... gelatine and unlessstated otherwise, all incubations were at 37 °C. PAI-1 wasincubated at a concentration of 20 lgÆmL)1 and at varioustime-points, aliquots were taken for preparation of a twofold ... half-life of PAI-1 was finally calculated from an exponential decay plot of the data obtained. Generally, only one preparation of each PAI-1 variant was investigated, but the following wereinvestigated ... preparations, givingindistinguishable results: wild-type, PAI-1(T9 6A) , PAI-1(F10 0A) , PAI-1(V12 6A) , PAI-1(F12 8A) , PAI-1(I13 7A) ,PAI-1(I13 8A) , PAI-1(N13 9A) , PAI-1(W14 1A) , PAI-1(T14 6A) and PAI-1(M149K).Structural...