Báo cáo khoa học: The N-terminal cysteine pair of yeast sulf

Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

... The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre Go¨ tz Hofhaus 1 , Jeung-Eun ... the systematic functional analysis of the six cysteines in yeast Erv1p was the major goal of this paper. All three pairs of cysteines in Erv1p are indispen...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx

... 18–28) enzymes. The high degree of identity in the N-terminal sequences of these two proteins (Fig. 3A) suggests that the docking of the N-terminal fragment into the C-terminal region is different between the ... and C-terminal regions of CPT1A [9]. In this model, Asp17 does not face the C-terminal fragment. A possible explanation for this is that, in...

Ngày tải lên : 16/03/2014, 04:20

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Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

... been documented in a variety of tumors. Therefore, EGFR-tyrosine kinase is a promising therapeutic target. Although in vitro and in vivo studies have shown the anti-tumor activity of EGFR-tyrosine ... autophosphorylated independent of EGF. In this study, we investigated the signaling route by which the EGFR tyrosine kinase inhibitor AG1478 induces apoptosis in PC...

Ngày tải lên : 18/02/2014, 13:20

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Báo cáo khoa học: A Caenorhabditis elegans model of orotic aciduria reveals enlarged lysosome-related organelles in embryos lacking umps-1 function potx

... expres- sing GFP, and therefore having inherited the UMPS-1::GFP array, were scored for intestinal vacuoles. h Of the single mutants ⁄ RNAi exam- ined in the double mutant analysis, only umps-1()) single ... phosphatidylinositol-3-kinase that catalyzes the for- mation of phosphatidylinositol 3,5-bisphosphate and is orthologous to PIKfyve in mammals and Fab1p in...

Ngày tải lên : 06/03/2014, 09:22

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Tài liệu Báo cáo khoa học: Post-translational modiﬁcation of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... uptake in the early phase of invasion, con- sistent with the involvement of the microtubule system and GTPases in this process [32]. Once YpkA is present in the infected host cell, further active ... entry point to explain this effect. We therefore aimed to validate this result and examine whether this interaction also occurs during bacterial invasion in living cel...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: Post-translational modiﬁcations of the linker histone variants and their association with cell mechanisms docx

... although there is a good degree of agree- ment about the overall parameters of the fibre formed by folding the zig-zagging chain of nucleosomes in inactive chromatin, the location of the linker histone in ... respectively. Although the overall number of these modifications is low, the density of modifications is much the same as in the rest of the...

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Suppressed catalytic efﬁciency of plasmin in the presence of long-chain fatty acids Identiﬁcation of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt

... plasmin activity (e.g. 1 nm plasmin) was superimposed on the initial phase of the optical changes in Fig. 1A and these can be misinterpreted as an activating effect of oleic acid. Therefore, in the present ... 8B). These results preclude the catalytic domain as a target of the fatty acids and support the notion that interaction with kringle 5 is sufﬁcient for...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Speciﬁc membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc

... structure of the complex of I C with CPY (I C –CPY) [8,9] have provided information on the nature of the inhibition. The N-terminal acetyl group of I C is essential for inhibitory function, and the inhibitor ... [16], and little information is available regard- ing the phospholipid and membrane binding of the other members of the PEBP family. Cons...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khóa học: Non-speciﬁc depolymerization of chitosan by pronase and characterization of the resultant products pptx

... bead characteristics, in which increases in molecular mass and DA resulted in efﬁcient loading and minimization of the release of entrapped lipase [30]. The observed increase in speciﬁc activity with increasing ... 3600–3000 cm )1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement of both -OH… 3and -...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Mg2+-modulated KMnO4 reactivity of thymines in the open transcription complex reﬂects variation in the negative electrostatic potential along the separated DNA strands ppt

... which is clearly seen as band doubling for T)11 and T+2. Therefore, the measured contribution of these forms to the integrated intensity of these two bases was used in the calculation of the contribution ... quantiﬁcation of band intensities Images of footprints were obtained with the use of a Molecular Dynamics Phosphorimager. Integrated intensities of bands...

Ngày tải lên : 19/02/2014, 18:20

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