Báo cáo khoa học: Localization of fluorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum pot

... expression. The status of the switch is determined by the state of the upstream regulatory element of the GAL genes in the nucleus. The regulatory protein may bind to the upstream regulatory element constituting ... could influence the binding of the regulatory protein to the other, representing cooperativity [5,6]. A regulatory protein may be the product o...

Ngày tải lên: 07/03/2014, 16:20

... SDS/PAGE. To detect the shift of the 21 kDa band, 2.5 mU of N-glycanase was added to 4 lg (56 pmol) of ZPA. To detect the shift of the 63 kDa band, 0.25 mU of N-glycanase was added to 1 lg (14 pmol) of ... glycosylated. Determination of the number of N-glycosylation sites We then investigated the number of N-glycosylation sites and the localization o...

Ngày tải lên: 21/02/2014, 03:20

... hydrolase of A. caldus (Eur. J. Biochem. 271) 275 Chemical stability of potassium tetrathionate the substrate of tetrathionate hydrolase To quantitate the substrate using the HPLC method, the effect of ... stable under these conditions at temperatures of up to 80 °C(data not shown). The interference of ammonium sulfate with the HPLC- assay of tetrathionate was al...

Ngày tải lên: 07/03/2014, 14:20

... on the amount of the probes injected, the phase of the cell cycle, and the position of the covalent linkage of the dye to the polymer. Results were indistinguishable for the low (white) and the ... Localization of fluorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum Miachael Karl 1 , Bernd Gas...

Ngày tải lên: 17/03/2014, 10:20

... d 1 heme-binding domain of cytochrome cd 1 . Therefore, we tested for the binding of d 1 heme to purified NirF. The addition of d 1 heme to the NirF resulted in the appearance of a distinctive visible ... NirF and a range of nucleotide-containing cofactors by using a variety of biophysical methods (data not shown). Nonetheless, we still decided to test the effect o...

Ngày tải lên: 15/02/2014, 01:20

... mini-receptors was measured to assess whether the presence of the mutant mini-recep- tors affected PMP-BSA binding to the wild-type mini- receptors (Fig. 5C). In these experiments, the null hypothesis ... domain but, rather, there are interactions that exist between dimer partners all along the ectodomain of the receptor. Collectively, these studies led to the hypothes...

Ngày tải lên: 18/02/2014, 08:20

... were attached on top of or inside the fibrillar 3D matrix (data not shown). Some of the synthesized MMP-2 was adsorbed to the fibrillar collagen, and the ratio of active to total MMP-2 was the same as ... surface of the cell that pro- duced them. The ECM can be found in different forms, from the hard compositions of bone to the soft structures of connective t...

Ngày tải lên: 18/02/2014, 11:20

... reduction of the ribosyl side chain of compound 3 in the ascomy- cete Ashbya gossypii is conducive to introduction of a hydrogen atom into the 1¢-proS position of the ribityl side chain of riboflavin ... carbon atoms of the ribosyl side chain [12]. Moreover, the signals of the side- chain carbon atoms of 13 C-labelled compound 3 appear as multiplets due to 13...

Ngày tải lên: 18/02/2014, 18:20

... initially to the a domain, thereby lead- ing to formation of the M 4 a cluster prior to formation of the M 3 b cluster, demonstrating that the individual binding constants of divalent metal ions for the a ... in efforts to elucidate the potentially cooper- ative nature of the metal-binding reaction within each of the domains. Thus, the goal is to elucidat...

Ngày tải lên: 19/02/2014, 00:20

... and the ability of the particular p53 target site to accommodate the cisplatin IACs. The higher the probability of formation of the cisplatin IACs within the p53DBSs due to the occurrence of the ... within p53DBS of the competitor but not within the p21b target; see (ii)]. The graphs show the relative binding of p53 to the radiolabeled targets as...

Ngày tải lên: 19/02/2014, 05:20