Báo cáo khoa học: Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana pptx
Báo cáo khoa học: Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana pptx
... SDS-gelelectrophoresis of the Blue Sepharose column fraction(Fig. 4A) and agrees with the molecular mass of c-TMT from A. thaliana (Fig. 4B). The presence of only onelabelled band is also indicative ... detailed biochemical characterization of a c-tocopherol methyltransferase (c-TMT) from Arabidopsis thaliana and of a c-TMT purified from Capsicum annuum fruits, a tissue with high accumulation of ... Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana Maria Koch1,2, Rainer Lemke3, Klaus-Peter Heise2 and Hans-Peter Mock11Institute of...
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Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle ppt
... thiosulfatophilum alsoharbors the same levels of malate dehydrogenase(0.62 UÆmg)1[1]), previous radiolabeling experiments dem-onstrated a large accumulation of oxaloacetate in the cells,relative ... values from 8.0 to 9.0, and Chesbuffers with pH values f rom 8 .7 to 10.0 were used for theassay.RESULTSIsolation of theidhgene from C. limicolaIn the cell-free extract of C. limic ola ... E. coli BL21(DE3), and the recombinant cellswere cultured in Luria–Bertani medium containing50 lgÆmL)1ampicillin at 37 °C. Expression of idh in therecombinant cells under the control o f...
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Báo cáo khoa học: Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity pdf
... each lane and analyzed on16% polyacrylamide gels using Coomassie Blue stain-ing. Lane S1, E. lagascae SCP2 Lys28Glu; lane S2,E. lagascae SCP2 Leu99Met; lane S3, E. lagascaeSCP2 Leu13Met ⁄ Leu14Met; ... Leu14Met; lane S4, A. thaliana SCP2Met100Leu; lane S5, A. thaliana SCP2 Met14Leu ⁄Met15Leu ⁄ His18Phe; lane S6, E. lagascae SCP2 wild-type; lane S7, A. thaliana SCP2 wild-type; lane M,PageRulerÔ ... compilation ª 2006 FEBSacid (DMPA). Ergosterol, sitosterol and cholesterolonly showed a marginal competing effect, and sterylglucoside, trimyristin, monogalactosyldiacylglycerol,galactosylceramide...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... and signal transduction. The role of PS in c-secretase activityis compelling and includes lack of Ab amyloid peptidegeneration and Notch signalling in PS double knockoutcells, cofractionation of ... were obtained from the NHMRC Tissue Resource Centre (Melbourne,Australia) and were well characterized pathologically and clinically [23]. Details of pathology or mutation analysis of tissue used ... CTF, low level of full-length PS1, mature NCT, and PEN-2 (Fig. 4F). PS1 CTF was detected with PS1 loopantibody as well as low amounts of full-length PS1. Theepitope for this antibody is largely...
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Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redefinition of the I-conotoxin superfamily pot
... well 4. Segments of muscle in wells 2 and 3 were kept submerged by a hold-down pin protruding horizontally from the wall of eachwell and overlying the muscle. Exposed portions of themuscle ... Bulaj1, Elsie C. Jimenez1,3 and Baldomero M. Olivera11 Department of Biology and 2 Pathology, University of Utah, Salt Lake City, UT 84112, USA3 Department of Physical Sciences, College of ... respectively. Henceforth,we will refer to the d version of the peptides simply asr11b and r11c, and the all -l versions as [l- Phe44]r11b and [l- Leu42]r11c.Increased biological potency of D-amino-acid-containing...
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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc
... signals were monitored in parallelwith cell growth and after cell cycle arrest due toirradiation.Cell proliferation and cell cycleCell growth behaviour was examined in HeLa and MCF-7 cells. ... from terminal methyl and bulk methylene coupling in ML. The reference cross peak from Lys is also labelled. Label T refers to the glycerol crosspeak of triglycerides. The insert shows the glycerol ... the1H NMR MLsignals of cultured tumour cells, specifically HeLa and Keywordscell cycle; cell metabolism; lipids; magneticresonance spectroscopy; tumour cell linesCorrespondence L. Guidoni,...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... epithelial and conjunctivecells of the mantle.Cg-Clp1 and Cg-Clp2 mRNA levels are increasedin haemocytes after bacterial LPS challengesAs the two mammalian CLPs, YM1 and YKL-40,were recently ... FEBSQuantification of mRNA levels in haemocytesafter bacterial LPS challengeIn vivo challengeAnimals were injected with 100 lg (in 100 lL of NaCl ⁄ Pi) of Escherichia coli 026:B6 LPS (Sigma-Aldrich) ... Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal regions share relatively low levels of sequence identity (46%), probably as the result of alower pressure of selection during evolution. Neverthe-less, these...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... QGPI cells0 1lucluclucluclucluclucluclucluc2pGL3-Basic Vector−1995H−2081H−1331H−524H−197HmS−94H−73H−60H−45H−14Hluc−197H−11871−11781Fig. 4. Summary of relative luciferase ... Irrespective of a lack of alteration inlevels of other conventional cardiac markers such ascreatine kinase isoenzyme MB and cardiac troponin T,serum DNase I levels rose significantly from basal lev-els ... cells and pancreas seem to arise from alternat-ive splicing. The complex patterns of alternativelyspliced products are represented schematically inFig. 2B. Although the splicing patterns of...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... isopropyl thio-b-d-galactoside (IPTG). After 1 h of induction, cells were cooled on ice, centrifuged and storedat )80 °C. Cells pellets were lysed in the presence of 0.4%N-lauroyl sarcosine [30] and ... addition of 0.1% (w ⁄ v) lysozyme and 0.5% (v ⁄ v) Triton X-100 during cell lysis.His6-ACA81)116was produced as described by Luoniet al. [31].Plant material and isolation of PM vesiclesCell ... in Arabidopsis and rice. Plant Physiol 132, 618–628.5 Aducci P, Camoni L, Marra M & Visconti S (2002) From cytosol to organelles: 14-3-3 proteins as multifunctionalregulators of plant cell....
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Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt
... homologues) in all kinds of organisms [1]. Extracellular peptidases hydrolyse largeproteins into smaller peptides for absorption by thecell, whereas intracellular peptidases play a major rolein ... FEBS Characterization of a recombinantly expressedproteinase K-like enzyme from a psychrotrophic Serratia sp.Atle Noralf Larsen1, Elin Moe1,2, Ronny Helland2, Dag Rune Gjellesvik3 and Nils Peder Willassen1,21 ... sources.Sequences from cold adapted as well as sequences of mesophilic and thermophilic origin are included.Figure 2A shows a multiple sequence alignment gener-ated by clustalx [27] of some of these...
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