Báo cáo khoa học: Two CCAAT/enhancer binding protein sites in the cytochrome P4503A1 locus Potencial role in the glucocorticoid response ppt

... Two CCAAT/enhancer binding protein sites in the cytochrome P4503A1 locus Potencial role in the glucocorticoid response Elsa Rodrigues1, Marie-Jose´Vilarem2, ... shown).Characterization of the C/EBP binding sequences in the CYP3A1 promoter regionWe used gel mobility-shift assays, with the goal of identi-fying active C/EBPa binding sites within the CYP3A1 locus. First, ... 2003 Two C/EBP sites in the CYP3A1 locus (Eur. J. Biochem. 270) 5593 bp, did not. The results suggest the presence of two C/EBPa binding sites at positions )300 and )600 within the CYP3A1 locus. Activation...

Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx

... September2009)doi:10.1111/j.1742-4658.2009.07380.xPolypyrimidine tract -binding protein (PTB) is a widely expressed RNA- binding protein with multiple roles in RNA processing, including the splic-ing of alternative exons, mRNA ... Poly-pyrimidine tract -binding protein promotes insulinsecretory granule biogenesis. Nat Cell Biol 6, 207–214.10 Kosinski PA, Laughlin J, Singh K & Covey LR (2003)A complex containing polypyrimidine ... &Hardy S (2004) Polypyrimidine tract -binding protein isinvolved in vivo in repression of a composite inter-nal ⁄ 3¢-terminal exon of the Xenopus alpha-tropomyosinPre-mRNA. J Biol Chem 279,...

Báo cáo khoa học: Polypyrimidine tract binding protein regulates alternative splicing of an aberrant pseudoexon in NF1 pdf

... skipping was maintained upon PTBdepletion, indicating that variations in PTB binding are not the main reason for the skipping observed withdel_a and del_c, despite the strong increase in PTB binding ... mutant,suggesting that its binding might be responsible for the increased exon skipping in the del_a mutant. In con-trast, PTB showed reduced binding to del_c. Binding ofother well-known splicing factors, ... nuclear extract protein binding proﬁle of WT, del_a and del_c RNAs followingCoomassie staining. The boxed area shows the protein bands that display the greatest change in binding proﬁle. (D)...

Báo cáo khoa học: Cellular retinol-binding protein type II (CRBPII) in adult zebraﬁsh (Danio rerio) cDNA sequence, tissue-speciﬁc expression and gene linkage analysis pptx

... retinolmetabolism.Cellular retinol -binding proteins (CRBPs) are members of the intracellular lipid -binding protein family which includes the retinoic acid (CRABP) and fatty acid (FABP) binding proteins. ... mammals may provide insight into the role( s) ofthese intracellular retinol- and retinal -binding proteins in vitamin A metabolism. As part of ongoing studies in ourlaboratories on the evolution, ... geneexpression in the intestine appear therefore to have beenconserved for more than 400 million years.Keywords: Danio rerio; fatty acid binding protein; cellularretinol binding protein; tissue-speciﬁc...

Báo cáo khoa học: A sucrose binding protein homologue from soybean exhibits GTP-binding activity that functions independently of sucrose transport activity pptx

... whereas protein binding to ATP-agarose resin was negligible (lane 3). The S64/SBP2 recombinant protein also did not bind to protein A-agarose resin (lane 3). The speciﬁcity of S64/SBP2 binding to ... sheets [33,34]. In contrast, the sucrose binding protein shows no structural homology withother GTP -binding proteins, except for the presence of animperfect nucleotide binding A consensus Ala279-X-X-X-X-Lys-Lys-Ser286, ... FEBS 2002 GTP binding of sucrose binding protein (Eur. J. Biochem. 269) 4003previous association of S64/SBP2 with GTP -binding pro-teins present in the whole cell extracts. To examine thesepossibilities,...

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... signatures in the sequences are present in both proteins, tyrosinases andcatechol oxidases; these include the six histidine resi-dues involved in the binding of a pair of copper ionsand other conserved ... as shown in Fig. 7B. Binding of monophenols to resting met-tyrosinaseresults in the inactive dead-end complex, but binding of o-diphenols leads the enzyme to the oxy-tyrosinaseform, the active ... tyrosinases, including the CuA and CuB binding sites to ligand the copperTable 3. Kinetic parameters for the two PPOs. The enzymes wereobtained from extracts of R. solanacearum strains mutated in...

Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx

... single polyamines, mainly spermine, can modulate the DNA -binding properties of proteins, either increas-ing or diminishing their afﬁnity for DNA [42]. Thisability, to modulate the DNA -binding ... gen-erated by the high concentration of polyamines, whichdrives these cations into the nonpolar DNA phase,and to the decreased number of DNA -binding sites per polyamine, making the DNA more ... (between the sec-ondary amino groups of polyamines and purine-N7or thymine-O4 residues) [1,50]. The insertion of NAPmonomers into the DNA grooves forms the basis of the recognition process occurring...

Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf

... subunit -binding domains in B56aTo determine the minimal r egion of B56 that interacted with the PP2A subunit, we utilized an in vitro binding assay usingGST-A subunit and reticulocyte lysate-synthesized ... utations in distinct lo ops alter the binding of the B and C subunits [13]. The B subunitsbind to repeats 1±10 of the A subunit, whereas the C s ubunitbinds to repeats 11±15. Interactions between the ... seconddomain e xtending from amino acids 325 ±383 wa s capable ofindependently binding to GST-A ( Fig. 2). These regionswere named A subunit b inding domains (ASBD) 1 and 2.Given that the two distinct...

Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... chapero-nin, but in this case a strong MCAD protein band was seen in the westernblots throughout. The proteins were also puriﬁed, and the enzyme functionand thermostability investigated. The K364R protein ... on the west-ern blot and by the minimal effect of chaperonin(Fig. 1B). This suggests that the R256T mutationharms the function of the folded protein rather than the ability of the protein ... MCAD protein detected in the western blots (Fig. 1B).Comparison of activity levels of the mutantproteins, as determined by activity staining The apparent inactivity of R256T was further investi-gated...

Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf

... FEBSproteins, based on the 3D structure of maize poly-amine oxidase (MPAO), indicated that this region islocalized on the tip of the FAD -binding domain, in close spatial proximity to the protein ... amino acids, has not been shownto contain any residue involved in either the catalyticsite or the FAD -binding domain [9,10,12,13]. Interest-ingly, this 31 amino acid region is highly conservedamong ... mammalianfeature.These data suggest that the two domains NDA andNDB, not involved in enzyme activity or FAD bind-ing, could be responsible for the interaction with the nuclear targeting machine. With...

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