Báo cáo khoa học: Key substrate recognition residues in the

Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1 ppt

... 5¢-GAAGTTAAAGATACAATGATTCAGCTC 5¢-GAGCTGAATCATTGTAACTTTAACTTC-3¢ 48 N302D 5¢-CATTGTTGAAGACATCAATGTTG-3¢ 5¢-CAACATTGATGTCTTCAACAATG-3¢ 43 N302F 5¢-CTTTACATTGTTGAATTCATCAATGTTGCAGC-3¢ 5¢-GCTGCAACATTGATGAATTCAACAATGTAAAG-3¢ ... 5¢-CAACATTCCTTGTCATCGAACCAAACTC-3¢ 58 R103M 5¢-GTTCGAGAACAATGAATGTTGTGTTC-3¢ 5¢-GAACACAACATTCATTGTTCTCGAAC-3¢ 55 R103E 5¢-GAACACAACATTCTCTGTTCTCGAACC-3¢ 5¢-GGTTCGAGAACAGA...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo khoa học: Post-translational modiﬁcations in the active site region of methyl-coenzyme M reductase from methanogenic and methanotrophic archaea potx

... Methanosarcina barkeri) or directly after the thioglycine (Methanosarcina barkeri), and an alanine instead of an aspartate two positions from the thioglycine (Methanopyrus kandleri). The cysteine ... [2]. Methanogenic archaea and methanotrophic archaea all belong to the kingdom of Euryarchaeota. They are classiﬁed on the basis of their 16S RNA sequence in ﬁve orders, Methano...

Ngày tải lên : 16/03/2014, 05:20

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Báo cáo khoa học: C ﬁ G base mutations in the CArG box of c-fos serum response element alter its bending ﬂexibility Consequences for core-SRF recognition potx

... Indeed, the change in the array of the hydrogen bonds at thymine of SRE GGfos is probably a sign of perturbation in the hydration scheme along the minor groove of the (A ⁄ T) domain. The G–C base pair ... strain There are several indications of a redistribution of the strains exerted on the oligonucleotide by the bend: partial unstacking of some aden...

Ngày tải lên : 07/03/2014, 09:20

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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... the glutamate that acts as the catalytic acid. The active site grooves of these chitinases are lined with aromatic amino acids that contribute to substrate binding [6,11]. Catalysis in retaining ... Family 18 chitinases are retaining glycoside hydrolases that have been found in many organisms varying from bacteria to humans [1,2]. The catalytic domains of family 18 chiti...

Ngày tải lên : 07/03/2014, 14:20

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Báo cáo khoa học: Aromatic amino-acid residues at the active and peripheral anionic sites control the binding of E2020 (AriceptÒ) to cholinesterases doc

... the indanone and the phenyl ring of E2020 is shorter than the distance between the active- site and the peripheral anionic site, E2020 can either bind at the active site or at the peripheral anionic ... the active- site and the peripheral ani- onicsiteinAChE,butinthecaseofBChE,asthegorgeis larger, E2020 cannot simultaneously interact at both sites. The observa...

Ngày tải lên : 30/03/2014, 20:20

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Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

... Invitrogen (Burlington, Canada); Oil Red O, Harris hematoxylin, polyornithine and laminine from Sigma (Oakville, Canada); formalin from Fisher Scientiﬁc (Nepean, Canada); Alamar blue reagent from ... were then removed and placed into scintillation vials containing scintillation liquid (ScintiSafe Plus 50%, Fisher Scientiﬁc Canada, Ottawa, Canada). Radioactivity was counted 24 h later, using...

Ngày tải lên : 07/03/2014, 00:20

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Báo cáo khoa học: Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase docx

... simulation of the EPR spectra of reacting holoenzymes, it was suggested that that the distances between Co(II) of cob(II)alamin and substrate radicals are  11 A ˚ in ethanolamine ammonia-lyase ... adeninylpropylcobalamin and other longer chain homologues do not [32]. These lines of evidence suggest the presence of adenine-binding site in the apo- enzyme and that...

Ngày tải lên : 07/03/2014, 21:20

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Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt

... play a vital role in providing acetyl-CoA and oxaloacetate in the cytosol as starting materials for a variety of biosynthetic pathways. Rat and human ACLs from various organs and tissues have ... Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase Tadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi and Tadayuki Imanaka Department of Synt...

Ngày tải lên : 08/03/2014, 22:20

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Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf

... cleavage site in the MCA substrate. Extended incubation and examination of the products of Fig. 2. Percentage activity of wild-type TOP with the substrates MCA, mcaBk, mcaGnRH 1–9 and mcaNt in the ... (CACCTCACTCCACAAGTAAGCATAGT ACTGAGCGTCGTA); FwRepG60 3A (CTTTTGGCCA CCTCGCTGCTGGCTACGACGCTCAGTAC); RvRepG- 60 3A (GTACTGAGCGTCGTAGCCAGCAGCGAGGTG GCCAAAAG); FwRepG60 4A (G...

Ngày tải lên : 23/03/2014, 06:20

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Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the speciﬁcity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

... linear kine- tics range. Mechanistically, Fpg and OGG1 differ in their ability to catalyze cleavage of the apurinic ⁄ apyri- midinic (AP) site via elimination of its 3¢-phosphate (AP lyase activity) ... assay endpoints were used in this case: glycosylase activity was measured after full thermal degradation of the AP site left by base excision, whereas the AP lyase activity...

Ngày tải lên : 18/02/2014, 18:20

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