Báo cáo khoa học: The transthyretin-related protein family doc

... mixing together HIS-OXI1 and GST-PTI1-4 proteins or HIS-PTI1-4 and GST-OXI1 proteins, the GST-tagged proteins were pulled down with glutathi- one-agarose beads. The proteins were then detected ... PTI1-4, another member of the PTI1-like family, interacts with OXI1. By using yeast two-hybrid assays, other members of the AGC family (AGC2-2 and AGC2-3) were shown to interact with the...

Ngày tải lên: 14/02/2014, 19:20

... independent proteins and peptides leads to the disassembly of the spherical capsid inside the virion. Some of the folded domains of Gag are then able to reassemble as inde- pendent proteins. The matrix protein ... involved either in the assembly of the immature HIV-1 capsid, or in the reassembly of CA into a mature capsid. The detailed description of the structure and co...

Ngày tải lên: 18/02/2014, 06:20

... polyprotein, yields the matrix protein (MA), the capsid protein (CA), the nucleocapsid protein (NC) and p6 proteins, as well as two spacer pep- tides. Currently, most of the available drugs in the ... folding of both the CAI peptide and a-helix 9 of CTD during formation of the peptide– protein and protein protein complexes. These NMR experiments also allowed the dete...

Ngày tải lên: 18/02/2014, 06:20

... to the nAChR has also been reported for free fatty acids [25] and local anaesthetics [26]. The nAChR is the by far best-characterized mem- ber of the superfamily of ligand-gated ion channel proteins ... [10]. The curve fits to the data points for tadpole narcosis by the additional alcohols of Table 1 are shown in Fig. 2 along with the theoretical curves for the enhancement of...

Ngày tải lên: 19/02/2014, 18:20

... anti-T7) was then used to monitor the location of the epitope-tagged region with respect to the KdpD protein in the membrane. The KdpD protein with the epitope tags was readily digested by proteinase ... and was not digested by the protease. Taken together, these results suggest that the second periplasmic loop of the wild-type KdpD protein is inserted into the membr...

Ngày tải lên: 20/02/2014, 11:20

... humans. The RNA genome of the virus encodes a Gag polyprotein, which is further processed into matrix, capsid and nucleocapsid proteins. These proteins play a significant role at several steps in the ... 6118–6127 ª 2009 The Authors Journal compilation ª 2009 FEBS contains the viral RNA genome coated by the NC protein. The mature virion can then infect other host cells. Along ea...

Ngày tải lên: 07/03/2014, 00:20

... demonstrating: (a) the inability of the mutant to insert a cofactor, and (b) the specific origin of the signal in the other samples. The cytosolic fraction of the complemented wild-type clearly showed the EPR ... between the total activity in the nonfractionated cell lysate and the sum of the individual fractions can be easily explained by either loss of material or pr...

Ngày tải lên: 07/03/2014, 11:20

... porter.stem(token), stem is excit The corpora included with NLTK come with corpus readers that understand the file structure of the corpus, and load the data into Python data structures. For example, the following ... within the confines of a single-semester course, either at advanced undergraduate level or at postgradu- ate level. Unfortunately, it turns out to be rather difficult t...

Ngày tải lên: 20/02/2014, 12:20

... restrictions, the creation of a treebank faces a tradeoff between linguistic richness and treebank size. The richer the annotations, the slower the annotation process, the smaller the re- sulting ... This procedure and the size of the batches was determined to be optimal for both the software and the annotators’ productivity. To track the annotation quality, several ke...

Ngày tải lên: 17/03/2014, 02:20

... the TTR-related protein from the Gram-negative bacterium E. coli .The construct including the signal sequence generates two protein products and MS confirms that one corresponds to the mature protein ... and the other represents the processed mature protein, which proves that the signal sequence was cleaved after Ala23 as predicted. In all subsequent experiments the con...

Ngày tải lên: 17/03/2014, 09:20