Báo cáo khoa học: The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed docx

... whether the aspartic proteinase genes are also expressed in the guard cells of leaves; if so, this could explain the greater expression of the AtPaspA1 gene observed in the light. The aspartic proteinases ... glycosylation; proteinase. We have been studying the aspartic proteinase genes in Arabidopsis with the goal of understanding their function in...

Ngày tải lên: 17/03/2014, 11:20

... considered to display differences in the fold of the main chains. None of these includes any of the binding sites, but some are located in the vicinity. Three of the regions with different conformation involve ... similar arrangement, and the side chain of Ser97 in VPRK is too short. One side of the loop is involved in the bind- ing of the P2–P4 residues o...

Ngày tải lên: 19/02/2014, 07:20

... To overcome the fact that the prosa z-score is dependant of the length of the amino acid sequence, the z-score was normalized using the natural logarithm of the sequence length [39]. The resulting ... helix and the FG loop correspond to that region of the PAS fold that is part of the region which tethers the PAS Table 4. Backbone root mean square deviation v...

Ngày tải lên: 19/02/2014, 12:20

... changed either in the presence or absence of the metal cofactor. By contrast, the sub- strate binding affinity of the RNase H domain proba- bly increases in the presence of the metal cofactor compared ... alter the interaction between the sub- strate and metal ion, such that the scissile phosphate group of the substrate and the Mg 2+ ion are arranged ideally. T...

Ngày tải lên: 06/03/2014, 22:21

... phenylalanine 108 supplied from the other subunit exists in the proximity of the x-end [24]. These results led to the hypothesis that the prenyl- chain of the product elongates in the space enclosed by a-helices ... in the center of the cavity and likely to act as the bottom of it in these structures; however, the structural information is inde- cisive with respe...

Ngày tải lên: 07/03/2014, 06:20

... deter- mining the kinetic features of PKH. However, the data for the catalytic efficiency of ginkgotoxin and DPN are of the same order of magnitude and reflect their antivita- min character, as they are ... the antivitamin character of both compounds and explains a depletion of cofactor formation in the organism in the presence of the antivitamins. The kineti...

Ngày tải lên: 07/03/2014, 10:20

... positions of the corresponding side chain, Arg220, the NADPH cofactor and the substrate analog hexose in the MUR1 structure (Fig. 11). Some of the distances between these groups are rather long ... Stereoview of the RMD monomer. The cofactor-binding domain and the substrate- binding domain are shown in aqua and light sand, respectively; the APPR portion of the...

Ngày tải lên: 16/03/2014, 01:20

... the relationship between the efficiency of protein N-myristoylation and the identity of the amino acids at positions 3, 6 and 7 of the con- sensus motif in plant target proteins with the use of ... proteins of the expected size. The positions of pro- tein size standards are shown on the left of the gel. Table 1. Observed and calculated mass values for the...

Ngày tải lên: 29/03/2014, 21:20

... the expression of the rat, mouse and human homologues of ERO1-Like protein a but not of the isoform ERO1-Lb are stimulated by hypoxia in rats vivo and in rat, mouse and human cell cultures. The temporal ... speculate from these findings that the expression of ERO1-La is probably regulated via the HIF-pathway and thus belongs to the family of classic oxygen regulated...

Ngày tải lên: 31/03/2014, 01:20

... to determine to what extent they follow the rules accounting foridentitiesinprokaryotesandeukaryotes[1,4,5].Inspite of the universality of the genetic code, there are often barriers to aminoacylation ... accepted 1.1 nmol of serine per A 260 unit of tRNA. The following archaeal tRNAs were prepared by in vitro transcription [20] of their synthetic genes, constructed accord...

Ngày tải lên: 19/02/2014, 12:20