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Báo cáo Y học: Guanosine diphosphate-4-keto-6-deoxy-D-mannose reductase in the pathway for the synthesis of GDP-6-deoxy-D-talose in Actinobacillus actinomycetemcomitans potx

Báo cáo Y học: Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin a-sarcin potx

Báo cáo Y học: Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin a-sarcin potx

... modify the conformation of the protein but abolish the ribosome-inactivating activity of a-sarcin. In addition, the loss of the positive charge at that position produces dramatic changeson the interaction ... Glu58 as the general base during the first step of the reaction. The hydrolysis of the cyclicderivative is performed by the same groups, but their rolesare reversed [23]. In a-sarcin the same ... J.G.(1993) Kinetic study of the cytotoxic effect of a-sarcin, a ribosomeinactivating protein from A. giganteus, on tumor cell lines: proteinbiosynthesis inhibition and cell binding. Mol. Cell....
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Báo cáo Y học: Guanosine diphosphate-4-keto-6-deoxy-D-mannose reductase in the pathway for the synthesis of GDP-6-deoxy-D-talose in Actinobacillus actinomycetemcomitans potx

Báo cáo Y học: Guanosine diphosphate-4-keto-6-deoxy-D-mannose reductase in the pathway for the synthesis of GDP-6-deoxy-D-talose in Actinobacillus actinomycetemcomitans potx

... respectively) to the proteins enco-ded by ORFs in the clusters responsible for synthesizing the SPAs in other serotypes of A. actinomycetemcomitans. The biosynthetic pathway for GDP-6-deoxy-D-talose,Fig. ... GDP-4-keto-6-deoxy-D-mannose reductase and the stereoselectivity of the reduction deter-mines the direction of synthesis of these two 6-deoxyhex-oses. However, neither the gene encoding the biosynthesis of GDP-6-deoxy-D-talose ... Guanosine diphosphate-4-keto-6-deoxy-D-mannose reductase in the pathway for the synthesis of GDP-6-deoxy-D-talose in Actinobacillus actinomycetemcomitans Nao Suzuki1, Yoshio Nakano1,...
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Báo cáo y học: "Elevated plasma homocysteine is positively associated with age independent of C677T mutation of the methylenetetrahydrofolate reductase gene in selected Egyptian subjects"

... Frequency of the MTHFR genotypes and the result of the Chi square testing in the different studied groups. Showing the frequency and the results of the cross tabulation of the T allele and the different ... significant increase in tHcy in the TT genotype individuals in the different groups. This finding supports more the lack of association between the presence of the T allele and the high tHcy levels in ... methyl glutaryl -CoA synthase enzyme ( the rate limiting enzyme in cholesterol biosynthesis) and hence hypercholesterolaemia. Furthermore, they suggested that elevated homocysteine increases the...
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Báo cáo y học: "Teaching child and adolescent psychiatry to undergraduate medical students - A survey in German-speaking countries"

... organization of topics to be taught. Alimitation of the present study is the lack of informationon the number of CAP staff and how many studentsthey teach. In the review by Sawyer et al. on teaching ... [16], the inclusion of questions onCAP in final medical examinationsisessential.IntheEuropean survey [3], 86% of the departments had anexaminationinCAP.GradesinCAPweregivenin34% of these departments. ... resources for teaching by the ministries of science and education is affected by the quality of teaching, and hence there is a strong incentiveto provide the students with effective ways of learning.As...
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Báo cáo y học: "Emulsified Isoflurane Preconditioning Reduces Lung Injury Induced By Hepatic Ischemia/Reperfusion in Rats"

... Emulsified isoflurane preconditioning markedly attenuated hepatic I/R-induced lung injury in rats, which may be hopefully applied to the clinical treatment of organ injury caused by hepatic surgery, ... incubated for 30 min with intermittent stirring. The suspension was centrifuged at 15,000g for 10min at 4℃, and the protein concentration was determined by Coomassie blue dye-binding assay. ... injury such as gut and liver I/R injury22. This process is as-sociated with activation of inflammatory reaction, including the increased activity of NF-κB and the in- creased inflammatory mediators...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"

... stimulate the pro-duction of reactive oxygen metabolite in the antral mucosa in vivo [23]. Hp can induce the production of pro-inflammatory cytokines from gastric epithelial cells resulting in the ... mtDNA resulting in abnormality in ATP produc-tion. Therefore, the amount of ATP was further de-termined aiming to indirectly detect the cell viability and the mitochondrial activity and fu nction ... markedly changed in the blank control and the negative control accompanied by absence of any mutation in the mtDNA. These findings further confirmed that the increased R OS levels and the elevated...
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Báo cáo y học: "Maitake Mushroom Extracts Ameliorate Progressive Hypertension and Other Chronic Metabolic Perturbations in Aging Female Rats"

... hydro-lyzed by ACE to furylacryloylphenylalanine (FAP) and glycylglycine. Hydrolysis of FAPGG results in a decreased absorbency at 340 nm. Serum ACE activity was determined by comparing the ... minutes post injection. The area under the curve was used to estimate activity of the NO system with an increase in SBP connoting augmented system activity. Cytokine Assay: Various cytokines ... this study was initiated on day 338 in the life of the female SD, the SBP of all 40 SD ingesting the control diet containing 20% sucrose had been rec-orded previously starting on day 50 of life...
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Báo cáo Y học: A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter ppt

Báo cáo Y học: A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter ppt

... ([Ca2+]cyt) is transduced by twomain pathways, one involving CaM and CaM-relatedproteins, and the other involving CDPKs. Both of thesepathways cross-talk at various points in the signalingcascade ... binding with DNA [27,53–55]. We have found that the binding of p40 is dependent on the dephosphorylationstatus of the protein and the binding is fully abolished once the protein is phosphorylated.Analysis ... kinase increasewas inhibited by the application of EGTA and W7, a CaMinhibitor. The purification of PsCCaMK using immuno-affinity chromatography resulted in coelution of the kinasewith another...
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Báo cáo Y học: Human bile salt-stimulated lipase has a high frequency of size variation due to a hypervariable region in exon 11 pot

Báo cáo Y học: Human bile salt-stimulated lipase has a high frequency of size variation due to a hypervariable region in exon 11 pot

... fat-soluble vitamin esters and cholesterolesters in the small intestine. In some species, includinghumans, the gene is also expressed in the lactatingmammary gland and the resulting protein is a constituent of ... con-®rming a different sequence of t he tail. The f unction of the tail has previously been discussed in the literature. Deletion of the tail by in vitro mutagenesis of the human enzyme wasshown ... signi®cantly decrease expression of the protein,presumably by affecting mRNA s tability [16]. F rom studieson the crystal structure of bovine BSSL it was concludedthat the terminal six hydrophobic...
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Báo cáo Y học: Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells pptx

Báo cáo Y học: Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells pptx

... brought in proximity with the ZAP70 tyrosine kinase, the mainphosphate-donor and thus activator, of these signalingproteins. These interactions are then of importance for the activation of both the ... associate with the Shb–PTB-Pro–GST fusion protein, containing both the PTB domain andtwo of the N-terminal proline-rich domains. This interac-tion was only slightly inhibited by phosphotyrosine. To ... stimulated by CD3 cross-linking for 2 min in 37 °C(+) before lysis in triton lysis buffer. (A) Cell extracts were incubated for 30 min on ice with the indicated immobilized fusion proteins in the presence...
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