Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx

... modules. The binding site for protein S mainly involves the first of the three b-chain CCPs (CCP1). However, recently it has been suggested that CCP2 of the b-chain also contributes to the binding of ... CCP2 had no effect on binding of protein S The b-chain constructs were tested in three different assays for their ability to bind protein S. I...

Ngày tải lên: 17/03/2014, 09:20

... liver lysates was measured using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer s instructions. Bovine serum albumin was used as standard to ... polymorphisms. This confirms our hypothesis that the effect of the Trp316Ser polymorphism was due to its non-random association with the )1CfiA mutation and that the effect of...

Ngày tải lên: 31/03/2014, 07:20

... mechanisms and homologous E3 ubiquitin ligases are involved in both cases. On the basis of structural studies, Lys147 is a solvent-accessible residue located far from the GTP -binding site, is not involved ... Each of the 18 lysine mutants of the myc–Rac1L61 sequence and the ubiquitin mutants UbR48 and UbR63 were obtained by replacing the lysine codons AAA and A...

Ngày tải lên: 18/02/2014, 16:20

... exacerbated by the fact that the H-domains of signal peptides lack lysine residues, which are required for cross-linking with the lysine-specific reagent DSS. Nevertheless, binding of B. subtilis Ffh ... localization of proteins in B. subtilis was determined by protoplasting and subsequent trypsin accessi- bility assays. Protoplasts were prepared from B. subtilis cells i...

Ngày tải lên: 16/03/2014, 22:20

... CD163. In humans and animals, infections or in ammatory stimuli cause changes in the activities and expression levels of various forms of CYP in the liver. In most cases, CYPs and their activities ... he- patocytes [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxida...

Ngày tải lên: 14/02/2014, 18:20

... importance of these interactions remains unclear and needs to be investigated in future studies. Concluding remarks H. pylori represents one of the most successful human pathogens, inducing severe clinical ... length restrictions of this minireview. The work of S. W. was supported by the Deutsche Forschungsgemeinschaft (We2843 ⁄ 2-1) and BGAG-Stiftung Walter Hesselbach....

Ngày tải lên: 14/02/2014, 19:20

... cotransfected pRL-SV40 control plasmid. Statistics Results are expressed as the mean ± SD for each sample. Statistical analysis was performed using the unpaired Student s t -test. Statistical significance ... LD inhibited the increase in Cer levels induced by serum starvation stress. These findings suggest that regulation of Cer levels is involved in enhanced cell survival result...

Ngày tải lên: 18/02/2014, 18:20

... lysates confirmed the absence of cathepsin D in nontransfected cells, and the presence of both the 31 kDa mature cathepsin D and the 45 kDa procathepsin D in cathepsin D-overexpressing cells (Fig. 6A, ... whose endogenous cathepsin D expression was inhibited by small RNA-mediated gene silencing (cathepsin D siRNA) (Fig. 6B). A progressive decrease in expres- sion of b...

Ngày tải lên: 19/02/2014, 02:20

... is involved in cell survival through downstream signaling cascades such as the MAP kinase and phosphatidyl- inositol-3¢-OH kinase (PtdIns3-K) ⁄ Akt pathways. These signals in uence survival through several ... Because caspase 9 is intimately associated with the initiation of apoptosis, EGF seems to exert its protective action against ADR-induced apoptosis by suppressing caspase 9...

Ngày tải lên: 19/02/2014, 06:20

... tyrosine-phosphorylated proteins first. We show here that one of these proteins, DPP IV, is tyro- sine-phosphorylated in a ligand-dependent manner. Results MS analysis of major proteins purified by ... overproduc- tion is associated with attenuated hepatic insulin signal- ing and overexpression of protein- tyrosine phosphatase 1B in a fructose-fed hamster model of insulin resis...

Ngày tải lên: 19/02/2014, 07:20