Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf

... activation (Eur. J. Biochem. 269) 6151 Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions Hongmei Yang 1 , Charles H. Lin 2 , Gang Ma 1 , ... intrinsic transcriptional activity. Fig. 2. The transcriptional activity of IRF-3 depends on synergy between two sets of residues and is entirely de...

Ngày tải lên: 17/03/2014, 10:20

... progression of cancer; and the second question is whether the PR domain alone possesses anticancer activity. In this study, we quan- titatively analyzed the mRNA expression level of RIZ1 over ... changed every 2-3 days. The HuH7 cells were subcultured using 0.25% trypsin, 0.53 mM EDTA solution before reaching 100% confluence. Anticancer activity of PR domain by direct ad- mini...

Ngày tải lên: 25/10/2012, 11:15

... essential for erythroid differentiation. Genomic orientation and neighboring sequences may have functional implications for the interactions among the transcription factors. Many of the erythropoietic ... presents a scheme of the erythroid transcription- activation complex along promoters of erythropoietically active genes. GATA-2 All members of the GATA family of transcription fa...

Ngày tải lên: 08/03/2014, 22:20

... inhibitor-free control incubated under the same experimental conditions. Conversely, acid phosphatase activity was measured in the presence of NaF and of those of inhibitors that alter amidolytic activity. ... initially reported to be one residue upstream in this sequence, at the Tyr265±Gln266 bond [7]. Hydrolysis of this substrate by samples from the fractionation of seminal pla...

Ngày tải lên: 24/03/2014, 00:21

... concentration was determined by using a protein assay kit (Bio-Rad) with BSA as the standard. Enzymatic assay of phosphatase activity The phosphatase activity was measured by hydrolysis of 10 m M p-nitrophenyl ... measured at 410 nm (absorption coeffi- cient 16.6 m M )1 Æcm )1 ). Enzymatic assay of bromoperoxidase activity Assay of PhoN-Sf brominating activity. The brominating...

Ngày tải lên: 24/03/2014, 03:21

... Analysis: Rates in every 6 months periods of the study period were analyzed by com- parison of proportions with the chi-square test. Cor- relations between antibiotic resistance and consump- tion ... infection control committees many years before the initiation of the NARP. Even in these selected centres already apply- ing local antibiotic restriction policies the utilisation of m...

Ngày tải lên: 25/10/2012, 11:00

... For any of the non-self-complementary duplex formations, the thermody- namic parameters can be determined by two conventional van’t Hoff analysis methods. One is to plot the reciprocal of the ... Biochem- istry 40, 6971–6981. 56. Barnes, T.W. III & Turner, D.H. (2001) Long-range cooperativity in molecular recognition of RNA by oligodeoxynucleotides with multiple C5-(1-propynyl) py...

Ngày tải lên: 22/02/2014, 07:20

... Sauermann 1 1 Plant Physiology, University of Osnabrueck, Germany; 2 Planton GmbH, Kiel, Germany; 3 Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany Light/dark modulation of the higher ... specific cysteine residues mediated by the ferredoxin/thioredoxin system [1]. The activity of each of these enzymes is adjusted by fine-tuning, the rates of reduction and/...

Ngày tải lên: 08/03/2014, 09:20

... Department of Biochemistry and Molecular Biology, 2 CREST of Japan Science and Technology Corporation; 3 The Department of Nephrology and Endocrinology, Faculty of Medicine, The University of Tokyo, ... has a catalytic activity for the reduction of the 13,14-double bond of 15-keto-PGs in the presence of NADH or NADPH, and the oxidation of the 12-hydroxy group of LTB 4 in...

Ngày tải lên: 18/03/2014, 01:20

... perturbations of any system param- eter, not only concentrations of specific components, and that the direct effect of the perturbations does not need to be known. This is important as concentration ... representation of time derivatives, commonly used in systems theory, that avoids any approximations, thereby leading to significantly improved estimation results. Finally, we address the...

Ngày tải lên: 07/03/2014, 17:20