Báo cáo khoa học: Regulation of pyruvate dehydrogenase complex activity in plant cells ppt

... is capable of reducing oxidant-induced mod- ifications of proteins during exposure of yeast cells to H 2 O 2 [101]. The ability of trehalose to reduce intracel- lular oxidation during dehydration ... high levels of soluble sugars. Although an involvement of metabolic signals in the regulation of plant senescence has been demonstrated in a range of studies [15, and ref...

Ngày tải lên: 15/03/2014, 11:20

... purification of the included volume of the column in (A), large O-glycans (j), obtained as the included volume of the column in (C), and small O-glycans (s), obtained as the included volume of the ... clear-cut results. Cells were transfected with a linearized plasmid containing a 553-bp fragment of b3Gal-T5 cDNA, that includes the initial 360 bp of the coding sequence and 19...

Ngày tải lên: 19/02/2014, 12:20

... [46]. Pro-caspases exist in living cells and casp- ases are indispensable for the proliferation of some cell lines. When deciding between maintaining cells alive or inducing apoptosis, caspase function ... cleavage fragments of caspase-3 ⁄ 7 sometimes exist in living cells, but are inactive due to the binding of XIAP [50]. Although chemical caspase inhibitors bind to caspase fr...

Ngày tải lên: 14/02/2014, 22:20

... genetic diseases [47]. Most analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene expression, have ... forced expression of Runx2 in nonosteoblast cells is sufficient to induce the osteoblast-specific gene osteocalcin [60]. The inactivation of both Runx2 alleles in mice resul...

Ngày tải lên: 16/02/2014, 09:20

... members of the HSF family are modular proteins composed of functional domains (see figure 2 in [4]). The most con- served domain is the amino-terminal helix-turn-helix DNA-binding domain (DBD). ... gene induction. During the attenuation phase the transactivation capacity of HSF1 is repressed through a negative-feedback loop via binding of HSPs. The DNA-binding activity of HSF1 is...

Ngày tải lên: 18/02/2014, 04:20

... mechanism in which the N-terminus of the inhibitor first binds to the enzyme, displacing the occluding loop, followed by the binding of another two loops of the inhibitor [24]. Besides protein inhibitors, ... 1.8 nm, depicting a strong interaction between 2A2 mAb and cathepsin B. Determination of the 2A2 mAb binding site on cathepsin B The binding site of 2A2 mAb on cathepsin B was...

Ngày tải lên: 18/02/2014, 11:20

... dCTP(dUTP)-binding conformer and the dTTP-binding conformer of dCTP deaminase. We were not able to identify structural changes in the main chain of the subunit, or in the interaction of subunits within ... substrate and inhibitor for binding to the same site [12]. We have previously determined the structures of wild-type dCTP deaminase in complex with dUTP and the inactive E13...

Ngày tải lên: 18/02/2014, 16:20

... studies indicate that regulation of PtdIns metabolism by R hoA GTPase is likely involved because the increase in PtdIns turnover often correlates with increase in F-actin levels within the cells ... and stained for F-actin with rhodamine-conju- gated phalloidin. Th e effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the cells with either 10...

Ngày tải lên: 19/02/2014, 16:20

... entire length of thin filaments and is capable of modulating the r ate of formation of the a ctin– myosin complex [74,75]. Interestingly, nebulin also interacts with the titin PEVK domain in a calcium/calmodulin ... modification of the actin–HMM interface, which results in the inhibition of HMM cross- linking to the N-terminal part of a ctin. This change of the actin–HMM i...

Ngày tải lên: 19/02/2014, 16:20

... iodide (PI) staining of apoptotic cells was quantified in VSV- infected Jurkat T cells in the absence or presence of spermine. As shown in Fig. 7, the presence of spermine during VSV infection strongly ... infection. ArgII is involved in the biosynthesis of natural poly- amines (putrescine, spermidine and spermine) through conversion of l-arginine into l-ornithine [16]. T...

Ngày tải lên: 07/03/2014, 21:20