Báo cáo Y học: Growth inhibition of mammalian cells by eosinophil cationic protein pptx

... USA; 4 Department of Surgery, Keio University School of Medicine, Tokyo, Japan Eosinophil cationic protein (ECP), one of the major components of basic granules of eosinophils, is c ytotoxic to tracheal ... rowth-inhibitory eect was also attenuated by this modi®cation. These results suggest that growth inhibition by ECP is dependent on cell type and is cytostatic. Keyw...

Ngày tải lên: 17/03/2014, 17:20

... Germany; 4 Department of Medicine, Section Pathophysiology, Johannes Gutenberg University of Mainz, Germany; 5 Department of Biochemistry, Christian Albrechts University of Kiel, Germany Ciliary neurotrophic ... by protein A–Sepharose. Immune complexes were analyzed by SDS/PAGE [31] and visual- ized by fluorography using the fluorographic intensifier solution ÔAmplifyÕ (Amersham...

Ngày tải lên: 22/02/2014, 07:20

... a flow rate of 5 lLÆmin )1 at 25 °C. The carboxymethyl dextran matrix of the sensor chip was activated by a 30-lL injection of the mixture of 0.2 M 1-ethyl-3-[(3-dimethylamino)propyl]carbodiimide and ... a possibility of the protein protein association in vivo. Vectorial transfer of tRNA/aminoacyl-tRNA during mammalian translation elongation cycle Recently, the crystal struct...

Ngày tải lên: 21/02/2014, 15:20

... inhibit the enzymatic activity of PpTGase might be similar to that by which they inhibit the enzymatic activity of TGase 4. Hydrolysing activity of GTP was also found in the purified PpTGase protein ... significantly similar to those of mammalian TGases. Furthermore, GTP inhibited the enzymatic activity of PpTGase, which also displayed GTP-hydrolysing activ- ity. We conclude tha...

Ngày tải lên: 17/03/2014, 23:20

... REVIEW ARTICLE Dynamic mechanism of nick recognition by DNA ligase Alexei V. Cherepanov* and Simon de Vries Kluyver Department of Biotechnology, Delft University of Technology, Delft, the Netherlands DNA ... fragments, before they are removed by the 5¢)3¢-exonuclease activity of DNA polymerase [76] or by the action of specific RNases [77]. The B-to-A helix transition would not...

Ngày tải lên: 21/02/2014, 01:21

... Holliday junction binding and processing by the RuvA protein of Mycoplasma pneumoniae Stuart M. Ingleston 1 , Mark J. Dickman 2 , Jane A. Grasby 3 , David P. Hornby 2 , Gary J. Sharples 4 and ... Biology and Biotechnology, University of Sheffield, UK; 3 Krebs Institute, Centre for Chemical Biology, University of Sheffield, UK; 4 Department of Biological Sciences, University of Durha...

Ngày tải lên: 17/03/2014, 17:20

... cell morphology after induction of differentiation of HL-60 cells by dimethylsulfoxide and TPA. (A) HL-60 cells cultured in RPMI for 3 days (control for B), followed by cytospin analysis and staining (·1000). ... activity was dramatically diminished. Alternatively, hTR and hTERT may play a minimal catalytic activity in telomerase, while the assembly of other telomerase subunits m...

Ngày tải lên: 17/03/2014, 23:20

... multisubunit proteins. SIgA is the major immunoglobulin of external secretions and is composed of four different polypeptide chains produced by two distinct cells. Polymeric IgA (pIgA) is produced by B -cells ... above and analysed by fluorescence microscopy. Verification of SC production Transfected and fused CHO-K1 cells were analysed for production of total SC, by a dot b...

Ngày tải lên: 18/03/2014, 01:20

... activated by cystatin-sensitive peptidase activity to yield fully active tcu-PA. In a purified system, plactin dually modulates prothrombin activation, depending on the conditions of catalysis by Xa. ... methylcoumarin-7-amide (GGA-MCA) from Peptide Institute (Osaka, Japan); Spectrozyme TH (H-d-hexahydro- tyrosyl-Ala-Arg-p-nitroanilide), Spectrozyme Xa (methoxy- carbonyl-d-hexahydrotyros...

Ngày tải lên: 07/03/2014, 00:20

... Figure 2. Effect of pH on the growth of Staphylococcus aureus in AT1V (pH5.5, OPR is approximately 5) and AT2V (pH5.5, OPR is approximately 6). The pH value was ad- justed by adding 0.5 mol/L ... 1. The pH value and OPR of AT1V are 5.5 and approximately 5, and those of AT2V are 5.5 and approximately 6. The other test solutions were prepared by adjusting the pH of AT1V an...

Ngày tải lên: 26/10/2012, 09:39