/03/2014, 16:20
  • 10
  • 775
  • 0
Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt

Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt

... allowing the interaction of Antarctic b-tubulin isotypes with CCT in the adverse energetic conditions of the Antarctic habitat. The role of these amino acid substitutions in the folding process of E. ... analysis of the interaction between CCT and b-tubulin by cryoelec- tron microscopy allowed the identification of three regions from the N-terminal, and five from...
Ngày tải lên : 23/03/2014, 21:20
  • 7
  • 500
  • 0
Báo cáo y học: "ene expression analysis of human red blood cells"

Báo cáo y học: "ene expression analysis of human red blood cells"

... expression profiling of human erythrocyte could be an important key for under- standing the machinery of anucleate protein synthesis and its meaning in the pathophysiology of diseases. Acknowledgements ... mechanism of globin m-RNA stability in human RBCs. Further experiments are needed to understand the mechanisms and the biological meaning of these findings. Bu...
Ngày tải lên : 03/11/2012, 10:58
  • 4
  • 398
  • 0
Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... of cystatin F is hypothetical. Although most cystatins inhi- biting cathepsin L also inhibit cathepsin S, an inhibitory activity of cystatin F against cathepsin S has not yet been reported. The ... 88–90 (cystatin C numbering). Recombinant cystatin F expressed in Sf9 insect cells is indeed glycosylated [5]. Compared to other cystatins, cystatin F is an unusually specific inhibitor. Amo...
Ngày tải lên : 21/02/2014, 01:21
  • 10
  • 536
  • 0
Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx

Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx

... a single cell line expressing defined ratios of multiple protein units, excessive screening is necessary, as each gene introduced into the cell multiplies the complexity of the screening. Others ... ratios. The result presented here show that the fusion event typically does not alter the expression level of the recombinant protein (Fig. 2B). Furthermore, in the study...
Ngày tải lên : 18/03/2014, 01:20
  • 6
  • 371
  • 0
Báo cáo Y học: Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids pot

Báo cáo Y học: Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids pot

... for the C. roseus enzyme preparation. The reaction gives rise to the end product cathenamine and involves 4,21-dehydrocory- nantheine aldehyde as an intermediate. The enzymatic hydrolysis of dolichantoside ... understanding the biosyn- thetic mechanisms. The next steps are to search for the participating genes and to clarify the regulation of product synthesis, with the...
Ngày tải lên : 24/03/2014, 03:21
  • 10
  • 650
  • 0
Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot

Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot

... Cu 2+ strongly inhibited the activity of NaoA, and Mn 2+ slightly increased the activity. This implies that there may be a Mn 2+ or Cu 2+ binding site in the enzyme; metal ions may also act in other ways. We ... (nitroalkane-oxidizing enzyme gene). Expression of naoA in E. coli To study the function of the naoA gene, it is necessary to obtain an adequate amount of...
Ngày tải lên : 31/03/2014, 08:20
  • 6
  • 255
  • 0
Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc

Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc

... above, using these aromatic com- pounds instead of 4-amino-3-hydroxybenzoic acid as sub- strate. Inhibition of the 4-amino-3-hydroxybenzoate-cleaving activity by the substrate analogues (2-aminophenols, catechols, ... 2,3-dioxygenase. Dioxygenases catalyzing the fission of benzene rings are key enzymes in the microbial metabolic pathways of aromatic compounds. Most of these...
Ngày tải lên : 21/02/2014, 01:21
  • 7
  • 490
  • 0
Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc

Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc

... The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis Tomoko Kaneko 1,2 , Yoshihito ... cysteine, and glycine, via two ATP-con suming s teps involving c-g lut- amylcysteine synthetase ( cGCS) and glutathione synthetase. The other constitutes a recycli...
Ngày tải lên : 17/03/2014, 17:20
  • 9
  • 494
  • 0
Báo cáo y học: "Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits"

Báo cáo y học: "Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits"

... and 5 independently showed the pro- tein expression of Cx43 and Cx40 in the iliac arteries of different groups. Figure 1. Hematoxylin/eosin staining of the iliac arteries in the normal ... down-regulate the elevated protein expression of Cx43, suggesting the rennin-angiotensin system (RAS) plays an important role in the remodeling of gap junction...
Ngày tải lên : 26/10/2012, 09:39
  • 8
  • 467
  • 0

Xem thêm

Từ khóa: