Báo cáo khoa học: Interaction of the P-type cardiotoxin with phospholipid membranes pptx
Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... differently with HSP25 so we analyzed the effect of HSP25 on the polymerization of intact actin andaggregation of heated actin. Interaction of HSP25 with intact actinIn the first series of experiments ... appearance of an additional peak at 7.65 mLon the elution profile. Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and decrease of the ... electrophoresis. The quantity of actin in the pellet was plotted against the totalquantity of actin in the sample.ResultsHeat denaturation of actinBefore starting the investigation of the HSP25–actin...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP.On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... either a stable soluble complex with GlnK in the absence of AmtB, or constitutively binds to GS in the absence of GlnK. In vitro, the TnrA C-terminus is responsible for interactions with either ... with ATP and Mg2+or Mn2+. To resolve the inhibitoryeffect of ATP on the GlnK–TnrA interaction in the absence of divalent cations more clearly, ATP wastitrated to the binding assays in the...
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Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt
... associ-ation with one PSBD close to the twofold axis of E3preventing the association of a second PSBD [28,29]. The interaction occurs at the C2-axis of symmetry in the E3 dimer, chiefly with the interface ... the basis of the interaction of E2p with E3 in the assem-bly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearo-thermophilus. Thirteen of the 55 amino acids in the PSBD show ... in either or both of the 15N and1H amide chemical shifts when the PSBD forms a 1 : 1 complex with E3int. All of the 13 amino acidsreside near the N-terminus of helix I of PSBD or in the loop...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
... (CLR*).Incubation of T4 DNA with CLR* in the presence of fucoidan (CLR/fucoidan molar ratio of 1 : 20 and 1 : 40)leads to an inhibition of the binding of CLR* to DNA from the molar ratio 1 : 20. Altogether these ... with some DNA strand (Fig. 3). This lower inhibition may bedue to the presence of the catalytic tetramer C1r2–C1s2in the C1 complex that hinders the interaction of the C1qsubunit with the ... overlap with the binding site of the tetramer in the hinge region. It is likely that DNAbinds to the stalk domain of CLR, consistent with previousfindings showing that a synthetic peptide of the...
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Báo cáo khoa học: Interaction of the P-type cardiotoxin with phospholipid membranes pptx
... extinguishes the temperature dependence of the deformation of Pam2Gro-PGro MLV observed in the absence of the toxin. These datasupport the above conclusion on hampering of the defor-mation of Pam2Gro-PGro ... membrane the hydrophobic partitioning of CTII is favoured. In the case of the P-type CTs the insertion is accomplished via the tips of three hydrophobic loops (fingers) of the molecule[14–16,43]. The ... from the stiffening of lipidmolecules in the polar but not in the acyl region of the membrane. (In this respect the influence of CTs on the membrane stiffness is opposite to the action of the cholesterol...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... of the first 88amino acids of PPI1 may participate in the interaction with the H+-ATPase and makes PPI1588His6a suitabletool to study the mechanism of action of PPI1. The analysis of the ... 14-3-3s.Results The C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts with the first 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPasebinding site of PPI1 is ... interact with A. thaliana H+-ATPase in the PM and stimulate itsactivity [22], suggested that the site of interaction with the PM H+-ATPase was localized in the N-terminus of PPI1. To further...
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Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc
... in the absence of Ca2+, inde-pendent of the addition of T800. This result, together with the fact that T800 increased the average andmaximum velocity v alues of moving filaments, both in the ... remained in the pellet independently of the other components and the ionic strength of the mixture.In the presence o f A TP, the amount of bou nd H MM wasvery similar (average value of 52.5 ± ... solution. On the other hand, the acceleration of the sliding velocity (and the lower number of stops) of thin filaments could be related toa diminution o f the load in the actin–myosin interface.The...
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Báo cáo khóa học: Disruption of the interaction between the Rieske iron–sulfur protein and cytochrome b in the yeast bc1 complex owing to a human disease-associated mutation within cytochrome b potx
... replacement of this residue with glutamate could interfere with the movement of theISP, which would, in turn, alter the catalyticactivity of the complex. The mutation could also affect the binding of the ... below.Instability of the mutant enzymeFurther analysis of the kinetic parameters of the G167Emutant was hindered by rapid inhibition of the activity of the bc1complex during the assay, which ... into the mitochondria. Studies of the mechanism of assembly of the bc1complex suggest that the ISP is one of the lastsubunits to be integrated within the membrane-boundsubcomplex [2]. The...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... we measured the interaction of stefin B with various combinations of phospholipid monolayersand bilayers. Interaction of stefin B in the prefibrillaraggregated state with model lipid membranes wasprobed ... mol ⁄mol). The results are mean ± SD, n ¼ 1–4. The degree of permea-bilization is expressed as the percentage of the maximal valueobtained at the end of the assay by the addition of 2 mM TritonX-100. ... monolayers The ability of stefins and their aggregates to insert at the air–water interface, i.e. in the absence of lipids,was determined first, as this may give an indicationabout the amphipathicity of...
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Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx
... systemically within the hostplant [11]. The present study comprised biochemicalcharacterization of the MP encoded by ORF1 of SeMV, a member of the genus Sobemovirus. Analysis of the deduced ... degree of interaction between the pro-teins remains. It is possible that another site of interaction beyond the N-terminal 49 residues maycontribute to these weak interactions.In conclusion, the ... mutants of MP were performed to determine the domain responsible for the interaction of MP with CPusing ELISA and yeast two-hybrid (Y2H) assays.Deletion of the N-terminal 49 amino acids of the SeMV...
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