Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
... Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid Klaus Brandenburg 1 , ... respectively [2,3]. These observations suggested that other factors may be involved in the activation of m-calpain because, clearly, the millimolar levels...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y pot
... as to why C34 1V exhibits an increased DG à compared with the wild-type enzyme. As the properties of Val, such as size and hydrophobicity, are similar to those of Cys and the affinity of C34 1V for ... Single bands on SDS/PAGE analysis verified the purity of all mutants. The protein yields of C341G, C341S and C341D were similar to that of the wild-type, where...
Ngày tải lên: 08/03/2014, 23:20
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... together the substrate-binding and catalytic functions in proximity for effective enzyme activity. Two LPL mutants lacking the lipase activity – one a catalytic site mutant (S132T) and the other ... cotransfection of S132T and WWW mutants. The lipase and esterase activities after transfection of vectors were assayed (B). Gray bar indicates the recovery in LPL activity afte...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx
... neurotoxic. Alternatively, APP can be hydrolyzed by the b secretase activity at the N-terminus of Ab, which is successively released by the c secretase after cleavage within the membrane [6] either ... that the mechanism of membrane interaction and destabilization is different in the case of Ab, but it is fair to say that the similarity with the fusion domain...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Solution structure of the HIV gp120 C5 Domain pptx
... (1996) Anti-HIV activity of multibranched peptide constructs derived either from the cleavage sequence or from the transmembrane domain (gp41) of the human immunodeficiency virus type 1 envelope. Virology ... Importantly, the C5 domain employed in the present study does not possess any tryptophan residues. Therefore, we performed fluorescence titrations of the HIV gp41 e...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
... from the human H407A mutant, where the basal activity increased dramatically, and the mouse Q404H mutant, where the basal activity decreased by more than 50% (Fig. 4). The basal activity of H407Q ... receptors we may anticipate a more detailed dynamic picture of the transition from a silent to an activated nuclear receptor and the role of heterodimer formation...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
... does have some interaction with the N-terminal domain, the mechanism of regulating the catalytic activity may be similar to that of the dimer. In other word, the active site would be physically blocked ... Guo 1 , Yiwei Liu 1 , Yixin Sun 1 , Yu Gao 1,2 , Sheng Ye 1 , Guangtao Li 2 , Zihe Rao 1 , Boqin Qiang 2 and Jiangang Yuan 2 1 Laboratory of Structural Biology and...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc
... [44]. The presence of the charges in the C-terminal face of RIIa D/D may be essential to provide flexibility for the transition of the inactive holoenzyme (with bound catalytic subunit) to active form ... of the polar side chains of Gln23 and Gln24 in the top-bottom compared to the top- top structure. These observations, together with the exper- imental...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo Y học: Transcriptional regulation of erythropoiesis Fine tuning of combinatorial multi-domain elements ppt
... Israel; 2 Department of Hematology, The Tel Aviv Sourasky Medical Center, Tel Aviv and Tel Aviv University, Israel Haematopoiesis, the differentiation of haematopoietic stem cells and progenitors into various ... consequences, which together confirm the vital role played by specific domains in the corresponding transcription factors during in vivo erythroid and megakaryocyte develo...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo Y học: Domain organization, folding and stability of bacteriophage T4 fibritin, a segmented coiled-coil protein docx
... the unit-volume heat capacity of water (calÆdeg )1 ÆmL )1 ) (stored in Origin). The thermal coefficient of cubic expansion of tantalum is 0.00002. The values of the van’t Hoff enthalpy of the process ... helicity at this temperature and changes in the magnitude of the accompanying enthalpy. The ratio o f the van’t Hoff enthalpy to calorimetric enthalpy of 0.39...
Ngày tải lên: 24/03/2014, 03:21