Báo cáo khoa học: Functional expression of Pseudomonas aeruginosa GDP-4-keto6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose docx

... Functional expression of Pseudomonas aeruginosa GDP-4-keto- 6-deoxy- D -mannose reductase which synthesizes GDP-rhamnose Minna MaÈki 1 , Nina JaÈ rvinen 1 , ... GDP-4-keto-6-deoxy- D -mannose reductase (RMD). Here, we describe the enzymatic c har- acterization of P. aeruginosa RMD expressed in Sacchar- omyces cerevisiae. Previous success in functional expression o...

Ngày tải lên: 17/03/2014, 11:20

... characterization of quinoline- degrading bacteria. Syst. Appl. Microbiol. 10, 185–190. Ó FEBS 2003 Expression of quinoline 2-oxidoreductase genes (Eur. J. Biochem. 270) 1577 Functional expression of the ... availability of a system for the functional expression of genes coding for molybdenum hydroxylases is a prerequisite for the construction of enzyme variants by mutage...

Ngày tải lên: 17/03/2014, 10:20

... the control of the GAL10 pro- moter. The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene ... Identifica- tion of ligands for olfactory receptors by functional expression of a receptor library. Cell 95, 917–926. 20 Hatt H, Lang K & Gisselmann G (2001) Functional exp...

Ngày tải lên: 07/03/2014, 16:20

... 1.647 nm (confidence interval of )0.5963 to 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28), respectively. These ... versions of GEV format switches were made by replacing helix 12 of EcR with VP16 activation domain. This version of GEV supported constitutive expression of reporter...

Ngày tải lên: 16/03/2014, 12:20

... 1469 activity which was apparently produced by the E. coli expression strain. Expression pattern of mce-1::gfp fusion constructs in C. elegans To determine the expression pattern of the MCE-1, ... were grown to an optical density of 0.6–0.8 at 600 nm. Then, expression of the epimerase was induced by the addition of isopropyl thio-b-d-galactoside to a final concentration of...

Ngày tải lên: 23/03/2014, 13:20

... temperatures leads to differential expression of alternative forms of the LDH-A gene in white skeletal muscle of weatherfish, Misgurnus fossilis. Two isoforms of LDH-A mRNA were isolated and characterized: ... quantitative strategy to offset the lack or excess of kinetic energy (as a measure of temperature) and its effect on enzyme activity. Alternatively, we know of a number...

Ngày tải lên: 19/02/2014, 02:20

... expression of Citrus FLS The unequivocal assignment of genes requires the functional characterization of the corresponding polypeptides, which has been occasionally neglected in the process of ... molecular evolution of diversity concerning the enzymes of flavonoid biosynthesis. We report the expression of highly active Citrus unshiu FLS in Escherichia coli and the purific...

Ngày tải lên: 21/02/2014, 03:20

... presence of provided structural CHH implies the level of the complexity of physiologi- cal regulation in crustaceans. Much less is known of the functions of non- ES CHH than of those of its counterpart ... on the surface of the PO and many nerve fiber tracts and varicosities in trunks of the PO. Cloning and sequencing of PO-CHH cDNA The first PCR of PO cDNA with a combinat...

Ngày tải lên: 16/03/2014, 06:20

... activity of the P r form of CphBcy was clearly stronger than the activity of the P fr form, which reached, maximally, 20% of that of the P r form. Tak- ing into account the maximal conversion of P r into ... indicat- ive of the presence of a peptide with a bound chromo- phore (Fig. 3). None of these peaks matched the retention time of a free chromophore control sample. S...

Ngày tải lên: 30/03/2014, 08:20

... (1998) Clon- ing and sequencing of the nrdF gene of Corynebacterium ammoniagenes ATCC 6872 encoding the functional metallo-cofactor of the manganese-ribonucleotide reductase (Mn-RRase). Biotechnol ... we report data on homologous expression of the nrdF gene of C. ammoniagenes strain ATCC 6872. This is the first report of the successful purification of high amounts of the n...

Ngày tải lên: 15/02/2014, 01:20