Báo cáo khoa học: Interaction of the P-type cardiotoxin with phospholipid membranes pptx

... differently with HSP25 so we analyzed the effect of HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the first series of experiments ... appearance of an additional peak at 7.65 mL on the elution profile. Increase of the temperature of incubation was accompanied by the simultaneous increase...

Ngày tải lên: 20/02/2014, 23:20

... more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP. On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... either a stable soluble complex with GlnK in the absence of AmtB, or constitutively binds to GS in the absence of GlnK. In vitro, the TnrA C-terminus is responsi...

Ngày tải lên: 06/03/2014, 00:21

... associ- ation with one PSBD close to the twofold axis of E3 preventing the association of a second PSBD [28,29]. The interaction occurs at the C 2 -axis of symmetry in the E3 dimer, chiefly with the interface ... the basis of the interaction of E2p with E3 in the assem- bly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearo- thermop...

Ngày tải lên: 16/03/2014, 18:20

... (CLR*). Incubation of T4 DNA with CLR* in the presence of fucoidan (CLR/fucoidan molar ratio of 1 : 20 and 1 : 40) leads to an inhibition of the binding of CLR* to DNA from the molar ratio 1 : 20. Altogether these ... with some DNA strand (Fig. 3). This lower inhibition may be due to the presence of the catalytic tetramer C1r 2 –C1s 2 in the C1 complex that hinders...

Ngày tải lên: 16/03/2014, 23:20

... extinguishes the temperature dependence of the deformation of Pam 2 Gro- PGro MLV observed in the absence of the toxin. These data support the above conclusion on hampering of the defor- mation of Pam 2 Gro-PGro ... membrane the hydrophobic partitioning of CTII is favoured. In the case of the P-type CTs the insertion is accomplished via the tips of thre...

Ngày tải lên: 17/03/2014, 03:20

... of the first 88 amino acids of PPI1 may participate in the interaction with the H + -ATPase and makes PPI1 588 His 6 a suitable tool to study the mechanism of action of PPI1. The analysis of the ... 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating th...

Ngày tải lên: 19/02/2014, 07:20

... in the absence of Ca 2+ , inde- pendent of the addition of T800. This result, together with the fact that T800 increased the average and maximum velocity v alues of moving filaments, both in the ... remained in the pellet independently of the other components and the ionic strength of the mixture. In the presence o f A TP, the amount of bou nd H MM was ver...

Ngày tải lên: 19/02/2014, 16:20

... replacement of this residue with glutamate could interfere with the movement of theISP, which would, in turn, alter the catalytic activity of the complex. The mutation could also affect the binding of the ... below. Instability of the mutant enzyme Further analysis of the kinetic parameters of the G167E mutant was hindered by rapid inhibition of the act...

Ngày tải lên: 07/03/2014, 15:20

... we measured the interaction of stefin B with various combinations of phospholipid monolayers and bilayers. Interaction of stefin B in the prefibrillar aggregated state with model lipid membranes was probed ... mol ⁄ mol). The results are mean ± SD, n ¼ 1–4. The degree of permea- bilization is expressed as the percentage of the maximal value obtained at the end...

Ngày tải lên: 07/03/2014, 17:20

... systemically within the host plant [11]. The present study comprised biochemical characterization of the MP encoded by ORF1 of SeMV, a member of the genus Sobemovirus. Analysis of the deduced ... degree of interaction between the pro- teins remains. It is possible that another site of interaction beyond the N-terminal 49 residues may contribute to these weak inte...

Ngày tải lên: 15/03/2014, 00:20