Báo cáo khoa học: Interaction of caspase-3 with the cyclic G

Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt

... result of the strong binding of secondary anti-rabbit serum to the protein-A moiety of the TAP-tag. Anti-CBP serum was used to show the expression of the TAP-tag alone. 42Sp50 interacts with the RNA ... the presence of known localization factors. Input corresponds to 1% of the material used in the pulldown experiment. The presence of the TAP-Vg1RBP band...

Ngày tải lên : 23/03/2014, 03:20

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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... responsible for the actual membrane fusion step. However, the nature of the interaction of viral fusion proteins with membranes and the mechan- ism by which these proteins accelerate the formation of membrane ... lipid–peptide interaction. The presence of this Trp residue in W-E2(279–298) modiﬁed neither the hydrophobicity (0.16) nor the pI (6.14) of the paren...

Ngày tải lên : 07/03/2014, 17:20

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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx

... represent the limited case of six triplet sequences, the specificity of the observed effects confirms the higher selectivity of DAPI for the minor-groove of the ATT-triplet with respect to all the other individual ... attrib- uted to the (ATT) 10 region (located at the 5¢-end of the ATT-template) folding into a hairpin-like structure. The double helix of the...

Ngày tải lên : 16/03/2014, 23:20

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Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot

... shift in the position of the 208-nm peak (Fig. S1). Quantitative evaluation of the CD data is shown in the bar graph A B Fig. 3. Effect of RNA binding on the thermostability of UDE. (A) Thermal ... Characterization of the bound RNA pool by real-time RT-PCR revealed an abundance of UDE mRNA as well as of domain V of 23S rRNA. These results, together with the c...

Ngày tải lên : 22/03/2014, 16:21

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Báo cáo khoa học: Deletion of Phe508 in the first nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator increases its affinity for the heat shock cognate 70 chaperone docx

... quantify the binding of wt- and F508del-mNBD1. Assessment of the impact of F508del on the NBD1–Hsc70 interaction To determine the strength of the NBD1–Hsc70 interaction, we thus immobilized the ... previously proposed model of the ERQC, the folding states of CFTR are assessed within the ER at multiple checkpoints [9,10], the ﬁrst of these involving the...

Ngày tải lên : 23/03/2014, 05:22

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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... differently with HSP25 so we analyzed the effect of HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the ﬁrst series of experiments ... appearance of an additional peak at 7.65 mL on the elution proﬁle. Increase of the temperature of incubation was accompanied by the simultaneous increase...

Ngày tải lên : 20/02/2014, 23:20

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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP. On the other hand, 2-oxoglutarate did not inﬂuence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... either a stable soluble complex with GlnK in the absence of AmtB, or constitutively binds to GS in the absence of GlnK. In vitro, the TnrA C-terminus is responsi...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... mol ⁄ mol). The results are mean ± SD, n ¼ 1–4. The degree of permea- bilization is expressed as the percentage of the maximal value obtained at the end of the assay by the addition of 2 m M Triton X-100. ... study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and bilayers. Interaction of stefin B in the prefib...

Ngày tải lên : 07/03/2014, 17:20

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Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx

... systemically within the host plant [11]. The present study comprised biochemical characterization of the MP encoded by ORF1 of SeMV, a member of the genus Sobemovirus. Analysis of the deduced ... degree of interaction between the proteins remains. It is possible that another site of interaction beyond the N-terminal 49 residues may contribute to these weak inte...

Ngày tải lên : 15/03/2014, 00:20

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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx

... quantify the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a ﬁxed aliquot of the protein was incubated with 1 ng of the ... abundant, compatible with the binding of eEF1A in the CRC (white arrow), the other of apparent molecular mass of about 100 kDa compatible with bindin...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP speciﬁc phosphodiesterase (PDE5a1) potx

Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt

Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc