... Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids Kohsuke Honda, Michihiko Kataoka, Eiji Sakuradani and Sakayu Shimizu Division ... 3,4-dihydro-coumarin hydrolase; perhydrolase; peroxoacid. In a previous study, we found and isolated a novellactonohydrolase, 3,4-dihydrocoumarin hydrolase (DCH),from Acinetobacter calcoaceticus ... vector, and pKT231 [19]was used for construction of the dch disruptant.Enzyme assay, SDS/PAGE and protein determination Acinetobacter strains and the recombinant E. coli strainswere cultured in...