Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx

... subunits of toluene /o-xylene monooxygenase (Eur. J. Biochem. 269) 5699 Expression and purification of the recombinant subunits of toluene/ o -xylene monooxygenase and reconstitution of the active complex Valeria ... characterization of the recombinant subunits of the complex of toluene /o-xylene monooxygenase was car- ried by testing t...

Ngày tải lên: 17/03/2014, 10:20

... circumvent the rapid inactivation and renal clearance of GLP-1 is therefore an important step toward applying them therapeutically [5, 6]. Type 2 diabetes is characterized by insulin re- sistance and ... His-tag is often used for protein purification [11]. The affinity of the His-tag for metal ions allows the fusion product to be quickly separated from the bulk of ot...

Ngày tải lên: 25/10/2012, 11:10

... judged by SDS/ PAGE. The activity of the pure recombinant protein was 80% of that of t he wild-type protein in both the lysozyme and chitinase assays. Attempts to further purify the recombinant ... atom of the N-acetyl group of the )1 sugar residue, o rienting the carbonyl oxygen towards the C1 atom. Tyr183 is believed to assist Asp125 in this function by hydro...

Ngày tải lên: 22/02/2014, 04:20

... assembly of the V-ATPase complex in the former, while no assembly occurred in the latter. DISCUSSION Yeast V-ATPase is the best characterized member of the V-type ATPase family. Biochemical and ... addition, they found that the Vma3p was indispensable for the assembly of subunits A and B. Hirata et al. [19] investigated the functions of Vma11p and Vma16p in...

Ngày tải lên: 08/03/2014, 23:20

... level in the two systems is related to the efficiency of carboxylase expression in these systems. The specific carboxylase activity of the Conus carboxylase is very similar when expressed in either ... 2002 by the addition of proFIX18 and proPT18, indicating that the Conus carboxylase, like the bovine carboxylase, is activated by the human propeptides. To compare the...

Ngày tải lên: 17/03/2014, 10:20

... of granule content within the haemocyte cytoplasm. This quite original phenomenon appears to be followed by the lysis of the haemocytes and the release of cytoplasm content, as suggested by the ... of the mechanisms of penaeidin release from haemocytes, and determination of any potential involvement of penaeidin in the elimination of microorganisms via phagoc...

Ngày tải lên: 18/03/2014, 01:20

... degree of accuracy, but only if obtained on the same gel. The density of UCP3 protein (i.e. the intensity of the signal expressed per mg of proteins) was similar in the SS mitochondria of the two ... concentrations of recombinant protein are shown under the figures. The times of exposure werethesameforFig2AandB. The reproducibility of the Western blot qua...

Ngày tải lên: 24/03/2014, 04:21

... are expressed in the same tissues and cell types and whether these isoforms play thesamerole. To address the first of these questions, we have now studied the expression of aralar1 and citrin throughout mouse ... amounts of the protein and the densities of the immunoreactive bands. The analysis indicated that spleen, heart (particularly the ventricle), ovary,...

Ngày tải lên: 24/03/2014, 04:21

... determined the activities of citrate synthase (an enzyme of the Krebs cycle re¯ecting the number of mitochondria [10]), succi- nate dehydrogenase and cytochrome oxidase (compo- nents of the respiratory ... to glucose may not be due to the lack of the glucose-sensor system. However, the situation may be more complex because of differences in transcription and tr...

Ngày tải lên: 31/03/2014, 15:20

... practi- cally absent from the murine placenta, the mouse may prove useful for the study of physiological roles of PAPP-A outside this tissue. Development of the mouse as a model for the study of PAPP-A ... Importantly, these data support the development of the mouse as a model organism for the study of PAPP-A, which must take into account the differences between...

Ngày tải lên: 31/03/2014, 21:21