Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx

... blocked the stimulation of DNA and RNA synthesis, prevented smooth muscle cell migration and suppressed the induction of c-fos and c-myc gene expression. An examination of relevant signal transduction ... MAP kinase, Rho mediates growth factor-dependent activation of c-fos via the serum response factor (SRF) [30]. Protein kinase C-related kinase 1 (PRK1...

Ngày tải lên: 17/03/2014, 09:20

... mutants of the implicated residue in two template enzymes. Differences in the reactivity of E2020 toward AChE and BChE and a comparison of K I values of E2020 for mouse (Mo) AChE mutants of Trp86(84), ... ammonium group of E2020 and the c-oxygen of Asp74 which are separated by a distance of 5.4 A ˚ ;(c)ap–p interaction between the indanone ring of E2020 and Trp286 in...

Ngày tải lên: 30/03/2014, 20:20

... Apoptotic cells were identified by the condensation and fragmentation of their nuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells ... protein expression and mRNA stability in the presence or absence of H 2 O 2 . (A) Western blot analyses showing the effect of nucleolin ⁄ C23 on Bcl-2 protein expr...

Ngày tải lên: 16/02/2014, 09:20

... members of the Ras family of small G proteins. The activity of Ras proteins is under tight control of several classes of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins. ... 5-HT 7 receptors induced protein kinase A-dependent phosphorylation of the endogenous human Ras-GRF1 on Ser927 and of transfected mouse Ras-GRF1 on Ser916. Ras-GRF1 overex...

Ngày tải lên: 19/02/2014, 18:20

... atom types and Gasteiger atomic charges were assigned to the ligand atoms [59]. We limited the output to a maximum of 200 placements per ligand; the (R) and (S) enantiomers of NMNorsal and NMSal ... °C. Phosphorylation by protein kinase A facilitates the release of bound catecholamines and is the most prominent mechanism of tyrosine hydroxylase reactivation. Protein kin...

Ngày tải lên: 07/03/2014, 05:20

... the 3¢-UTR of uPA and of c-met mRNAs and translationally repress the expression of uPA and c-met in HCC cells. The evidence obtained shows that overexpression of miR-23b leads to uPA and c-met ... 3 and 6, and lanes 2 and 5, Fig. 3. miR-23b inhibits uPA and c-met protein expression in SKHep1C3 cells. (A) Western blot analysis of uPA in the conditioned media of...

Ngày tải lên: 29/03/2014, 23:20

... high incidence of teratomas and resulted in a failure of germ cells to arrest mitosis [56]. Thus, Dmrt1 in mice and similarly Dmrt1bY in medaka appear to be regu- lators of germ cell proliferation. Conclusion To ... germline expression is dispensable, condi- tional dmrt1 inactivation in either the Sertoli cells or the germ cells indicated that mouse Dmrt1 is indeed required fo...

Ngày tải lên: 14/02/2014, 19:20

... (pcDNA3.0 alone) or wild-type cells (Fig. 1A). Protein expression of HSS in the three cell lines (untransfected cells, vector-transfected cells, and HSS-expressing cells) was detected using western blot. ... in cirrhotic human livers, and the mRNA level of HSS was elevated in tissue samples of hepatocellular carci- noma and cholangiocellular carcinoma [5]. This increased ex...

Ngày tải lên: 16/02/2014, 09:20

... case of cell samples without coexpression of GAPDH) or from the cytoplasmic area (in the case of cell samples with coexpression of GAPDH) of cells, and are expressed as mean ± standard deviation. ... 1. Subcellular localization and enzymatic activities of GAPDH– citrine and PGK–cerulean. (A) Expression of GAPDH–citrine and PGK–cerulean. Localization of endogenou...

Ngày tải lên: 16/02/2014, 09:20

... transcription via activation of P-TEFb kinase. In line with this, after 5 days of induction, ectopic expression of AF4–MLL resulted in the transcriptional deregulation of 660 genes, of which 580 ... several other proteins. Thus, the pattern of proteins bound to both reciprocal MLL proteins is quite complex and will influence the biological properties of a given MLL fusion...

Ngày tải lên: 16/02/2014, 14:20