Báo cáo khoa học: Two CCAAT/enhancer binding protein sites in the cytochrome P4503A1 locus Potencial role in the glucocorticoid response ppt

... Two CCAAT/enhancer binding protein sites in the cytochrome P4503A1 locus Potencial role in the glucocorticoid response Elsa Rodrigues 1 , Marie-Jose ´ Vilarem 2 , ... shown). Characterization of the C/EBP binding sequences in the CYP3A1 promoter region We used gel mobility-shift assays, with the goal of identi- fying active C/EBPa binding sites wi...

Ngày tải lên: 17/03/2014, 09:20

... September 2009) doi:10.1111/j.1742-4658.2009.07380.x Polypyrimidine tract -binding protein (PTB) is a widely expressed RNA- binding protein with multiple roles in RNA processing, including the splic- ing of alternative exons, mRNA ... Poly- pyrimidine tract -binding protein promotes insulin secretory granule biogenesis. Nat Cell Biol 6, 207–214. 10 Kosinski PA, Laughlin J, Singh...

Ngày tải lên: 07/03/2014, 00:20

... skipping was maintained upon PTB depletion, indicating that variations in PTB binding are not the main reason for the skipping observed with del_a and del_c, despite the strong increase in PTB binding ... mutant, suggesting that its binding might be responsible for the increased exon skipping in the del_a mutant. In con- trast, PTB showed reduced binding to del_c. B...

Ngày tải lên: 16/03/2014, 04:20

... retinol metabolism. Cellular retinol -binding proteins (CRBPs) are members of the intracellular lipid -binding protein family which includes the retinoic acid (CRABP) and fatty acid (FABP) binding proteins. ... mammals may provide insight into the role( s) of these intracellular retinol- and retinal -binding proteins in vitamin A metabolism. As part of ongoing studies in our...

Ngày tải lên: 17/03/2014, 11:20

... whereas protein binding to ATP-agarose resin was negligible (lane 3). The S64/SBP2 recombinant protein also did not bind to protein A-agarose resin (lane 3). The specificity of S64/SBP2 binding to ... sheets [33,34]. In contrast, the sucrose binding protein shows no structural homology with other GTP -binding proteins, except for the presence of an imperfect nucleotide...

Ngày tải lên: 23/03/2014, 21:21

... signatures in the sequences are present in both proteins, tyrosinases and catechol oxidases; these include the six histidine resi- dues involved in the binding of a pair of copper ions and other conserved ... as shown in Fig. 7B. Binding of monophenols to resting met-tyrosinase results in the inactive dead-end complex, but binding of o-diphenols leads the enzyme to...

Ngày tải lên: 19/02/2014, 07:20

... single polyamines, mainly spermine, can modulate the DNA -binding properties of proteins, either increas- ing or diminishing their affinity for DNA [42]. This ability, to modulate the DNA -binding ... gen- erated by the high concentration of polyamines, which drives these cations into the nonpolar DNA phase, and to the decreased number of DNA -binding sites per polyamine, making...

Ngày tải lên: 23/03/2014, 15:20

... subunit -binding domains in B56a To determine the minimal r egion of B56 that interacted with the PP2A subunit, we utilized an in vitro binding assay using GST-A subunit and reticulocyte lysate-synthesized ... utations in distinct lo ops alter the binding of the B and C subunits [13]. The B subunits bind to repeats 1±10 of the A subunit, whereas the C s ubunit binds t...

Ngày tải lên: 08/03/2014, 10:20

... chapero- nin, but in this case a strong MCAD protein band was seen in the western blots throughout. The proteins were also purified, and the enzyme function and thermostability investigated. The K364R protein ... on the west- ern blot and by the minimal effect of chaperonin (Fig. 1B). This suggests that the R256T mutation harms the function of the folded protein rat...

Ngày tải lên: 20/02/2014, 02:21

... FEBS proteins, based on the 3D structure of maize poly- amine oxidase (MPAO), indicated that this region is localized on the tip of the FAD -binding domain, in close spatial proximity to the protein ... amino acids, has not been shown to contain any residue involved in either the catalytic site or the FAD -binding domain [9,10,12,13]. Interest- ingly, this 31 amino acid re...

Ngày tải lên: 07/03/2014, 17:20