Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf

... Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme Eric Record 1 , Peter J. Punt 2 , ... pLac1-A and pLac1- B contained the laccase cDNA corresponding to the laccase gene, lac1 from P. cinnabarinus (GenBankaccessionnoAF 170093) (Fig. 1). In pLac1- B, the 21 amino acids of...

Ngày tải lên: 17/03/2014, 11:20

... further used in POS tagging in (Ng and Low, 2004). One main disadvantage of this model is the difficulty in incorporating the whole word information. The second kind of solution is the “word-based” method, ... Seg C and SegTag L are the predictions of the three coarse- grained solvers. For the three words at the begin- ning and the two words at the end, t...

Ngày tải lên: 17/03/2014, 00:20

... moderate staining in the interstitium (purple arrows). (C–E) Staining of sPLA 2 -V in three active RA tissues. In all cases, intense staining of the granulation tissue in the sublining interstitium ... staining of sPLA 2 -IIE was observed in two inactive RA sections (Fig. 4A), it was intense in VSMC in three distinct active RA sections (Fig. 4Ba– c). In contrast, s...

Ngày tải lên: 19/02/2014, 16:20

... activation of protein kinase C and the mobilization of intracellular Ca 2+ , leading to the formation of a complex containing Src-family kinases, the focal adhesion kinase, Pyk2, and PI3K [33]. Similar ... promote the expression of the tumour necrosis factor (TNF) receptor- associated death domain and of the TNF-related apoptosis-inducing ligand, leading to the...

Ngày tải lên: 16/03/2014, 05:20

... phosphoinositide binding [31]. The five-amino-acid insertion in the loop of Noxo1 might alter the configuration of the phospho- inositide-binding region, affecting the affinity for Expression and function ... in the testis and fetal brain. The Noxo1c protein contains an additional five amino acids in the N-terminal PX domain, a phosphoinosi- tide-binding module; t...

Ngày tải lên: 19/02/2014, 06:20

... into t he expression vector following the manufacturer’s instruction (Gateway, Invi- trogen). The correct insertion of the Rv1399c ORF was confirmed by DNA sequencing. In our hands, protein expression ... [23]. Results and Discussion Cloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of  31.7 kDa and...

Ngày tải lên: 07/03/2014, 16:20

... included the mitochondrial single- stranded binding protein (mtSSB), and the catalytic (a )and accessory (b) subunits of the DNA polymerase c (P ol c) [24,25]. Interestingly, the expression of the genes ... identification of several transcription factors and coactivators that regulate the expression of genes playing key r oles in the biogenesis of the OXPHO...

Ngày tải lên: 07/03/2014, 16:20

... calculated using the forcefield of discover3 and used to anchor the pyranose ring of the sugars into the binding sites of the lectin. The position of mannose observed in the Man–banana lectin complex ... the cytoplasm and the nucleoplasm, including the nucleo- lus (Fig. 1). Purification and characterization of recombinant Orysata expressed in Pichia p...

Ngày tải lên: 14/03/2014, 23:20

... regulation of gene expression and modulation of kinetic properties of enzymes emphasizes the need for tight regulation of the level of nucleotides in the cell. Indeed, expression of genes responsible ... the only reaction resulting in the amination of the pyrimidine ring into a cytosine derivative (Fig. 1). It is therefore of interest to examine to what...

Ngày tải lên: 16/03/2014, 16:20

... 2010) doi:10.1111/j.1742-4658.2010.07770.x To understand the potential paracrine roles of interleukin-1b (IL-1b), tumour necrosis factor-a (TNF-a) and interleukin-10 (IL-10), the expres- sion and secretion of these factors by rat ... transplantation in rat models of myocardial infarction has anti -in ammatory effects, decreasing protein production and gene expression for...

Ngày tải lên: 18/02/2014, 04:20