Báo cáo khoa học: Choline increases serum insulin in rat when injected intraperitoneally and augments basal and stimulated aceylcholine release from the rat minced pancreas in vitro pptx

... Choline increases serum insulin in rat when injected intraperitoneally and augments basal and stimulated aceylcholine release from the rat minced pancreas in vitro Yesim Ozarda ... acetylcholine release from the minced pancreas. Effect of HC-3 on choline- induced increases in acetylcholine release from the minced pancreas...

Ngày tải lên: 17/03/2014, 09:20

... interpret- ation is in agreement with results obtained in murine adipocytes and in vivo in ADRP-deficient mice show- ing that there was no significant effect of adipophilin on basal and isoproterenol -stimulated ... with NaCl ⁄ P i and incubated in NaCl ⁄ P i containing 5% BSA for 30 min. For immunostaining, the slides were incubated 1 h with guinea- pig polyclonal antibodi...

Ngày tải lên: 23/03/2014, 10:21

... )1320/)274. The PCR products representing this promoter fragment was sub- cloned into PCR2.1 and transferred into pGL2-Basic using the KpnIandXhoI sites in the vector and KpnIandSalIsites in the PCR2.1 ... restriction fragment extending 3.5 kb upstream from the introduced SalI site was excised by digestion with SalIandSmaI (utilizing the SmaI contained in the pBluesc...

Ngày tải lên: 20/02/2014, 11:20

... of MIH and CHH in the sinus gland during the moult cycle As individual SG from single crabs always contained almost identical profiles and quantities of peptides (preliminary experiments using pairs ... Receptor binding assays for MIH and CHH using 125 I-labelled ligands were performed using ÔdisplacementÕ or ÔsaturationÕ type protocols, but modified so that the concentration of...

Ngày tải lên: 21/02/2014, 00:20

... genes in the overall lesion removing process. In this study our aim was to determine the NER rates of different rat tissues and to determine whether these rates correlated with the proliferative ... extracts and monitored the repair of UV- irradiated BlueScript plasmid DNA incubated in them. We normalized the repair rates against the total amount of protein in the...

Ngày tải lên: 23/03/2014, 20:22

... Accumulation in rat hepatocytes and hepatic protein binding of quinine and quinidine. Accumulation of quinine and quinidine (5 l M ) in rat hepatocytes is expressed as the steady state ratio (C/M) ... inhibition in the two systems. To test this hypothesis, we measured the total concentration of quinine and quinidine in rat hepatocytes, as well as their free (non- p...

Ngày tải lên: 31/03/2014, 07:20

... 20 amino acid signal peptide and a 68 amino acid peptide belonging to the insulin family (Fig. 3C). If the signal peptide is eliminated and disulfide bridges are formed as in other insulin family ... development of the wing disks at the prepu- pal stages. It is interesting to note that bombyxin-II also stimulated the growth of the wing disks in the butterfly Precis c...

Ngày tải lên: 18/02/2014, 13:20

... some links with the second half-site. The main difference between SRE fos and SRE Gfos , on the one hand, and SRE GGfos , on the other hand, consists in the distinct strains acting on the helix ... bent and a linear form keeping the same average conformations. Thus, an increase in temperature displaces the equilibrium, increasing the amplitude of motion around the...

Ngày tải lên: 07/03/2014, 09:20

... be explained by the fact that protein binding can increase the overall concentration of fatty acids present in an aqueous environment by effectively decreasing the insoluble fraction [2]. Albumin, in ... that Acot2 is the thioesterase involved in the release of AA inside the mitochondria. Our model explaining how AA is released into the mitochondria also supports the c...

Ngày tải lên: 16/03/2014, 12:20

... b-catenin signaling pathway, via inhibition of the glycogen syn- thase kinase-3b (GSK3b) [3], and depletion of the intracellular inositol pool via the inhibition of various enzymes in the phosphoinositide ... cell lysis and western blot analysis of the levels of ATP synthase-b and the loading control a-tubulin. The intensity of the protein bands was evaluated by den- s...

Ngày tải lên: 23/03/2014, 09:20