... Emulsified isoflurane preconditioning markedly attenuated hepatic I/R-induced lung injury in rats, which may be hopefully applied to the clinical treatment of organ injury caused by hepatic surgery, ... incubated for 30 min with intermittent stirring. The suspension was centrifuged at 15,000g for 10min at 4℃, and the protein concentration was determined by Coomassie blue dye-binding assay. ... injury such as gut and liver I/R injury22. This process is as-sociated with activation of inflammatory reaction, including the increased activity of NF-κB and the in- creased inflammatory mediators...