Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

... 2002 Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile Eva Czerwiec 1 , Gail S. Begley 1 , Mila Bronstein 2 , Johan Stenflo 1,3 , ... the Conus carboxylase and vertebrate vitamin K-dependent c-carboxylases argue for conservation of a vitamin K-dependent carb- oxylase across a...

Ngày tải lên: 17/03/2014, 10:20

... the addition of poly(I)Æpoly(C) only managed to double A B Fig. 1. SDS ⁄ PAGE (A) and western blot analysis (B) of the affinity purified C-terminally and N-terminally His-tagged recombinant 2-5A synthetase ... nuclease and ⁄ or poly(I)Æpoly(C) on the 2-5A synthesizing activity of the recombinant porcine 2-5A synthetase (A) and N-terminally His-tagged recombinant 2-5A syn- th...

Ngày tải lên: 23/03/2014, 09:20

... refolding and purification they were characterized by both structural and enzyme kinetic studies. Mutation of Tyr183 t o phenylalanine produced an enzym e with a lower k cat and a slightly higher K m than ... cause of these differences is not known. K m and k cat measurements of hevamine and mutants Comparison of the steady-state kinetic parameters of hevamine and the T...

Ngày tải lên: 22/02/2014, 04:20

... connecting strand b5 and helix a3, while His290 and Asp260 e merge from the b8-a8andb7-a7 loops, respectively. Another important feature of the catalytic machinery is the so-called oxyanion hole necessary ... (50%) and have biochemically defined Rv1399c a s a non lipolytic hydrolase. This enzym e efficiently hydrolyzes short-chain synthetic substrates such as triacylglycerols and vi...

Ngày tải lên: 07/03/2014, 16:20

... S.G. (1994) Cloning and sequencing of thiol-speci®c antioxidant from m ammalian brain: alkyl reductases and thiol- speci®c antioxidant de®ne a large family o f antioxidant enzymes. Proc. N atl ... by HPLC and some of them were totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ( Fig. 2). Computer database searches based on the amino-acid sequ...

Ngày tải lên: 08/03/2014, 16:20

... for pen-3a transcripts and 18 S rRNA probes were quantified by STORM and analysed separately. Data analysis revealed an important individual variation in both pen-3a transcripts and 18 S rRNA ... constituted only 5% of the haemocytes analysed (Fig. 6A and B1). At 6, 12 and 48 h post-stimulation, this percentage increased, respect- ively, to 19, 23 and 34% of haemocytes displa...

Ngày tải lên: 18/03/2014, 01:20

... transient contraction by activating ryanodine receptors of the sarcoplasmic Fig. 1. Isolation of tigrin from the Duvernoy’s glands of R. tigrinus tigrinus. (A) The extract from Duvernoy’s glands ... nonreducing (lane 1–4) and reducing (lane 5–8) conditions. The positions of molecular mass markers are shown on the left. Lanes 1 and 5 show ablomin (26 and 29.7 kDa), lanes 2 a...

Ngày tải lên: 18/03/2014, 01:20

... Mg 2+ may contribute to the weak binding of Mg 2+ and enzymatic activity because proteins and enzymes generally bind Mg 2+ weakly [36]. Nevertheless, understanding the differences in binding and enzymatic ... appli- cation of this type of enzymes. Further studies aiming to elucidate the physiological and structural character- istics of PepD and related M20 family enzymes an...

Ngày tải lên: 23/03/2014, 06:20

... Zhang 2 and Tadashi Yoshida 2 1 Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan; 2 Department of Biochemistry, Yamagata University School of Medicine, Japan An ... groups of chlorophyll and phycobilin. Chlorophyll, which is contained in all plants including cyanobacteria and protoflorideophyceae, is synthesized from protoporphyrin IX,...

Ngày tải lên: 23/03/2014, 20:22

... Identification and characterization of thioredoxin and thioredoxin reductase from Aeropyrum pernix K1 Sung-Jong Jeon and Kazuhiko Ishikawa National Institute of Advanced Industrial Science and Technology ... selenite, selenoglutathione, vitamin K and alloxan [9,19]. In addition to TR having been isolated and characterized from a wide variety of bacterial and eukaryo...

Ngày tải lên: 23/03/2014, 21:20