Báo cáo khoa học: Human proteoglycan testican-1 inhibits the lysosomal cysteine protease cathepsin L pdf

... data indicate that testican-1 is capable of modulating cathepsin L activity both in intracellular vesicles and in the extracellular milieu. Keywords: cathepsin L; proteoglycan; protease; testican; thyropin. Testican ... interactions. While cathepsin L is an intracellular protease localized within lysosomes under normal conditions, the protease is secreted when expressio...

Ngày tải lên: 17/03/2014, 10:20

... of these molecules has pro- gressed beyond the experimental stage into clinical trial, probably in part because these compounds are insufficiently drug-like. Little data is publicly available on ... displacement disregulates telomerase function [7,8]. Also, although the classic telomerase inhibition model does not appear to be fol- lowed by G-quadruplex-binding agents, cancer cells general...

Ngày tải lên: 06/03/2014, 09:22

... is generally observed [9,10] at the core of such peptides. Other than plant systems, a CT-extension found in a lysosomal cysteine protease (Lpcys2) of Leish- mania pifanoi, plays a role in the regulation ... et al. Role of C-terminal extension in a cysteine protease FEBS Journal 278 (2011) 3012–3024 ª 2011 The Authors Journal compilation ª 2011 FEBS 3013 molecular mass almost...

Ngày tải lên: 14/02/2014, 14:20

... alone most likely plays no role. Cathepsin B will accept inhibitors or substrates, whatever is available. Fig. 4. The extent of the occluding loop displacement (superim- posed). The papain-like ... stefin A fills the active site cleft along the whole length and displaces the occluding loop (the ‘lasso’ is shown in red). Table 1. Average distances between CA atoms of the stefins...

Ngày tải lên: 18/02/2014, 04:20

... Adjective Lexical error Adverb Lexical error Preposition Lexical error on normal and dependent preposition Article Lexical error Pronoun Lexical error Others Collocation error Figure 4. The ... these (the targeted word, the one preceding and one following/ the targeted word and the one preceding/ the targeted word and the one following/ the targeted word and the...

Ngày tải lên: 20/02/2014, 16:20

... can choose the best similarity metrics. Our main assumption is that all manual sum- maries are equally optimal and, while they are likely to be different, the best similarity metric is the one that ... produce larger Q values; and, depending on the scale properties of x, this cannot be solved just by scaling the final Q value. A probabilistic measure that solves this problem and sati...

Ngày tải lên: 17/03/2014, 05:20

... added as follows: hygromycin B (Sigma) at 50 lgÆmL )1 , phleomy- cin (Cayla 1 , Toulouse, France) at 1 0 lgÆmL )1 , and neo mycin (G418, Geneticin, Life T echnologies Inc.) at 25 lgÆmL )1 . Molecular ... with those of human recombinant cathepsin B and cathepsin L [21]. Therefore we also comparatively a nalyzed the carboxydipeptidase activit- ies of rCPB3 and r H84Y using the inter...

Ngày tải lên: 23/03/2014, 13:20

... 10 5 UÆmL )1 , 125 mL of Ham’s F12, 50 mL of fetal bovine serum, 500 lL of transferrin (Sigma), 5 mgÆmL )1 in 0.1% BSA in NaCl ⁄ P i , 500 lL of insulin (Sigma), 5mgÆmL )1 in 1 mm HCl, 500 lL of cholera ... pathogen-free holding rooms in the Princess Alexandra Hospital biological resources facility (Brisbane, Qld, Australia). The protocols of these experi- ments were approved by the ins...

Ngày tải lên: 29/03/2014, 00:20

... transfec- tion, cells were harvested and plated into 96-well plates at a concentration of 5 · 10 4 cells ⁄ well. The cells were then trea- ted with varying doses of chemicals diluted with 1% BSA ⁄ NaCl ⁄ P i (v ... 4-OHT, providing the space that fits the phenol group per- fectly. Replacement of Glu275 and Arg316 with structurally similar amino acids When Glu275 was replaced solely by gl...

Ngày tải lên: 18/02/2014, 16:20

... in the subcellular localization of Cdc45, together with an unchanged protein level during the cell cycle, were also obtained with T98G cells (data not shown). The data led us to conclude that human Cdc45 ... the turn-over of the essential replication factor Cdc45 have been available. There- fore, we evaluated the half-life of endogenous Cdc45 by radioactive metabolic labelling o...

Ngày tải lên: 19/02/2014, 00:20