Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf

... 2003 RNA- binding of a PPR protein (Eur. J. Biochem. 270) 4081 RNA- binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA Takahiro Nakamura 1 , Karin ... better characterize the RNA- binding properties and the possible protein protein interactions of HCF152, we produced the protein and...

Ngày tải lên: 17/03/2014, 10:20

... detailed characterization of amylose synthesis both in vivo and in vitro. We show that GBSSI is capable of synthesizing a significant number of crystalline structures within starch. Quantifications of amount and type of ... rate of amylopectin synthesis. The increase in crystallinity wit- nessed during these first 12 h is in line with the high rates observed for amylopect...

Ngày tải lên: 22/02/2014, 07:20

... included SM22a, a known actin cross-linking protein, and BSA, which does not interact with or cross-link actin. In the absence of hhLIM, the majority of actin remained in the supernatant (S) and only a small ... dis- ruption of the second LIM domain of hhLIM abol- ished F-actin-binding activity, indicating the important role that LIM domain 2 plays in the control o...

Ngày tải lên: 16/03/2014, 06:20

... minireview, the expression of relaxin-3 in the brain, and particularly its functions, including the stress response and food intake, are described. Expression of relaxin-3 in the brain Relaxin-3 ... expressed in the brain and testis. In rodent brain, ana- tomical studies have revealed its predominant expression in neurons of the nucleus incertus of the dorsal p...

Ngày tải lên: 29/03/2014, 21:20

... Ca 2+ . Removing the proposed Ca 2+ -coordinating residues in positions 1 and 3 of the EF-hand motif significantly compromised the metal-binding capability of the engi- neered protein, indicating that the ... of investigating the Ca 2+ -binding sites in native Ca 2+ -binding proteins, we established a grafting approach to dissect their site- specific properties. This app...

Ngày tải lên: 07/03/2014, 00:20

... coordination of the Zn(II) ion bound at the C-terminal moiety of CeMT1 is con- tributed to by histidines, and the number of these involved in metal binding is analyzed below. Thus, the overall ... at all on the bind- ing abilities of the Crs5 MT of S. cerevisiae [12], we decided to continue our studies with the obtained clones. Recombinant synthesis and purification...

Ngày tải lên: 07/03/2014, 02:20

... proceed for 10 min, and the fluorescence was then measured at room tem- perature. In uence of interference The in uence of the main constituents of plasma on the determination of 2.00 · 10 )6 molÆL )1 GSH ... solution and showed high binding ability because of the two adjacent bis(b-CD)s. Because of the conformation of the linker of 1, the nitrogen atoms and a...

Ngày tải lên: 07/03/2014, 05:20

... its S 1 ¢ binding subsite. Substrate binding induces conformational changes involving the ArgA145- PheA146 fragment of the protein chain, acting as a flap, first opening and then closing the groove ... X-ray coordinates of amino acid residues in penicillin acylase [19] and AM1 docking calculations. The three N atoms of the side chain d-guanidino group of this residue are...

Ngày tải lên: 16/03/2014, 16:20

... H 2 O The regulatory protein (MMOB) plays several roles in the catalytic process including modulating the rate of electron transfer between MMOR and MMOH [4,5], altering the redox potential of the ... to glutathione S-transferase and prepared the recombinant truncate by the same method used for the full-length protein. Like the recombinant intact MMOB [11], the re...

Ngày tải lên: 17/03/2014, 09:20

... way of estimating LDA topic models. Current Bayesian PCFG inference algorithms re- quire time proportional to the cube of the length of the longest string in the training corpus, and since these ... generated by such a CFG. The relationship between the LDA model and the PCFG can be understood by studying the trees generated by the CFG. In these trees the left- br...

Ngày tải lên: 23/03/2014, 16:20