Báo cáo khoa học: Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana pptx

... sequence and sequence alignment of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numbers on the right of the figure indicate the positions of the nucleotide sequence. The ... to the N-terminal portion of the allergen. Based on this partial sequence, two specific primers were designed and used together with the anchor pri- mer AP2...

Ngày tải lên: 07/03/2014, 12:20

... from cleavage of the signal peptide and the propeptide, which happens in the maturation process for every conotoxin [8], the removal of the two additional resi- dues Leu and Val at the C-terminus is rather ... +3 hydrogen bonds for Asn11(CO)–Trp14(HN) and Ser12(CO)– Thr15(HN), and confirmed by the slow solvent exchange kinetics of the amide protons of Trp14 a...

Ngày tải lên: 18/02/2014, 17:20

... composed of two each of the a and b subunits. Analysis of the N-terminal transit peptide of CLGAT Analysis of the CLGAT cDNA predicted a chloroplast transit peptide upstream from the N-terminus of CLGAT. ... composed of two subunits with different N-terminal amino acid sequences. Analysis of the corresponding cDNA revealed that these two subunits have m...

Ngày tải lên: 20/02/2014, 03:20

... middle and C-terminal region of the HdEG66, respectively, from the sequence similarity to the termite cellulase. Then, a forward primer F1 was synthesized on the basis of the N-terminal sequence of the ... sides of the cDNAsindicateprimersusedforthePCR.LengthsofthecDNAsare shown in the parentheses. (B) Structure of the genomic fragment, Hdcel-1 DNA. Open and cl...

Ngày tải lên: 20/02/2014, 23:20

... the hemolymph of several crustaceans has been well known since the beginning of the 20th century [34]. An evaluation of the literature revealed that purification of lectin from the hemolymph of ... increase in specific activity. Analysis of the lectin on SDS/PAGE gave a single band at apparent molecular mass of 34 kDa. The binding affinity of the lectin in th...

Ngày tải lên: 21/02/2014, 00:20

... Biochem. 269) 1897 Identification of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides present in the purified enzyme preparation ... physiological electron acceptor of the enzyme. The enzyme was not oxidized by the heterodisulfide of coenzyme M and coenzyme B, or by the homodisulfides of...

Ngày tải lên: 21/02/2014, 03:20

... Numbers on the left refer to the sizes of molecular markers. A B C Fig. 1. Expression analysis of ZHE1 and ZHE2 genes. (A) The result of the northern blot analysis probed with ZHE1 and ZHE2 cDNAs. Total ... incubation, suggesting further digestion of the 43 kDa product. Furthermore, their 36.5 kDa bands became broader than that for the 50 min incubation. The sequ...

Ngày tải lên: 07/03/2014, 04:20

... content in their latex; and (2) the pres- ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the protein concentration per milligram of dried latex, ... Fractionation and purification of the enzymes stored in the latex of Carica papaya. J Chrom B 790, 229–238. 32 Laemmli UK (1970) Cleavage of structural proteins durin...

Ngày tải lên: 07/03/2014, 11:20

... further study. The concentration of the proteins was determined by the method of Lowry et al. [14] and the purity was examined by SDS/PAGE [15]. Assay of human PKIb activity The activity of purified ... cm )1 bands canbeassignedtoa-helix in the human PKIb; the 1666 and 1247 cm )1 bands are the characteristic frequencies of the random coil structure. Meanwhile,...

Ngày tải lên: 07/03/2014, 15:20

... mL of the above mentioned culture medium: a total of 400 dishes were prepared and used in the further steps of the experiments. Fungal material Fod pathotype 2 was used in the experiments, as the ... from the tissues of carnation (Dianthus caryophyllus L). The enzyme, with a molecular mass of 43–45 kDa and a pI of 4.15, specifically methylates the hydroxy substi...

Ngày tải lên: 08/03/2014, 08:20