... medium- vocabulary command and control task. 1 Introduction As the field of spoken language understanding be- comes more mature, a clearer picture begins to emerge of the tradeoffs between rule-based and data-driven ... with potential users and a short videotape of a session with a mock-up of the system; tight time con- strains and lack of access to users made it diffi- cul...
Ngày tải lên: 08/03/2014, 21:20
... Yuriy Fofanov 1,3 and Richard C. Willson 1,7 1 Department of Biology and Biochemistry, University of Houston, TX, USA 2 Department of Chemical and Biomolecular Engineering, University of Houston, ... screening for the presence of virus [14]. Nucleic acid-based assays offer rapid and specific detection and serotyping of dengue virus, and are gradually replacing serologic...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
... system to ascertain the conservation of targeting mechanisms and the nature of mitochondria-targeted proteins. Mitochondrial integrity and purity were established using electron microscopy, and ... NADPH, and 4 mm NDMA in a 1.0 mL final volume. The reaction mixture was incubated at 37 °C for 30 min, and the reaction was stopped by the addition of 0.1 mL of 25% zinc sulfat...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt
... differentiation, and so on. Isoforms of numerous proteins have been studied and compared in this man- ner, including alternative isoforms of members of the p53 ⁄ p63 ⁄ p73 [23] and the Bcl2 families ... versions of these two isoforms, as well as of the Mxi1-WR isoform that lacks the SID, and shown to give rise to proteins of the expected size expressed at similar levels (F...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
... suspen- ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed for 2 h. Products were extracted with 50 mL of hexane. The hexane layer was washed with 25 mL of saturated sodium ... range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity of skeletons in...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx
... of actin Rabbit actin was prepared by the method of Spudich and Watt [51]. Its molar concentration was determined by its absorbance at 290 nm using an E 1% of 6.3 cm )1 and a molecular mass of ... of Tm (a and b), each containing 284 residues. Smooth and skeletal muscles express differ- ent isoforms resulting from alternative splicing of the a and b genes. There are tw...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
... addition of 100 lLof2· Kirby buffer [25] and 80 lLofH 2 O, and rRNA was obtained by precipitation after two phenol– chloroform extractions. Four micrograms of rRNA were treatedwith20lL of acetic-aniline ... structure of such a hexanucleotide, where the conformation of the target adenine had been altered, using the known structures of complexes of RTA with FMP (formycin m...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... consumption and to examine the possible role played by cells and mole- cules of the immunological system in hepatic injury and repair. Understanding the interdependence between the metabolism of iron and ... Igs) and Super-Signal West Femto (Perbio). Densitometry quantification of the CD1d protein bands indicated was performed using a Kodak Digital Science DC40 camera and...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
... localization of cystatin F, its restricted and readily regulated expres- sion [18], selective and not too potent inhibition of cathepsins are all in favor of the active role of cystatin F in processes of ... lowered affinity of cyst- atin F for these enzymes. Proline in the S 2 site is a fea- ture of human stefin A and cystatins F, S and SN, all of which are significantly...
Ngày tải lên: 19/02/2014, 17:20
Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... Research Scholar of the Thailand Research Fund. We would like to thank Prof. Steve C. Fry, Institute of Cell and Molecular Biology, Univer- sity of Edinburgh, Edinburgh, UK, and Dr Albert Schulte, ... Center, Nakhon Ratchasima, Thailand 3 Department of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok, Thailand 4 Max...
Ngày tải lên: 20/02/2014, 01:20