Báo cáo khoa học: Synthesis and characterization of Pi4, a scorpion toxin from Pandinus imperator that acts on K+ channels doc

... scorpion toxin from Pandinus imperator that acts on K + channels Sarrah M’Barek 1 , Amor Mosbah 1 , Guillaume Sandoz 2 , Ziad Fajloun 1 , Timoteo Olamendi-Portugal 3 , Herve ´ Rochat 1 , Franc¸ois ... on Kv1.2 channels. Conclusions From a number of previous reports on different scorpion toxins that act on Kv-type channels, it appears that the toxin Fig...

Ngày tải lên: 17/03/2014, 10:20

... was mea- sured at pH 6.0 for 10 min at two Gly-Sar concentrations and at increasing Bip-Pro concentrations. The diffusional component of [ 14 C]Gly-Sar uptake, of 8% in Caco-2 cells and of 4% ... was inhibited not only by unlabeled Bip-Pro itself, but also by well known sub- strates of H + ⁄ peptide cotransporters, such as Gly-Sar, Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala...

Ngày tải lên: 07/03/2014, 05:20

... C)AATTTC-3¢ PsCBL (degenerate forward) 45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse) 55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward) 65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR ... T)GC(C ⁄ G ⁄ T)AAGGT-3¢ PsCIPK (degenerate forward) 25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse) 35¢-CTTAT(C ⁄ G)AACAAGGAA (A...

Ngày tải lên: 19/02/2014, 07:20

... cation exchange chromatography of the IB fraction. In the latter case, only a single peak was detected, eluted at the same concentration of ammonium acetate as for the S preparation, again containing ... preparation) and the pelleted fraction, after solubilization and renaturation (see Methods), called IB preparation. The S and IB preparations were fract- ionated in parallel by si...

Ngày tải lên: 30/03/2014, 15:20

... G.C., Katakura, K., Tomita, T., Zhang, X., Sun, D., Sato, M., Sasahara, M., Kayama, T., Ikeda-Saito, M. & Yoshida, T. (2000) Histidine 20, the crucial proximal axial heme ligand of bacterial ... the peak at 637 nm is much enhanced and new peaks Table 2. g-Values and g-anisotropy of alkaline forms of heme–heme oxygenase complexes. Data for Hmu O and rat HO-1 are taken from re...

Ngày tải lên: 23/03/2014, 20:22

... displayed hemagglutination and toxicity toward mice (data not shown), additional efforts to cleanly isolate the isoform were not successful and further characterization was abandoned. A chromato- focusing ... hemagglutination- inhibition assays Hemagglutination assays were carried out using normal human (A + ,B + and O + ), horse and rabbit erythrocytes in P. V. Castilho et al....

Ngày tải lên: 18/02/2014, 16:20

... TCA TCA TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAG GAG GGA TAT GGG GAA C. The PCR reaction was done as described above. The amplified PCR product was inserted ... intracellular tyro- sinases from N. crassa [26–28] and Agaricus [28] and Pycnoporus species [25] have an additional C-terminal domain that is proteolytically released from the c...

Ngày tải lên: 19/02/2014, 06:20

... follows: HSF 1a forward, 5¢-GAAGCAGCTTGTCCAGTACACCAA-3¢; HSF 1a reverse, 5¢-TTCCAAGAGCTGAACAAACCATTG-3¢; HSF1b forward, 5¢-GAAGCAGCTGGTCCAGTACAC CTC-3¢; HSF1b reverse, 5¢-GGCTGAATAAACCATGC CAGTAGC-3¢; ... (5¢-CCCAGTCACGACGTTGTAAAA CG-3¢)asasenseprimerandHSF1-specific primers (for HSF 1a, 5¢-GAAGCAGCTTGTCCAGTACACTAA-3¢;for HSF1b,5¢-GAAGCAGCTGGTCCAGTACACCTC-3¢)as antisense primers. The nested...

Ngày tải lên: 19/02/2014, 12:20

... dynamics reveals the nuclear degradation of ribosomal proteins. Curr Biol 17, 749–760. 21 Iadevaia V, Caldarola S, Tino E, Amaldi F & Loreni F (2008) All translation elongation factors and ... 5¢-noncoding region of poliovirus RNA: implications for internal translation initiation. Genes Dev 3, 1026–1034. 30 Zhu J, Hayakawa A, Kakegawa T & Kaspar RL (2001) Binding of the La au...

Ngày tải lên: 07/03/2014, 01:20

... template. One hundred and fifty thousand QC774 transformants were screened on 40 M63 minimal media agar plates containing 1 lm paraquat for each randomly point-mutagenized library. In addition, a ... the backbone amide and carbonyl of Cys140 form hydro- gen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity...

Ngày tải lên: 07/03/2014, 11:20