Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

... The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre Go¨ tz Hofhaus 1 , Jeung-Eun ... the systematic functional analysis of the six cysteines in yeast Erv1p was the major goal of this paper. All three pairs of cysteines in Erv1p are indispen...

Ngày tải lên: 17/03/2014, 10:20

... 18–28) enzymes. The high degree of identity in the N-terminal sequences of these two proteins (Fig. 3A) suggests that the docking of the N-terminal fragment into the C-terminal region is different between the ... and C-terminal regions of CPT1A [9]. In this model, Asp17 does not face the C-terminal frag- ment. A possible explanation for this is that, in...

Ngày tải lên: 16/03/2014, 04:20

... been documented in a variety of tumors. Therefore, EGFR-tyrosine kinase is a promising therapeutic target. Although in vitro and in vivo studies have shown the anti-tumor activity of EGFR-tyrosine ... autophosphorylated independent of EGF. In this study, we investigated the signaling route by which the EGFR tyrosine kinase inhibitor AG1478 induces apoptosis in PC...

Ngày tải lên: 18/02/2014, 13:20

... expres- sing GFP, and therefore having inherited the UMPS-1::GFP array, were scored for intestinal vacuoles. h Of the single mutants ⁄ RNAi exam- ined in the double mutant analysis, only umps-1()) single ... phosphatidylinositol-3-kinase that catalyzes the for- mation of phosphatidylinositol 3,5-bisphosphate and is orthologous to PIKfyve in mammals and Fab1p in...

Ngày tải lên: 06/03/2014, 09:22

... uptake in the early phase of invasion, con- sistent with the involvement of the microtubule system and GTPases in this process [32]. Once YpkA is present in the infected host cell, further active ... entry point to explain this effect. We therefore aimed to validate this result and examine whether this interaction also occurs during bacterial invasion in living cel...

Ngày tải lên: 16/02/2014, 15:20

... although there is a good degree of agree- ment about the overall parameters of the fibre formed by folding the zig-zagging chain of nucleosomes in inactive chromatin, the location of the linker histone in ... respectively. Although the over- all number of these modifications is low, the density of modifications is much the same as in the rest of the...

Ngày tải lên: 18/02/2014, 08:20

... plasmin activity (e.g. 1 nm plasmin) was superimposed on the initial phase of the optical changes in Fig. 1A and these can be misinterpreted as an activating effect of oleic acid. Therefore, in the present ... 8B). These results preclude the catalytic domain as a target of the fatty acids and support the notion that interaction with kringle 5 is sufficient for...

Ngày tải lên: 18/02/2014, 17:20

... structure of the complex of I C with CPY (I C –CPY) [8,9] have provided information on the nature of the inhibition. The N-terminal acetyl group of I C is essential for inhibitory function, and the inhibitor ... [16], and little information is available regard- ing the phospholipid and membrane binding of the other members of the PEBP family. Cons...

Ngày tải lên: 19/02/2014, 05:20

... bead characteristics, in which increases in molecular mass and DA resulted in efficient loading and minimization of the release of entrapped lipase [30]. The observed increase in specific activity with increasing ... 3600–3000 cm )1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement of both -OH… 3and -...

Ngày tải lên: 19/02/2014, 12:20

... which is clearly seen as band doubling for T)11 and T+2. Therefore, the measured contribution of these forms to the integrated intensity of these two bases was used in the calculation of the contribution ... quantification of band intensities Images of footprints were obtained with the use of a Molecular Dynamics Phosphorimager. Integrated intensities of bands...

Ngày tải lên: 19/02/2014, 18:20