Báo cáo khoa học: Probing plasma clearance of the thrombin–antithrombin complex with a monoclonal antibody against the putative serpin–enzyme complex receptor-binding site docx
Báo cáo khoa học: Probing plasma clearance of the thrombin–antithrombin complex with a monoclonal antibody against the putative serpin–enzyme complex receptor-binding site docx
... T–AT complexes and the SEC receptor (Eur. J. Biochem. 270) 4069 Probing plasma clearance of the thrombin–antithrombin complex with a monoclonal antibody against the putative serpin–enzyme complex ... dissoci-ation constant. The rate of clearance of the ternaryT–AT–Fab complex in rats was only slightly slower than the rate of clearance of T–AT. Studies with culturedHepG2 cells indicated that ... stoichiometry of binding.In vivo clearance of the T–AT complex in ratsThrombin, native AT, and elastase-cleaved AT were labeled with 125I (Amersham Pharmacia AB, Uppsala, Sweden)using the chloramine...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... solution were applied to the wells. The absorbance of the samples was measured at450 nm using a microplate reader.Statistical analysisData are expressed as the mean ± SEM. Differencesamong groups ... cardiac fibrosis in a paracrine manner under ischaemic conditions.Taken together, these findings may improve understanding of the cellu-lar and molecular basis of the anti-inflammatory and paracrine ... sudden cardiac failure and death.Many researchers have investigated cell transplantationas an alternative treatment for heart disease. Bonemarrow-derived mesenchymal stem cells (MSCs) areeasily...
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Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... participates ingrowth regulation of human breast carcinoma cells.Oncogene 20, 2499–2513.9 Kanda N, Seno H, Konda Y, Marusawa H, Kanai M,Nakajima T, Kawashima T, Nanakin A, Sawabu T,Uenoyama Y ... when they are treated with IFN-c, we can tentatively conclude that it interacts with the activated forms of STAT3 and STAT1. The actions of STAT3 and STAT1 are highly entangled,they also have antagonistic ... STAT3 decoy ODN inhibits the transcriptional activity of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the cells with...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... forward)25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse)35¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ PsCBL (degenerate forward)45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ ... (degenerate reverse)55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward)65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR reverse)75¢-CGAGCTCACTGCCTCTCAAC-3¢ PsCBL (5¢UTR forward)85¢-ACTCGTAGC-ACAGAGACAGAG-3¢ ... Transgenicevaluation of activated mutant alleles of SOS2 reveals a critical requirement for its kinase activity and C-term-inal regulatory domain for salt tolerance in Arabidopsisthaliana....
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Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) pptx
... exception of the zebrafishliver and heart, the overall pattern of adult tissue distri-bution of zebrafish fapb6 and mammalian FABP6 ⁄ -Fabp6 gene transcripts, and the transcriptional initiation of these ... mamma-lian FABP6 ⁄ Fabp6 transcripts, which are also generallyfound in the ovary and adrenal gland. In fishes, the adrenal homolog is not as compact as the adrenal glandfound in mammals. In fishes, adrenal ... human FABP6 (55.3%) compared to other orthol-ogous mammalian FABPs and paralogous zebrafish Fabps. Phylogeneticanalysis showed that the zebrafish Fabp6 formed a distinct clade with the mammalian...
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Báo cáo khoa học: Phenylalanine-independent biosynthesis of 1,3,5,8-tetrahydroxyxanthone A retrobiosynthetic NMR study with root cultures of Swertia chirata docx
... each13C-NMR signal of a metabolitefrom the labelling experiment and of the same compound atnatural13C abundance [22]. The ratios of the signal integrals of the biolabelled compound and of ... and of the compound atnatural abundance were then calculated for each respectivecarbon atom. Absolute13C abundances for certain carbonatoms (i.e. for carbon atoms with at least one attachedhydrogen ... shikimatepathway of aromatic amino-acid biosynthesis (Fig. 1). The aromatic ring of phenylalanine reflects the labelling patterns of C2–C3 of phosphoenolpyruvate and of C1–C4 of erythrose 4-phosphate....
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Báo cáo khoa học: X-ray structure of peptidyl-prolyl cis–trans isomerase A from Mycobacterium tuberculosis potx
... The final absorbance was estima-ted from e ach curve; the absorbance at each time point wassubtracted from that value. A plot of the natural logarithm of these differences vs. time was linear ... show an rmsd of approxi-mately 1.6 A ˚from that of Mt PpiA, when the C a atoms arecompared using a cut-off of 3.5 A ˚ (with 88% of the Casmatching). This is significantly larger than the ... Kmvalues are not generallyavailable f or Ppis, kcathas b een estimated for the humanPpiA t o be 9000 s)1 with a different peptide [29]; takentogether with an available kcat/Kmestimated...
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Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion pot
... Additionally, there are two partiallyoccupied sulfate ions. One of them forms two hydro-gen bonds with the main chain nitrogen atoms of Asp74 (2.80 A ˚) and Ala67 (2.86 A ˚) and with a watermolecule ... group and within a van der Waals distance of a methine carbon atom (CHA) of the porphyrin ring. Inconsequence, Gln57 is capable of tuning the redoxpotential of the heme group [7,8].An insert of ... Crystallogr 56, 1577–1582.13 Akazaki H, Kawai F, Chida H, Matsumoto Y, Hiray-ama M, Hoshikawa K, Unzai S, Hakamata W, NishioT, Park SY et al. (2008) Cloning, expression and purifi-cation of...
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Báo cáo khoa học: "METONYMY: REASSESSMENT, SURVEY OF ACCEPTABILITY, AND ITS TREATMENT IN A MACHINE TRANSLATION SYSTEM S" ppt
... and Japanese. The dif- ferent languages show differences in accep- tability (for the details, Kamei and Wakao 1992). Based on both intuitive analyses and the result of the .survey, we have ... previous works (Lakoff and Johnson 1980, Fass 1991, Yamanashi 1987). We asked native speakers of the three languages to score the acceptability of each sentence. Direct translations were used ... (Fass 1991, Lakoff and 309 Johnson 1980, Yamanashi 1987) seem to be obtained from the intuition of the analysts. However, we think that the pat- terns which are based on the analysts' intuition...
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Báo cáo khoa học: Molecular identification of adrenal inner zone antigen as a heme-binding protein potx
... a monoclonal antibody raised against the fasciculata and reticularis zones of the rat adrenal, was previously found to be identical with a protein vari-ously named 25-Dx and membrane-associated ... molecule can be calculatedbased on the content of aromatic amino acids, and ourcalculation suggested that 10 lm GST-hIZA1 wouldhave an A 280 of 0.357 absorbance unit. On the otherhand, when we added ... that IZA1 is associated with the endoplasmicreticulum membrane. A BFig. 4. (A) The heme-binding capacities of the wild-type hIZA1 andmutants. The capacities were estimated by measuring the A 402⁄...
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