Báo cáo khoa học: Action of palytoxin on apical H+/K+-ATPase in rat colon pdf

... yet been demonstrated. Thus, in the current investigation, we describe the action of palytoxin on the H + /K + -ATPase from the rat colon and demonstrate that the interaction of this toxin with the ... latter findings, as the palytoxin- induced conductance was insensitive to 1 m M ouabain while being inhibited at low concentrations of orthovanadate. Similar findings showing a...

Ngày tải lên: 17/03/2014, 11:20

... volumes of cold NaCl/P i containing 10 m ML -arginine. The radioacti- vity corresponding to the incorporated L -[2,3,4- 3 H]arginine was directly measured by liquid scintillation counting of the ... Moreover in endothelial cells [36] extracellular L -arginine concentration is the most determinant of L -arginine availability for cNOS, despite the fact that intracellular arginine concen...

Ngày tải lên: 08/03/2014, 02:20

... Patient of type FORMULA is a subformula embedded within a FORMULA in the Actor relation with respect to the head contain’] contain( , ) CONTAINMENT(container , containee ) [’the Containment relation ... aforementioned publications. 4.3 Domain interpretation The linguistic meaning representations obtained from the parser are interpreted with respect to the domain. We are constructing a dom...

Ngày tải lên: 17/03/2014, 06:20

... Solid line: the case of kinase sequestration, dotted line: no kinase sequestration. Grey lines indicate the stimu- lus (i.e. kinase concentration), scaled by 0.1 in (A). Effects of sequestration ... concentration and total kinase concentration, respectively, and K M is the Michaelis–Menten constant of the kinase. The con- centration of the complex [TK] approaches the total concentra...

Ngày tải lên: 19/02/2014, 07:20

... the visualization of the protein composition of the fractions. Ryanodine-binding Ryanodine-binding assays were carried out using [ 3 H]ry- anodine in a medium having the composition of 1 m NaCl, S. ... aprotinin, 1 lm leupeptin, 1 lm pepstatin A and 1 mm benzamidine. Protein concentration of HSR vesicles was determined according to the method of Lowry et al. [21], using bovine ser...

Ngày tải lên: 19/02/2014, 16:20

... complete inhibition of TNFa- primed/fMLP-stimulated MPO release under conditions in which O À 2 generation was only marginally suppressed (Fig 4). Butan-1-ol alone, up to a concentration of 3%, ... [ 3 H]PtdBut accumulation over untreated controls. Inserts show the concentration effects of brefeldin A or wortmannin on O À 2 generation and PLD activity. Data represent a single exper...

Ngày tải lên: 07/03/2014, 15:20

... mutation consists of a single G fi A transi- tion in codon 237, resulting in a missense mutation of methionine to isoleucine [32,33]. Interestingly, a study conducted by Van Meir et al. on different ... that TQ preferen- tially binds to the active site at low concentrations, resulting in the observed inhibition, whereas at higher concentrations the binding to the additional site be...

Ngày tải lên: 15/03/2014, 11:20

... stoichiome- tries of participation of water, cations and anions in specific and non-specific binding of lac repressor to DNA. Possible thermodynamic origins of the ‘‘gluta- mate effect’’ on protein–DNA interactions. ... concen- trations or low temperatures, the gain in entropy on release of ions is reduced and substrate binding is therefore weaker [33]. This makes binding of...

Ngày tải lên: 16/03/2014, 06:20

... detectable binding to CRP at the concentration and during the period of observation used in the experiment. Interaction analyses using increasing concentrations of the native and of the low-pH ... antigen– antibody interaction in the presence of increasing concentrations of NaCl. In both experiments, binding intensities are represented in RU, and each data point shows the me...

Ngày tải lên: 16/02/2014, 15:20

... 57, 858–865. 36 Nakagawa Y, Minakuchi C, Takahashi K & Ueno T (2002) Inhibition of [3H] ponasterone A binding by ecdysone agonists in the intact Kc cell line. Insect Bio- chem Mol Biol 32, ... acid alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors. Fig. S2. Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ⁄ R...

Ngày tải lên: 18/02/2014, 08:20