Báo cáo khoa học: Synthesis and characterization of Pi4, a scorpion toxin from Pandinus imperator that acts on K+ channels doc

... scorpion toxin from Pandinus imperator that acts on K+ channels Sarrah M’Barek1, Amor Mosbah1, Guillaume Sandoz2, Ziad Fajloun1, Timoteo Olamendi-Portugal3,Herve´Rochat1, Franc¸ois ... on Kv1.2 channels. Conclusions From a number of previous reports on different scorpion toxins that act on Kv-type channels, it appears that the toxin Fig. 7. Docking of sPi4 on rat voltage-gated ... Sabatier, J.M. (1996) Chemical synthesis and characterization of maurotoxin, a short scorpion toxin with four disulﬁde bridges that acts on K+ channels. Eur. J.Biochem. 242, 491–498.4. Kharrat,...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... was mea-sured at pH 6.0 for 10 min at two Gly-Sar concentrations and atincreasing Bip-Pro concentrations. The diffusional component of [14C]Gly-Sar uptake, of 8% in Caco-2 cells and of 4% ... was inhibited not only byunlabeled Bip-Pro itself, but also by well known sub-strates of H+⁄ peptide cotransporters, such as Gly-Sar,Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala,d-aminolevulinic ... 2007 FEBS Synthesis and characterization of a new and radiolabeledhigh-afﬁnity substrate for H+/peptide cotransportersIlka Knu¨tter1, Bianka Hartrodt2,Ge´za To´th3, Attila Keresztes3,...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... C)AATTTC-3¢ PsCBL (degenerate forward)45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse)55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward)65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR ... T)GC(C ⁄ G ⁄ T)AAGGT-3¢ PsCIPK (degenerate forward)25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse)35¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ PsCBL ... recognizes a consensus residue and based on that residue other amino acids that fall in that consensus position are marked. The most identical amino acids at each proteinare dark shaded and similar ones...

Báo cáo khoa học: Preparation and characterization of geodin A bc-crystallin-type protein from a sponge pot

... cation exchange chromatography of the IB fraction. Inthe latter case, only a single peak was detected, eluted atthe same concentration of ammonium acetate as for the Spreparation, again containing ... preparation) and the pelleted fraction, aftersolubilization and renaturation (see Methods), calledIB preparation. The S and IB preparations were fract-ionated in parallel by size exclusion chromatographyfollowed ... rather than that of a mammalian crystallin such as cS-crystallin, a qualitative, indirect indication of a closer structuralrelationship between geodin and protein S. Geodinalso displays a single...

Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx

... G.C., Katakura, K., Tomita, T., Zhang, X., Sun, D., Sato,M., Sasahara, M., Kayama, T., Ikeda-Saito, M. & Yoshida, T.(2000) Histidine 20, the crucial proximal axial heme ligand of bacterial ... the peak at 637 nm is much enhanced and new peaksTable 2. g-Values and g-anisotropy of alkaline forms of heme–heme oxygenase complexes. Data for Hmu O and rat HO-1 are taken from refs [24] and ... is the ﬁrstreport of the characterization of the isolated cyanobacterialHO-1 protein and its heme complexes, applying the opticalabsorption and electron paramagnetic resonance (EPR)spectroscopies.Experimental...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... displayed hemagglutination and toxicitytoward mice (data not shown), additional efforts tocleanly isolate the isoform were not successful and further characterization was abandoned. A chromato-focusing ... hemagglutination-inhibition assaysHemagglutination assays were carried out using normalhuman (A +,B+ and O+), horse and rabbit erythrocytes inP. V. Castilho et al. Characterization of four ... withinthe last 30 years. The greatest number of RIPs havebeen found in the Caryophyllaceae, Sambucaceae,Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and Poaceae [1]. Although many are potentially...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... TCA TCATCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The ampliﬁed PCR product wasinserted ... intracellular tyro-sinases from N. crassa [26–28] and Agaricus [28] and Pycnoporus species [25] have an additional C-terminaldomain that is proteolytically released from the cata-lytic domain. It has ... investigated fungaltyrosinases, from both a structural and a functionalpoint of view, are from Agaricus bisporus [10] and N. crassa [1]. Also, a few bacterial tyrosinases havebeen reported, of which...

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... follows: HSF 1a forward,5¢-GAAGCAGCTTGTCCAGTACACCAA-3¢; HSF 1a reverse, 5¢-TTCCAAGAGCTGAACAAACCATTG-3¢;HSF1b forward, 5¢-GAAGCAGCTGGTCCAGTACACCTC-3¢; HSF1b reverse, 5¢-GGCTGAATAAACCATGCCAGTAGC-3¢; ... (5¢-CCCAGTCACGACGTTGTAAAACG-3¢)asasenseprimerandHSF1-speciﬁc primers (forHSF 1a, 5¢-GAAGCAGCTTGTCCAGTACACTAA-3¢;forHSF1b,5¢-GAAGCAGCTGGTCCAGTACACCTC-3¢)asantisense primers. The nested PCR was performed ... act asmolecular chaperones that aid in the folding, assembly,degradation and translocation of intracellular proteins [1].The expression of HSP genes is regulated by heat shocktranscription...

Báo cáo khoa học: Synthesis and function of ribosomal proteins – fading models and new perspectives pptx

... dynamics reveals thenuclear degradation of ribosomal proteins. Curr Biol17, 749–760.21 Iadevaia V, Caldarola S, Tino E, Amaldi F & Loreni F(2008) All translation elongation factors and ... 5¢-noncoding region of poliovirus RNA: implications for internal translationinitiation. Genes Dev 3, 1026–1034.30 Zhu J, Hayakawa A, Kakegawa T & Kaspar RL (2001)Binding of the La autoantigen ... perspectivesSara Caldarola, Maria Chiara De Stefano, Francesco Amaldi and Fabrizio LoreniDepartment of Biology, University ‘Tor Vergata’, Roma, ItalyIntroductionRibosomal proteins (RPs) are fundamental...

Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx

... template. One hundred and ﬁfty thousand QC774 transformants were screened on 40 M63 minimal media agar plates containing 1 lmparaquat for each randomly point-mutagenized library.In addition, a ... thebackbone amide and carbonyl of Cys140 form hydro-gen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of theactive site cavity and the replacement ... CA). Allagarose gels used contained 1% agarose. Gel extractions of DNA from agarose gels were performed with the QIAEX IIgel puriﬁcation kit (Qiagen). Puriﬁcation of standard PCRproducts from...

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