Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx

... a lyase-isomeraseforthephotoactive3 1 -Cys-a84-phycoviolobilin chromophore of phycoerythrocyanin. Biochemistry 40, 12444– 12456. Ó FEBS 2002 Analyses of a-phycoerythrocyanin peptides (Eur. J. Biochem. 269) 5055 Purification, crystallization, ... 5055 Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides Georg...

Ngày tải lên: 17/03/2014, 10:20

... polyadenylation signal to be obtained by PCR and sequencing. The deduced amino- acid sequence of P. hilaris cellulase showed high homology to members of glycosyl hydrolase family 5 subfamily 2, ... a pen-type pH meter (model B-212; Horiba, Kyoto, Japan). Enzyme assay A carboxymethylcellulase (CMCase) assay was performed by measuring the amount of reducing sugars after incuba- tion of...

Ngày tải lên: 17/03/2014, 10:20

... 7.4, 5m M azide inhibited ADP hydrolysis by  70% whereas ATP hydrolysis was inhibited by only 10%. Inhibition of ADP and ATP hydrolysis by azide was greater when the pH of assay solutions was 6.4. Azide inhibition ... 0.2 and 0.64 depending on the pH and the presence of NP-40; (d) Fig. 3. Inhibition of ATP and ADP hydrolysis activities of purified chicken liver ecto-ATPDase...

Ngày tải lên: 22/02/2014, 04:20

... residues of the C. sepium b-amylase (Cys83, Cys96, Cys209 and Cys344) are homologous to those found in soybean b-amylase (Cys82, Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, ... method. Inhibition of the enzyme activity by glucose, maltose and cyclohexaamylose For the study of the enzyme inhibition by glucose, maltose and cyclohexaamylose b-amylase activ...

Ngày tải lên: 22/02/2014, 07:20

... Purification and biochemical characterization of some of the properties of recombinant human kynureninase Harold A. Walsh and Nigel P. Botting School of Chemistry, University of St Andrews, St Andrews, ... regulation of enzyme activity in vivo and consequent channeling of substrate 3-hydroxykynurenine down the tryptophan– kynurenine metabolic pathway. The purification of...

Ngày tải lên: 08/03/2014, 22:20

... Kalkkinen 1 1 Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland, 2 Norwegian College of Fishery Science, University of Tromsø, Norway, 3 Institute of Medical Biology, University ... R3 (PerSeptive Biosystems, Framingham MA, USA) tip, and a sample of the flow- through carrying the glycans was drop-dialysed and analysed by MALDI-TOF MS. RES...

Ngày tải lên: 08/03/2014, 23:20

... in hemagglutination, lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certain polysaccharides and glycoproteins [1,2]. Plant lectins isolated from a wide variety of plants ... molecular mass of 70 000 Da, while the MSL-2 and MSL-3 gave four bands with molecular masses of 42 000, 35 000, 25 000 and 19 000, and 35 000, 22 500, 17...

Ngày tải lên: 18/03/2014, 01:20

... mutagenesis [20], and later confirmed by analysis of the crystal structure [19]. Comparison of the active site of VanXY C with VanX- and VanY-type enzymes indicated that all of these enzymes contained ... parameters of D59A for hydrolysis of UDP-MurNAc-pentapeptide[Ala]. However, its activity was comparable with that of D59S and His 6 –VanXY C .The rates of hydrolysis of...

Ngày tải lên: 18/03/2014, 01:20

... [6]. LipA demonstrates hydrolytic activity toward emulsions of both medium and long chain triacylglycerols (Fig. 3). Areas of olive oil and tricaprylin hydrolysis are clearly seen and correspond with ... preferentially attacks medium length acyl chains (C 6 ,C 8 ). The enzyme demonstrates hydrolytic activity in emulsions of both medium and long chain triglycerides, as demonstrat...

Ngày tải lên: 23/03/2014, 21:20

... purification and catalytic proper- ties of a membrane-bound enzyme complex from C. hydro- genoformans composed of a hydrogenase and a CO dehydrogenases. The complex catalyzes the conversion of CO to ... polypeptides are hydrophobic as shown by the chloroform/methanol extr- action experiment. These polypeptides were in-gel trypsin or CNBr digested and the peptides obtained were an...

Ngày tải lên: 23/03/2014, 21:20