Báo cáo khoa học: Identification of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx
Báo cáo khóa học: Identification of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen ppt
... osmoregulation. The discovery of the aquaporins marked a breakthrough in our understanding of water and solute transmembrane transport [1]. Aquaporins and aquaglyceroporins [the major intrinsic protein ... Sweden; 3 Swansea Clinical School, University of Wales Swansea, UK; 4 School of Life and Health Sciences, Aston University, Birmingham, UK Aquaporins and aquaglyceroporins media...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Identification of RNase HII from psychrotrophic bacterium, Shewanella sp. SIB1 as a high-activity type RNase H pot
... Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E, Nakamura H, Katayanagi K, Morikawa K & Ikehara M (1991) Stabilization of Escherichia coli ribonuclease H by introduction of an artificial ... 337–343. 47 Haruki M, Noguchi E, Akasako A, Oobatake M, Itaya M & Kanaya S (1994) A novel strategy for stabilization of Escherichia coli ribonuclease HI involving a screen for...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Identification of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx
... G GAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC 3+ wt GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC 3+ YIRN GGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG 3– GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGG Ó FEBS 2003 A novel ... GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG 2a+ GACTGCAaCCCCAAAtCGGACAG 2b+ CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTG...
Ngày tải lên: 17/03/2014, 03:20
Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
... cerevisiae strain GIL77 Two oligo DNAs (5¢-CTTCGTCGACAAGATGTGGAG GTTGAAGATA-3¢ and 5¢-GTCCGCTAGCTCAAGGCA AAGGAACTCTTCT-3¢), corresponding to the N- and C-terminal sequences of b-amyrin synthase from ... Cabello-Hurtado F, Zimmerlin A, Rahier A, Taton M, DeRose R, Nedelkina S, Batard Y, Durst F, Pallett KE & Werck-Reichhart D (1997) Cloning and functional expression in yeast of...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
... phosphorylation was a result of other protein kinases masking the PKA-catalysed part. Indeed, protein kinases other than PKA, including tyrosine kinases, have been demonstrated to directly phosphorylate ... phosphorylation One microgram of the appropriate protein was incubated in 300 lL of PhB containing 18.5 kBq [ 32 P]ATP[cP] and 0.4 lL of PKA-cs for 30 min at room temperature...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx
... effect of citrate, key intermedi- ates (pyruvate, acetyl-CoA, acetyl phosphate and CoA) and major end products (acetate and lactate) of citrate and malate metabolism. These assays indicated that ... the absence of malic activity for CitM may be a result of an improper ori- entation of the cofactor that impairs catalysis or malate binding, as was described for site- directed...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: Identification of the heparin-binding domains of the interferon-induced protein kinase, PKR pptx
... to the instructions provided with the kit: K29 9A: 5¢-GTTATTAAACGTGTTGCATATAATAAC GAG-3¢; DM: 5¢-ACTTACGTTATTGCACGTGTTGCAT ATAATAACGAG-3¢; hep2: 5¢-CTGAAAAATGATGGAG AGCTCACAGAGAGTAAGGGAACTTTG-3¢. The ... amino- terminal heparin -binding domain (ATD) of PKR. Thus, in contrast to the dsRNA -binding domain of PKR, its heparin -binding domains are located in the carboxy-terminal half...
Ngày tải lên: 23/03/2014, 13:20
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... C), for pGEX–EWS; EAD forward d(CGG AAT TCA TGG CGT CCA CGG ATT ACA G) and EAD reverse d(CGC TCG AGT CAT CCG GAA AAT CCT CCA GAC T), for pGEX–EAD; RGG1 forward d(CGG AAT TCC CAG GAG AGA ACC GGA ... protein as a G-quadruplex DNA- and RNA -binding protein Kentaro Takahama 1, *, Katsuhito Kino 2, *, Shigeki Arai 3 , Riki Kurokawa 3 and Takanori Oyoshi 1 1 Department of Chemistry, Facult...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
... Catlin BW (1990) Branhamella catarrhalis: an organism gaining respect as a pathogen. Clin Microbiol Rev 3, 293–320. 2 Karalus R & Campagnari A (2000) Moraxella catarrh- alis: a review of an ... AAG CCG ATG ACA CCA ATT (asd sense) This study asd2 GCA GGT TCA TAG TGC ATG (asd antisense) This study Kan RP GGT GCG ACA ATC TAT CGA (kanamycin sense) [19] Kan FP CTC ATC GAG CAT CAA ATG (...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... immunoscreening of a cDNA library was per- formed using this antiserum. Approximately 200 000 clones contained in a kZAP II (Stratagene, La Jolla, CA, USA) inner ear cDNA library, constructed according ... 126, 511–520. 27 Tohse H, Murayama E, Ohira T, Takagi Y & Nagasa- wa H (2006) Localization and diurnal variations of car- bonic anhydrase mRNA expression in the inner ear o...
Ngày tải lên: 18/02/2014, 17:20